FastQCFastQC Report
Thu 9 Feb 2017
SRR2727556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751905
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12950.021.57142837
GGTATCA10800.020.5555551
TACTTAC7150.018.11188931
TAGACTA650.001580381217.0769235
CGTTACG6000.016.95833224
GTACTTA7900.016.62658130
TCTTATA22950.016.12200537
TCGTTAC7000.016.12142823
CTCGTTA7350.015.85714322
GCTTATA3850.015.8571421
AGTGTAC9650.015.52849727
ACTTACA6050.015.28925632
ATCTCGT8750.015.01142920
GTGTACT9850.014.837563528
ATTATAC1651.6465492E-814.5757573
GTAGTAA908.277066E-414.3888891
CTTATAG4250.014.3647062
TACGGAT11700.014.23076827
GATCTCG9250.014.219
TGTACCG1854.9021764E-914.05
Produced by FastQC (version 0.11.5)