##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727556.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1751905
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65253252887571	34.0	31.0	34.0	31.0	34.0
2	32.82611385891358	34.0	31.0	34.0	31.0	34.0
3	32.91841224267298	34.0	31.0	34.0	31.0	34.0
4	36.35637605920412	37.0	37.0	37.0	35.0	37.0
5	36.22437803419706	37.0	35.0	37.0	35.0	37.0
6	36.24164552301637	37.0	37.0	37.0	35.0	37.0
7	36.222567433736415	37.0	36.0	37.0	35.0	37.0
8	36.21137904167178	37.0	36.0	37.0	35.0	37.0
9	37.93368761433982	39.0	38.0	39.0	35.0	39.0
10	37.90794877576124	39.0	38.0	39.0	35.0	39.0
11	37.98493354377092	39.0	38.0	39.0	35.0	39.0
12	37.96509571009844	39.0	38.0	39.0	35.0	39.0
13	37.99375651076971	39.0	38.0	39.0	35.0	39.0
14	39.36656725107811	40.0	39.0	41.0	37.0	41.0
15	39.39233291759542	40.0	39.0	41.0	37.0	41.0
16	39.37295458372457	40.0	39.0	41.0	37.0	41.0
17	39.391940202236995	40.0	39.0	41.0	37.0	41.0
18	39.3655243863109	40.0	39.0	41.0	36.0	41.0
19	39.40670470145356	40.0	39.0	41.0	37.0	41.0
20	39.380538328276934	40.0	39.0	41.0	37.0	41.0
21	39.365894840188254	40.0	39.0	41.0	37.0	41.0
22	39.33856744515256	40.0	39.0	41.0	36.0	41.0
23	39.29999286490991	40.0	39.0	41.0	36.0	41.0
24	39.26641570176465	40.0	39.0	41.0	36.0	41.0
25	39.20608023836909	40.0	39.0	41.0	36.0	41.0
26	39.11854923640266	40.0	39.0	41.0	36.0	41.0
27	39.080814313561525	40.0	39.0	41.0	36.0	41.0
28	39.01543805172084	40.0	39.0	41.0	36.0	41.0
29	38.94742979784863	40.0	38.0	41.0	35.0	41.0
30	38.899829614048706	40.0	38.0	41.0	35.0	41.0
31	38.833979582226206	40.0	38.0	41.0	35.0	41.0
32	38.75536401802609	40.0	38.0	41.0	35.0	41.0
33	38.713929122869104	40.0	38.0	41.0	35.0	41.0
34	38.68038963299951	40.0	38.0	41.0	35.0	41.0
35	38.677624072081535	40.0	38.0	41.0	35.0	41.0
36	38.61930869539159	40.0	38.0	41.0	35.0	41.0
37	38.61030820735142	40.0	38.0	41.0	35.0	41.0
38	38.5778412642238	40.0	38.0	41.0	35.0	41.0
39	38.53466026982057	40.0	38.0	41.0	35.0	41.0
40	38.48911442115868	40.0	38.0	41.0	35.0	41.0
41	38.445532149288915	40.0	38.0	41.0	34.0	41.0
42	38.418161943712704	40.0	38.0	41.0	34.0	41.0
43	37.36656097219883	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	2.0
17	7.0
18	12.0
19	27.0
20	47.0
21	123.0
22	274.0
23	494.0
24	883.0
25	1459.0
26	2296.0
27	3573.0
28	5352.0
29	7868.0
30	11167.0
31	15858.0
32	22127.0
33	30800.0
34	44960.0
35	66958.0
36	104024.0
37	181590.0
38	444060.0
39	807942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.908491042607906	16.82596944469021	12.291933637954113	27.973605874747776
2	18.128836894694633	19.360924251029594	36.23113125426322	26.27910760001256
3	19.72230229378876	21.19806724679706	29.751955728193025	29.327674731221155
4	15.312188731694926	14.586464448700129	33.405635579554826	36.695711240050116
5	15.895154132216074	35.362362685191265	33.45586661377187	15.286616568820799
6	36.78926654127935	33.013947674103335	14.978437757755128	15.218348026862188
7	30.54286619422857	30.20894397812667	20.199725441733428	19.048464385911338
8	27.6749595440392	35.24146571874616	19.010163222320845	18.073411514893785
9	29.015100704661496	14.105159811747784	17.475776369152438	39.403963114438284
10	16.774425553897043	26.497213033811768	32.42270556908052	24.305655843210676
11	37.851652914969705	20.990921311372475	20.16553409003342	20.9918916836244
12	22.332147005688093	23.187330363233166	29.57877282158565	24.901749809493097
13	31.7637086485854	19.076890584820525	23.08292972507071	26.07647104152337
14	23.112782941997427	19.002856890071094	24.57673218582058	33.3076279821109
15	26.672679169247193	26.279050519291857	21.24156275597136	25.806707555489595
16	25.326658694392673	25.4585722399331	23.603391736424065	25.611377329250157
17	25.249085994959774	24.749972173148656	24.057811353926155	25.943130477965415
18	25.3763760021234	25.26660977621503	24.58900454077133	24.76800968089023
19	26.859789771705657	24.52279090475796	24.359825447156098	24.257593876380284
20	25.6278165768121	23.940282150002425	24.503155136836757	25.928746136348717
21	25.26381282090068	24.87263864193549	25.29691963890736	24.56662889825647
22	26.295375605412396	23.625082410290513	23.840562130937464	26.238979853359627
23	25.397552949503538	24.276715917815178	24.865046906082235	25.460684226599046
24	24.957917238663054	23.667493385771486	25.06323116835673	26.311358207208723
25	25.650363461489068	23.531812512664786	24.414851261912034	26.40297276393412
26	25.269178408646585	24.02858602492715	24.951124632899617	25.751110933526643
27	25.942160105713498	24.481921108735918	24.229224758191798	25.34669402735879
28	25.28156492503874	25.411708968237434	23.29898025292467	26.007745853799154
29	25.31392969367631	25.105356740234203	24.647341037327937	24.93337252876155
30	26.85859107657093	24.096626244002955	23.517827736092997	25.526954943333113
31	25.566112317734124	24.271578652952073	24.78387812124516	25.378430908068644
32	25.16472068976343	24.57593305573076	24.44282081505561	25.8165254394502
33	24.991594863876752	23.751915771688534	24.668517984708075	26.58797137972664
34	25.57090709827302	24.035207388528487	25.55749312890824	24.836392384290242
35	25.484886452176347	23.63986631695212	24.844212443026308	26.03103478784523
36	25.17094248831986	23.428838892519856	25.682671149405934	25.717547469754354
37	24.326090741221698	23.335797317776937	26.21717501805178	26.12093692294959
38	25.37283699743993	22.56183982578964	25.78079290829126	26.284530268479166
39	23.953924442250006	23.208735633496108	27.135033006926747	25.70230691732714
40	25.404802201032588	22.11078797080892	26.941072717984138	25.543337110174352
41	24.078132090495775	22.968597041506246	27.087427685861957	25.865843182136018
42	23.620401791193014	22.93880090530023	27.53157277363784	25.90922452986891
43	23.07436761696553	22.683992568090165	27.01476392840936	27.226875886534945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	73.5
2	103.0
3	183.0
4	263.0
5	263.0
6	327.5
7	392.0
8	380.0
9	368.0
10	512.0
11	656.0
12	656.0
13	1131.5
14	1607.0
15	2244.0
16	2881.0
17	2792.5
18	2704.0
19	2704.0
20	3323.0
21	3942.0
22	4316.0
23	4690.0
24	6222.0
25	7754.0
26	7754.0
27	10004.5
28	12255.0
29	14901.5
30	17548.0
31	22541.0
32	27534.0
33	27534.0
34	33940.5
35	40347.0
36	46583.5
37	52820.0
38	60449.0
39	68078.0
40	68078.0
41	77229.5
42	86381.0
43	97693.0
44	109005.0
45	122940.0
46	136875.0
47	136875.0
48	150438.5
49	164002.0
50	173355.5
51	182709.0
52	180691.5
53	178674.0
54	178674.0
55	168765.0
56	158856.0
57	147449.5
58	136043.0
59	125698.5
60	115354.0
61	115354.0
62	100444.0
63	85534.0
64	71969.5
65	58405.0
66	48181.5
67	37958.0
68	37958.0
69	30956.0
70	23954.0
71	19247.0
72	14540.0
73	11700.0
74	8860.0
75	8860.0
76	7041.5
77	5223.0
78	4091.5
79	2960.0
80	2230.0
81	1500.0
82	1500.0
83	1073.0
84	646.0
85	465.0
86	284.0
87	194.0
88	104.0
89	104.0
90	72.5
91	41.0
92	25.0
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1751905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.12483290417819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.11704374241125	57.30459549345214
2	8.994373408233464	12.254803710273766
3	2.815955099000008	5.755094117551323
4	1.3257909849416094	3.6127715726005185
5	0.7525939528739706	2.56351686421171
6	0.4560900703026686	1.8642635897174507
7	0.31647827381355065	1.5092020664945622
8	0.2273714666843747	1.2391714540040755
9	0.17304925878394983	1.0610056654963622
>10	0.7752977143641682	8.664600209242888
>50	0.028576424080627064	1.3325652044416354
>100	0.016036514441260877	2.1116316968540145
>500	0.0010073175518345993	0.4668674557875438
>1k	3.357725172781996E-4	0.2599108998719238
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.708072070117957E-5	0.0
2	0.0	0.0	0.0	1.1416144140235914E-4	0.0
3	0.0	0.0	0.0	1.1416144140235914E-4	0.0
4	0.0	0.0	0.0	1.1416144140235914E-4	1.1416144140235914E-4
5	0.0	0.0	0.0	1.712421621035387E-4	1.1416144140235914E-4
6	0.0	0.0	0.0	1.712421621035387E-4	2.2832288280471828E-4
7	0.0	0.0	0.0	1.712421621035387E-4	2.2832288280471828E-4
8	0.0	0.0	0.0	2.2832288280471828E-4	2.2832288280471828E-4
9	0.0	0.0	0.0	2.2832288280471828E-4	2.8540360350589786E-4
10	0.0	0.0	0.0	2.8540360350589786E-4	2.8540360350589786E-4
11	0.0	0.0	0.0	3.424843242070774E-4	2.8540360350589786E-4
12	0.0	0.0	0.0	3.424843242070774E-4	2.8540360350589786E-4
13	0.0	0.0	0.0	3.424843242070774E-4	2.8540360350589786E-4
14	0.0	0.0	0.0	3.424843242070774E-4	3.424843242070774E-4
15	0.0	0.0	0.0	3.424843242070774E-4	5.137264863106162E-4
16	0.0	0.0	0.0	4.5664576560943657E-4	5.137264863106162E-4
17	0.0	0.0	0.0	6.849686484141548E-4	5.137264863106162E-4
18	0.0	0.0	0.0	6.849686484141548E-4	5.137264863106162E-4
19	0.0	0.0	0.0	7.420493691153345E-4	5.137264863106162E-4
20	0.0	0.0	0.0	0.0011416144140235914	5.137264863106162E-4
21	0.0	0.0	0.0	0.001484098738230669	5.137264863106162E-4
22	0.0	0.0	0.0	0.0019407445038401054	5.137264863106162E-4
23	0.0	0.0	0.0	0.0022261481073460035	5.708072070117957E-4
24	0.0	0.0	0.0	0.002511551710851901	5.708072070117957E-4
25	0.0	0.0	0.0	0.0030252781971625174	5.708072070117957E-4
26	0.0	0.0	0.0	0.004851861259600264	5.708072070117957E-4
27	0.0	0.0	0.0	0.015411794589318484	6.278879277129753E-4
28	0.0	0.0	0.0	0.054397926828224134	6.278879277129753E-4
29	0.0	0.0	0.0	0.11490349077147448	6.278879277129753E-4
30	0.0	0.0	0.0	0.1933894817355964	6.278879277129753E-4
31	0.0	0.0	0.0	0.6063684960086306	6.278879277129753E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1295	0.0	21.571428	37
GGTATCA	1080	0.0	20.555555	1
TACTTAC	715	0.0	18.111889	31
TAGACTA	65	0.0015803812	17.076923	5
CGTTACG	600	0.0	16.958332	24
GTACTTA	790	0.0	16.626581	30
TCTTATA	2295	0.0	16.122005	37
TCGTTAC	700	0.0	16.121428	23
CTCGTTA	735	0.0	15.857143	22
GCTTATA	385	0.0	15.857142	1
AGTGTAC	965	0.0	15.528497	27
ACTTACA	605	0.0	15.289256	32
ATCTCGT	875	0.0	15.011429	20
GTGTACT	985	0.0	14.8375635	28
ATTATAC	165	1.6465492E-8	14.575757	3
GTAGTAA	90	8.277066E-4	14.388889	1
CTTATAG	425	0.0	14.364706	2
TACGGAT	1170	0.0	14.230768	27
GATCTCG	925	0.0	14.2	19
TGTACCG	185	4.9021764E-9	14.0	5
>>END_MODULE