##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502070
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.651467641321645	34.0	31.0	34.0	31.0	34.0
2	32.8204544395401	34.0	31.0	34.0	31.0	34.0
3	32.908204011797054	34.0	31.0	34.0	31.0	34.0
4	36.349513671133835	37.0	37.0	37.0	35.0	37.0
5	36.215781554787725	37.0	35.0	37.0	35.0	37.0
6	36.23414621156138	37.0	36.0	37.0	35.0	37.0
7	36.20995759185657	37.0	36.0	37.0	35.0	37.0
8	36.20491987723608	37.0	36.0	37.0	35.0	37.0
9	37.944444000612485	39.0	38.0	39.0	35.0	39.0
10	37.90217233551033	39.0	38.0	39.0	35.0	39.0
11	37.98735744672352	39.0	38.0	39.0	35.0	39.0
12	37.96008241959429	39.0	38.0	39.0	35.0	39.0
13	37.992308614112524	39.0	38.0	39.0	35.0	39.0
14	39.35260207580206	40.0	39.0	41.0	37.0	41.0
15	39.378411791727416	40.0	39.0	41.0	37.0	41.0
16	39.34978662778699	40.0	39.0	41.0	36.0	41.0
17	39.37831059804137	40.0	39.0	41.0	37.0	41.0
18	39.33395713914798	40.0	39.0	41.0	36.0	41.0
19	39.39159160358705	40.0	39.0	41.0	37.0	41.0
20	39.353074091087635	40.0	39.0	41.0	37.0	41.0
21	39.34125773099789	40.0	39.0	41.0	36.0	41.0
22	39.32040317694914	40.0	39.0	41.0	36.0	41.0
23	39.281952904991115	40.0	39.0	41.0	36.0	41.0
24	39.2520222093511	40.0	39.0	41.0	36.0	41.0
25	39.18130779524257	40.0	39.0	41.0	36.0	41.0
26	39.092885151823815	40.0	39.0	41.0	36.0	41.0
27	39.05777826599293	40.0	39.0	41.0	36.0	41.0
28	38.95846132337375	40.0	38.0	41.0	35.0	41.0
29	38.892610197926864	40.0	38.0	41.0	35.0	41.0
30	38.85694075509131	40.0	38.0	41.0	35.0	41.0
31	38.78859573788172	40.0	38.0	41.0	35.0	41.0
32	38.70680594113457	40.0	38.0	41.0	35.0	41.0
33	38.66773718934537	40.0	38.0	41.0	35.0	41.0
34	38.62789284121246	40.0	38.0	41.0	35.0	41.0
35	38.63327541326303	40.0	38.0	41.0	35.0	41.0
36	38.54262584300332	40.0	38.0	41.0	35.0	41.0
37	38.55820301317515	40.0	38.0	41.0	35.0	41.0
38	38.53126418875286	40.0	38.0	41.0	35.0	41.0
39	38.46462082326389	40.0	38.0	41.0	34.0	41.0
40	38.44076840626602	40.0	38.0	41.0	34.0	41.0
41	38.37786388117731	40.0	38.0	41.0	34.0	41.0
42	38.36255767041483	40.0	38.0	41.0	34.0	41.0
43	37.27322361807372	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	0.0
14	0.0
15	1.0
16	2.0
17	4.0
18	12.0
19	20.0
20	57.0
21	132.0
22	239.0
23	401.0
24	762.0
25	1280.0
26	1885.0
27	2984.0
28	4444.0
29	6704.0
30	9511.0
31	13620.0
32	18933.0
33	26541.0
34	39941.0
35	59951.0
36	92628.0
37	159700.0
38	390581.0
39	671731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.819129601150415	15.780622740617947	11.806973043866131	28.59327461436551
2	19.072679702011225	19.020751363118897	35.19156896815728	26.714999966712604
3	20.190403909271872	20.782719846611673	29.68623299846212	29.34064324565433
4	16.223544841452128	13.9729839488173	32.170071967351724	37.63339924237885
5	16.600158448008415	35.374849374529816	32.382245834082305	15.642746343379468
6	38.274447928525305	31.42157156457422	14.658038573435325	15.645941933465151
7	31.06779311217187	30.415226986758277	19.56227073305505	18.954709168014805
8	28.692071607847836	34.78499670454772	18.751988921954368	17.77094276565007
9	30.626069357619816	13.37740584659836	16.401432689555083	39.595092106226744
10	17.062187514563238	25.95618047095009	32.02547151597462	24.95616049851205
11	38.90837311177242	20.07849168147956	19.613932772773573	21.399202433974448
12	22.75899259022549	22.36946347373957	28.593074889985154	26.278469046049786
13	32.517259515202355	18.40919531047155	22.525181915623108	26.548363258702988
14	23.875185577236746	18.666240587988575	23.441717097072708	34.01685673770197
15	28.188566444972608	25.656327601243618	20.455438162003105	25.699667791780676
16	25.665182048772696	24.908958969954796	23.36602155691812	26.05983742435439
17	26.228604525754456	23.380934310651302	22.785089909258556	27.60537125433568
18	25.630363431797456	25.413263030351445	23.68757780928985	25.26879572856125
19	28.31778811906236	23.64317242205756	23.29924703908606	24.739792419794018
20	26.046921914424757	22.93248650196063	23.53605357939377	27.484538004220845
21	25.791873880711286	24.230761549062294	25.263070296324408	24.714294273902016
22	27.247332015152427	22.34556312289041	22.68276445172329	27.72434041023388
23	25.770902820774	23.728055283708482	24.609239249835227	25.891802645682294
24	24.934390541053347	22.744146411285758	24.7077033693503	27.613759678310597
25	26.497699840886245	22.230521879805867	23.632720179485645	27.639058099822243
26	25.75585691745391	23.059444633073024	24.575885278315923	26.608813171157138
27	27.541459452622046	23.76234130233611	23.070762347959818	25.625436897082025
28	25.529835493685383	25.416658344817485	21.712902860718874	27.34060330077826
29	26.0798098623899	24.821879140119968	23.856211761103012	25.242099236387116
30	28.74413309632707	22.84041356261692	22.29469998069331	26.1207533603627
31	26.660342061288755	23.51375102358745	24.23688643005985	25.589020485063944
32	26.209763859207623	23.689441903506495	23.49084929464006	26.609944942645814
33	25.73927979388444	22.583368285099894	23.86087199664463	27.816479924371034
34	26.66713269022083	23.394781867689254	25.052960248190832	24.885125193899086
35	26.729446696891625	22.757661094356454	23.731317448587617	26.781574760164307
36	25.805055689814722	22.58869426857603	25.005958444013928	26.600291597595316
37	24.925269794350463	22.96610677265374	25.951786534582276	26.15683689841352
38	26.890158248284035	21.532684894845115	24.495795801793523	27.081361055077323
39	24.22350489657606	23.235668111339685	26.63630856085269	25.904518431231566
40	27.148468446876645	21.05334638199285	25.783951480290533	26.014233690839973
41	24.96568069397565	22.768313061308728	25.90265433701492	26.363351907700704
42	24.7938511520768	22.588095095434966	26.500895430971926	26.11715832151631
43	23.897421558249615	21.995046835367194	25.507732662259414	28.599798944123776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	30.5
2	43.0
3	77.5
4	112.0
5	112.0
6	134.5
7	157.0
8	140.0
9	123.0
10	178.0
11	233.0
12	233.0
13	358.0
14	483.0
15	629.0
16	775.0
17	814.0
18	853.0
19	853.0
20	1124.0
21	1395.0
22	1680.0
23	1965.0
24	2745.0
25	3525.0
26	3525.0
27	4636.0
28	5747.0
29	7203.5
30	8660.0
31	11285.0
32	13910.0
33	13910.0
34	17451.0
35	20992.0
36	25824.5
37	30657.0
38	37030.5
39	43404.0
40	43404.0
41	51134.5
42	58865.0
43	69200.5
44	79536.0
45	96093.0
46	112650.0
47	112650.0
48	130651.5
49	148653.0
50	163080.0
51	177507.0
52	177609.5
53	177712.0
54	177712.0
55	164078.0
56	150444.0
57	139364.0
58	128284.0
59	119714.0
60	111144.0
61	111144.0
62	96629.0
63	82114.0
64	68271.0
65	54428.0
66	44763.5
67	35099.0
68	35099.0
69	28534.5
70	21970.0
71	17525.0
72	13080.0
73	10534.0
74	7988.0
75	7988.0
76	6312.0
77	4636.0
78	3605.0
79	2574.0
80	1973.0
81	1372.0
82	1372.0
83	966.0
84	560.0
85	399.0
86	238.0
87	176.0
88	114.0
89	114.0
90	73.5
91	33.0
92	21.5
93	10.0
94	7.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1502070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.61947952473723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29850478854513	49.888925259457
2	9.301825873623276	11.277436861775541
3	3.1121155130455183	5.659644678650396
4	1.532157261167334	3.7151430288804272
5	0.9293876720081253	2.8169498476919874
6	0.611266836708283	2.223280649519319
7	0.4084552735612963	1.733224226469396
8	0.3116099910941594	1.5111708379868753
9	0.23242196896598977	1.2680368909949633
>10	1.1744430595767388	12.081675178202737
>50	0.05047908008312963	2.06664924035189
>100	0.03403872017354679	4.056271995961882
>500	0.0025253704430872877	1.034532901974102
>1k	7.685910044178702E-4	0.6670584020835394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTA	1975	0.13148521706711405	No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC	1896	0.12622580838442948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.657479345170331E-5	0.0
5	0.0	0.0	0.0	6.657479345170331E-5	0.0
6	0.0	0.0	0.0	6.657479345170331E-5	6.657479345170331E-5
7	0.0	0.0	0.0	1.9972438035510996E-4	6.657479345170331E-5
8	0.0	0.0	0.0	1.9972438035510996E-4	6.657479345170331E-5
9	0.0	0.0	0.0	2.6629917380681324E-4	6.657479345170331E-5
10	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	6.657479345170331E-5
11	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	1.3314958690340662E-4
12	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	1.9972438035510996E-4
13	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	1.9972438035510996E-4
14	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	2.6629917380681324E-4
15	0.0	6.657479345170331E-5	0.0	2.6629917380681324E-4	3.328739672585166E-4
16	0.0	6.657479345170331E-5	0.0	3.328739672585166E-4	3.994487607102199E-4
17	0.0	6.657479345170331E-5	0.0	4.660235541619232E-4	3.994487607102199E-4
18	0.0	6.657479345170331E-5	0.0	5.991731410653298E-4	3.994487607102199E-4
19	0.0	6.657479345170331E-5	0.0	5.991731410653298E-4	4.660235541619232E-4
20	0.0	6.657479345170331E-5	0.0	9.986219017755497E-4	4.660235541619232E-4
21	0.0	6.657479345170331E-5	0.0	0.0015312202493891762	4.660235541619232E-4
22	0.0	6.657479345170331E-5	0.0	0.0023966925642613193	4.660235541619232E-4
23	0.0	6.657479345170331E-5	0.0	0.002796141324971539	5.991731410653298E-4
24	0.0	6.657479345170331E-5	0.0	0.0038613380201987925	5.991731410653298E-4
25	0.0	6.657479345170331E-5	0.0	0.004527085954715826	6.657479345170332E-4
26	0.0	6.657479345170331E-5	0.0	0.005925156617201595	7.323227279687365E-4
27	0.0	6.657479345170331E-5	0.0	0.012715785549275334	7.323227279687365E-4
28	0.0	6.657479345170331E-5	0.0	0.04393936367812419	7.323227279687365E-4
29	0.0	6.657479345170331E-5	0.0	0.09247238810441591	7.988975214204398E-4
30	0.0	6.657479345170331E-5	0.0	0.15918033114302263	7.988975214204398E-4
31	0.0	6.657479345170331E-5	0.0	0.49325264468366986	7.988975214204398E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	870	0.0	23.816093	37
GGTATCA	735	0.0	22.149658	1
CATATAA	240	0.0	20.8125	37
ACATATA	255	0.0	20.313725	36
TATACCG	70	1.2195137E-4	18.5	5
CTCTATA	50	0.0070358627	18.5	2
TACTTAC	715	0.0	17.853148	31
TTACATA	425	0.0	17.847057	34
TACATAT	385	0.0	17.779222	35
CCTATAC	75	2.0677989E-4	17.266666	3
GTACTTA	770	0.0	16.818184	30
GTCTTAG	215	0.0	16.348839	1
GTATATA	125	1.6585909E-7	16.279999	1
GTATCAA	1025	0.0	16.243902	2
GTATAAT	160	6.2937033E-10	16.1875	1
ACTTACA	625	0.0	15.984	32
GTATAGG	70	0.0025929909	15.857142	1
CTCGTTA	1165	0.0	15.721029	22
ATTGCGA	95	7.061983E-5	15.578946	10
TCTTATA	1490	0.0	15.520134	37
>>END_MODULE