FastQCFastQC Report
Thu 9 Feb 2017
SRR2727554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803335
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC18350.020.8692131
CTTATAC23750.019.00631537
GTACTTA20450.018.81662830
ACTTACA16100.018.1552832
CTTACAT12900.017.06589133
AGTGTAC24950.016.9799627
CATATAA9000.016.44444337
ACATATA9050.016.3535936
TACATAT11150.016.2600935
TTACATA11950.016.2552334
GTGTACT25850.015.45841328
CAGTGTA27150.015.39963126
GTCTTAG6200.015.2177421
TCTTATA39900.015.2080237
ACAGCTA32500.014.74307619
TGTACTT27700.014.559566529
CTAATAG1551.2123928E-714.3225813
CAGCTAC33750.014.30666720
ACAGTGT30950.014.22617225
CTACAGT31050.014.06119123
Produced by FastQC (version 0.11.5)