##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727553.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501302
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57880293238802	34.0	31.0	34.0	31.0	34.0
2	32.76473620897061	34.0	31.0	34.0	31.0	34.0
3	32.86145425770431	34.0	31.0	34.0	31.0	34.0
4	36.31898112438404	37.0	37.0	37.0	35.0	37.0
5	36.16906791571583	37.0	35.0	37.0	35.0	37.0
6	36.186334927949204	37.0	36.0	37.0	35.0	37.0
7	36.163913056800034	37.0	36.0	37.0	35.0	37.0
8	36.15125537699943	37.0	35.0	37.0	35.0	37.0
9	37.86671835513441	39.0	38.0	39.0	35.0	39.0
10	37.84503051351427	39.0	38.0	39.0	35.0	39.0
11	37.91752358952429	39.0	38.0	39.0	35.0	39.0
12	37.89759488763753	39.0	38.0	39.0	35.0	39.0
13	37.928356852918334	39.0	38.0	39.0	35.0	39.0
14	39.28648799508693	40.0	39.0	41.0	36.0	41.0
15	39.29751908676602	40.0	39.0	41.0	36.0	41.0
16	39.280715006041426	40.0	39.0	41.0	36.0	41.0
17	39.29093147148275	40.0	39.0	41.0	36.0	41.0
18	39.27195327788812	40.0	39.0	41.0	36.0	41.0
19	39.30189262386915	40.0	39.0	41.0	36.0	41.0
20	39.28574464031887	40.0	39.0	41.0	36.0	41.0
21	39.26081094942923	40.0	39.0	41.0	36.0	41.0
22	39.23219778565539	40.0	39.0	41.0	36.0	41.0
23	39.19423273931561	40.0	39.0	41.0	36.0	41.0
24	39.15615645619602	40.0	39.0	41.0	36.0	41.0
25	39.10167707762995	40.0	39.0	41.0	36.0	41.0
26	39.010337693548664	40.0	39.0	41.0	36.0	41.0
27	38.96829818384309	40.0	38.0	41.0	36.0	41.0
28	38.908790503176576	40.0	38.0	41.0	35.0	41.0
29	38.828191796187575	40.0	38.0	41.0	35.0	41.0
30	38.768429669713356	40.0	38.0	41.0	35.0	41.0
31	38.71749854459662	40.0	38.0	41.0	35.0	41.0
32	38.63700974221043	40.0	38.0	41.0	35.0	41.0
33	38.597553989803515	40.0	38.0	41.0	35.0	41.0
34	38.55682600835808	40.0	38.0	41.0	35.0	41.0
35	38.54894684746973	40.0	38.0	41.0	35.0	41.0
36	38.49977885861739	40.0	38.0	41.0	35.0	41.0
37	38.47998071007699	40.0	38.0	41.0	34.0	41.0
38	38.44618937428978	40.0	38.0	41.0	34.0	41.0
39	38.408087779807126	40.0	38.0	41.0	34.0	41.0
40	38.35104795703995	40.0	38.0	41.0	34.0	41.0
41	38.310718962607126	40.0	38.0	41.0	34.0	41.0
42	38.2669309705842	40.0	38.0	41.0	34.0	41.0
43	37.20215852639909	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	2.0
13	0.0
14	1.0
15	3.0
16	2.0
17	9.0
18	8.0
19	34.0
20	54.0
21	117.0
22	300.0
23	528.0
24	872.0
25	1424.0
26	2199.0
27	3440.0
28	4989.0
29	7367.0
30	10429.0
31	14640.0
32	20281.0
33	28201.0
34	40950.0
35	60588.0
36	94650.0
37	167440.0
38	401367.0
39	641404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.504971018489286	16.573947147209555	12.009642297152737	27.911439537148418
2	18.45085132771421	19.325825183740513	35.09280611096235	27.130517377582926
3	20.20279730527236	21.00643308275084	28.952535865535385	29.83823374644142
4	15.575014220989514	14.139660108359278	32.72739262320306	37.55793304744815
5	16.61944099188571	34.47047962368664	32.92002541793723	15.99005396649042
6	37.79546020720681	32.140235608824874	14.459449198096053	15.604854985872263
7	31.264262620045802	29.097343505836932	20.06911334295165	19.569280531165614
8	28.25580729260335	33.998689137828364	18.916380581655122	18.82912298791316
9	28.483010080583387	14.001779788476934	17.31730191527088	40.1979082156688
10	17.303247447881905	25.691832822443452	32.13850377872007	24.866415950954572
11	38.12177696426169	20.321294449750948	19.906121486549676	21.65080709943769
12	22.919439260055604	22.94355166382247	28.963592934666043	25.173416141455885
13	32.57059538986826	18.265212462249433	22.819392767078174	26.34479938080413
14	23.78368909120217	18.508201547723242	23.96639716725882	33.74171219381577
15	27.350526409743008	25.494470799346168	20.8816080975047	26.27339469340612
16	26.247417241834086	24.37104593213091	23.215182554875703	26.1663542711593
17	25.887729450836673	24.29897515623106	23.81466220653806	25.99863318639421
18	25.99810031559273	24.137315476832775	24.35958920989914	25.50499499767535
19	27.022544431433516	24.147839675161958	23.94461607324842	24.884999820156104
20	26.20611975471957	23.42333521170291	23.967462908861776	26.40308212471575
21	25.831111928179673	24.47448947646776	24.244289290229414	25.450109305123153
22	26.718674856890885	23.48907814683521	23.192069283861606	26.600177712412293
23	26.004627982910833	23.63468509333898	23.76304034764491	26.597646576105273
24	25.76963195945919	23.564612582944672	24.001300204755605	26.664455252840536
25	26.434388284302557	22.968263547240994	23.536636865867095	27.06071130258935
26	25.903049486379153	23.496671555756272	24.241291891971102	26.35898706589347
27	26.73033140567321	23.74492274039467	23.707888219691974	25.816857634240147
28	26.27599243856333	24.334477673379507	23.1076092618274	26.281920626229766
29	26.073368316301448	24.155100039832092	24.11473507662016	25.6567965672463
30	26.727267398564713	23.73446515091567	23.60877425061713	25.92949319990249
31	26.208850717577143	23.50359887617548	23.981583985100933	26.305966421146444
32	25.65299986278577	23.66679055912801	23.89832292237005	26.78188665571617
33	25.817257287341256	23.409680397415045	23.967063255760667	26.80599905948304
34	26.35559001453405	23.542698271233903	24.564278206516743	25.537433507715303
35	26.128120791153282	23.232500855923725	24.288784002152795	26.350594350770194
36	25.6027767897465	23.132454362946294	24.846832949000266	26.417935898306936
37	24.98924267069517	22.747921470829986	25.4037495453946	26.859086313080244
38	25.595982687027657	22.279394818630763	25.289848411578745	26.83477408276283
39	24.648338575449845	22.44098788917886	26.275392958911663	26.635280576459635
40	25.61196881107199	21.87427979180738	26.483545615738873	26.03020578138176
41	24.818923840772875	22.03081058974144	26.730531232223765	26.419734337261925
42	24.090689281703483	22.144178852755807	27.26939683021804	26.49573503532267
43	23.803605137407395	21.69217119540239	27.044059090043177	27.460164577147033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	26.0
2	30.0
3	88.5
4	147.0
5	147.0
6	183.0
7	219.0
8	201.5
9	184.0
10	244.5
11	305.0
12	305.0
13	565.5
14	826.0
15	1123.0
16	1420.0
17	1425.5
18	1431.0
19	1431.0
20	1738.5
21	2046.0
22	2334.5
23	2623.0
24	3633.0
25	4643.0
26	4643.0
27	5991.0
28	7339.0
29	9232.0
30	11125.0
31	14677.5
32	18230.0
33	18230.0
34	22714.5
35	27199.0
36	32481.0
37	37763.0
38	44765.0
39	51767.0
40	51767.0
41	59752.0
42	67737.0
43	77420.5
44	87104.0
45	98679.0
46	110254.0
47	110254.0
48	122984.5
49	135715.0
50	145585.5
51	155456.0
52	159140.0
53	162824.0
54	162824.0
55	154570.0
56	146316.0
57	134578.0
58	122840.0
59	115163.0
60	107486.0
61	107486.0
62	94473.5
63	81461.0
64	68967.0
65	56473.0
66	47217.0
67	37961.0
68	37961.0
69	31242.5
70	24524.0
71	20110.0
72	15696.0
73	12579.5
74	9463.0
75	9463.0
76	7648.5
77	5834.0
78	4629.0
79	3424.0
80	2698.5
81	1973.0
82	1973.0
83	1395.0
84	817.0
85	611.5
86	406.0
87	275.5
88	145.0
89	145.0
90	99.0
91	53.0
92	36.0
93	19.0
94	10.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1501302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.90366030373265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41282496042808	54.97210481166764
2	8.963913779411989	11.815094574206322
3	2.9688095884968115	5.869662558802745
4	1.4203033448234101	3.744127566619891
5	0.8293330849190532	2.7328042953575973
6	0.5189444921437	2.0520204916038876
7	0.3957945335411418	1.8259015941998813
8	0.2717604398432724	1.4328006169139198
9	0.20536374257246345	1.2180800096278948
>10	0.967148526798238	10.492792709544325
>50	0.030545845061195148	1.3632748199161158
>100	0.014045197578005762	1.7509299783750498
>500	9.09348287000699E-4	0.4598581138368053
>1k	3.031160956668996E-4	0.270547859327967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1562	0.1040430239885113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.660885018470634E-5	0.0
3	0.0	0.0	0.0	6.660885018470634E-5	0.0
4	0.0	0.0	0.0	6.660885018470634E-5	0.0
5	0.0	0.0	0.0	6.660885018470634E-5	0.0
6	0.0	0.0	0.0	6.660885018470634E-5	0.0
7	0.0	0.0	0.0	6.660885018470634E-5	0.0
8	0.0	0.0	0.0	6.660885018470634E-5	0.0
9	0.0	0.0	0.0	6.660885018470634E-5	6.660885018470634E-5
10	0.0	0.0	0.0	1.99826550554119E-4	6.660885018470634E-5
11	0.0	0.0	0.0	1.99826550554119E-4	6.660885018470634E-5
12	0.0	0.0	0.0	1.99826550554119E-4	6.660885018470634E-5
13	0.0	0.0	0.0	1.99826550554119E-4	6.660885018470634E-5
14	6.660885018470634E-5	0.0	0.0	2.6643540073882535E-4	1.99826550554119E-4
15	6.660885018470634E-5	0.0	0.0	2.6643540073882535E-4	3.330442509235317E-4
16	6.660885018470634E-5	0.0	0.0	2.6643540073882535E-4	3.330442509235317E-4
17	6.660885018470634E-5	0.0	0.0	3.99653101108238E-4	3.330442509235317E-4
18	6.660885018470634E-5	0.0	0.0	5.328708014776507E-4	3.330442509235317E-4
19	6.660885018470634E-5	0.0	0.0	7.326973520317698E-4	3.330442509235317E-4
20	6.660885018470634E-5	0.0	0.0	9.325239025858888E-4	3.99653101108238E-4
21	6.660885018470634E-5	0.0	0.0	0.0013321770036941267	3.99653101108238E-4
22	6.660885018470634E-5	0.0	0.0	0.0017318301048023649	3.99653101108238E-4
23	6.660885018470634E-5	0.0	0.0	0.0023979186066494284	4.662619512929444E-4
24	6.660885018470634E-5	0.0	0.0	0.0037300956103435553	5.328708014776507E-4
25	6.660885018470634E-5	0.0	0.0	0.0046626195129294435	5.328708014776507E-4
26	6.660885018470634E-5	0.0	0.0	0.006993929269394166	5.328708014776507E-4
27	6.660885018470634E-5	0.0	0.0	0.018650478051717774	5.328708014776507E-4
28	6.660885018470634E-5	0.0	0.0	0.05628447840607686	5.994796516623571E-4
29	1.3321770036941267E-4	0.0	0.0	0.11683192322397493	5.994796516623571E-4
30	1.3321770036941267E-4	0.0	0.0	0.19463106023971194	5.994796516623571E-4
31	1.3321770036941267E-4	0.0	0.0	0.5719035876858887	5.994796516623571E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	950	0.0	20.447369	37
TATACCG	75	9.26887E-6	19.733334	5
CTATACT	140	9.458745E-11	18.5	4
CTAGAGT	135	1.1532393E-9	17.814816	4
CGACTTA	140	1.869921E-9	17.178572	19
TCTTATA	1650	0.0	16.369698	37
TAGTACA	125	1.6585909E-7	16.279999	4
TACTATA	105	9.346826E-6	15.857144	2
ACGGACT	85	5.36551E-4	15.235294	30
GTATTAA	135	3.9742008E-7	15.074075	1
GTATAGA	135	3.9742008E-7	15.074075	1
TAGTACT	135	3.9742008E-7	15.074075	4
ATATGTA	265	0.0	14.6603775	1
CTAATAC	115	2.2106253E-5	14.478261	3
CTATAAT	90	8.2760985E-4	14.388887	1
TACGGAC	90	8.2760985E-4	14.388887	29
TATAGCA	220	1.8189894E-11	14.295454	4
TATAGTA	130	4.4458575E-6	14.230769	2
TTATAGC	250	0.0	14.059999	3
TAATACC	120	3.301681E-5	13.875001	4
>>END_MODULE