FastQCFastQC Report
Thu 9 Feb 2017
SRR2727552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781825
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT34960.19620333085460132No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG28950.16247386808468844No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT21860.12268320401835196No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG21470.12049443688353233No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG18730.10511694470556872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1500.020.9666676
CTTATAC8900.019.33145937
TAAACCG500.00703636518.55
TCTTATA16100.017.3509337
TCGTTAG650.001580395717.07692323
TACTTAC5000.017.0231
CATATAA2200.016.81818237
TATACCG1005.881444E-616.6500025
TATACTG1403.4762706E-815.8571435
ACCGTCG957.0631606E-515.5789488
TTAATAC1508.108327E-814.83
ACATATA2400.014.64583336
TACCGTG1651.6465492E-814.5757577
GTACTTA6250.014.50430
TTATACT2059.276846E-1114.4390254
TTCGTTA908.277164E-414.38888922
AGGGCGT7350.014.34693910
AGTGTAC7300.014.19178127
TTTATAC1702.438901E-814.14705853
TAGTATC800.006300415413.8757
Produced by FastQC (version 0.11.5)