##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727552.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1781825
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54546546378011	34.0	31.0	34.0	31.0	34.0
2	32.72991960489947	34.0	31.0	34.0	31.0	34.0
3	32.822301853436784	34.0	31.0	34.0	31.0	34.0
4	36.29819258344675	37.0	37.0	37.0	35.0	37.0
5	36.139182579658495	37.0	35.0	37.0	35.0	37.0
6	36.161186985253885	37.0	36.0	37.0	35.0	37.0
7	36.1348201983921	37.0	35.0	37.0	35.0	37.0
8	36.12204733910457	37.0	35.0	37.0	35.0	37.0
9	37.832677732100514	39.0	38.0	39.0	35.0	39.0
10	37.806730739550744	39.0	37.0	39.0	35.0	39.0
11	37.87911663603328	39.0	38.0	39.0	35.0	39.0
12	37.86154588694176	39.0	38.0	39.0	35.0	39.0
13	37.88795701036858	39.0	38.0	39.0	35.0	39.0
14	39.23359701429714	40.0	39.0	41.0	36.0	41.0
15	39.24055055911776	40.0	39.0	41.0	36.0	41.0
16	39.218857631922326	40.0	39.0	41.0	36.0	41.0
17	39.23391073758646	40.0	39.0	41.0	36.0	41.0
18	39.21333576529682	40.0	39.0	41.0	36.0	41.0
19	39.24957894293772	40.0	39.0	41.0	36.0	41.0
20	39.235172926634206	40.0	39.0	41.0	36.0	41.0
21	39.21014633872574	40.0	39.0	41.0	36.0	41.0
22	39.18740560941731	40.0	39.0	41.0	36.0	41.0
23	39.14463541593591	40.0	39.0	41.0	36.0	41.0
24	39.107394946192805	40.0	39.0	41.0	36.0	41.0
25	39.05044771512354	40.0	39.0	41.0	36.0	41.0
26	38.95424522610245	40.0	38.0	41.0	36.0	41.0
27	38.91113549310398	40.0	38.0	41.0	35.0	41.0
28	38.84436574860045	40.0	38.0	41.0	35.0	41.0
29	38.763557588427595	40.0	38.0	41.0	35.0	41.0
30	38.70582969708024	40.0	38.0	41.0	35.0	41.0
31	38.649152975179945	40.0	38.0	41.0	35.0	41.0
32	38.56869950752739	40.0	38.0	41.0	35.0	41.0
33	38.52349136419121	40.0	38.0	41.0	35.0	41.0
34	38.481569177668966	40.0	38.0	41.0	34.0	41.0
35	38.47455502083538	40.0	38.0	41.0	34.0	41.0
36	38.41455979122529	40.0	38.0	41.0	34.0	41.0
37	38.40320682446368	40.0	38.0	41.0	34.0	41.0
38	38.37196020933593	40.0	38.0	41.0	34.0	41.0
39	38.33320836782512	40.0	38.0	41.0	34.0	41.0
40	38.27338992325284	40.0	38.0	41.0	34.0	41.0
41	38.23002539531099	40.0	38.0	41.0	34.0	41.0
42	38.19164508299075	40.0	38.0	41.0	34.0	41.0
43	37.11265247709511	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	2.0
14	1.0
15	2.0
16	4.0
17	5.0
18	22.0
19	29.0
20	74.0
21	164.0
22	307.0
23	591.0
24	1040.0
25	1654.0
26	2635.0
27	3941.0
28	6046.0
29	8779.0
30	12419.0
31	17706.0
32	24506.0
33	34864.0
34	50777.0
35	75477.0
36	118086.0
37	207709.0
38	499024.0
39	715959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.790215088462666	16.205912477375726	12.004938756611901	27.998933677549704
2	18.448837568223592	19.224951945336944	35.1443604169882	27.181850069451265
3	20.579967168492978	21.124465084955034	28.852384493426687	29.443183253125305
4	15.834214920095969	13.963492486635893	32.3419527731399	37.86033982012824
5	16.915353640228417	34.40253672498702	32.6605025746075	16.021607060177065
6	38.34955733587754	31.58071078809647	14.227098620796093	15.84263325522989
7	31.24319167146044	29.135689531800264	19.973734794382164	19.647384002357136
8	28.380508748053263	34.110925595947975	18.952197886997883	18.556367769000882
9	28.88768537875493	13.97780365636356	17.029786875815525	40.10472408906598
10	17.370112104162867	25.538085951201715	32.06689770319757	25.024904241437852
11	38.15554277215776	20.2365552172632	19.60433824870568	22.00356376187336
12	22.977172281228516	22.76660165841202	28.794803081110658	25.461422979248805
13	32.880108877134404	18.0594615071626	22.714239613879027	26.34619000182397
14	23.944775721521474	18.489526188037544	23.86738316052362	33.69831492991736
15	28.028285606049973	25.224306539643344	20.763823607817827	25.98358424648885
16	26.36572053933467	24.16802996927308	23.436869501774865	26.029379989617386
17	26.081405300745025	24.093050664346947	23.73622549913712	26.08931853577091
18	25.958890463429352	24.094958820310637	24.482707336579068	25.463443379680946
19	27.24650288327978	24.132841328413285	23.833990431159062	24.786665357147868
20	26.078150211159905	23.180110280190256	23.945842043971773	26.79589746467807
21	25.61867747955046	24.5529723738302	24.430962636622564	25.397387509996772
22	26.694652954134103	23.369971798577303	22.898994008951494	27.0363812383371
23	25.964053708978152	23.43428787900046	23.92647987316375	26.67517853885763
24	25.49481570861336	23.42177262076803	24.07761704993476	27.005794620683847
25	26.510403659169672	22.78815259635486	23.344043326364822	27.357400418110643
26	25.96130371950107	23.293196582155936	24.290937662228334	26.45456203611466
27	27.113324821461145	23.63290446592679	23.522904886843545	25.73086582576852
28	26.212226228726166	24.492640972037098	22.803249477361696	26.491883321875044
29	26.032747323670957	24.134861728845426	24.228529737768863	25.603861209714758
30	27.061972977144222	23.34847698286869	23.58003732128576	26.009512718701334
31	26.218624163427943	23.384619701710324	24.02738765030236	26.36936848455937
32	25.755784097764934	23.446073548187954	23.896903455726573	26.90123889832054
33	25.98229343510165	23.209237719753624	23.765128449763584	27.043340395381136
34	26.580500329718127	23.555062927055125	24.493987905658525	25.370448837568222
35	26.34742469097695	23.293701682263972	24.17931053835253	26.17956308840655
36	25.81561039945	23.1836459809465	24.668359687399153	26.33238393220434
37	24.93005766559567	22.901800120662806	25.36539783648787	26.802744377253656
38	26.0865124240596	22.207848694456526	24.913782217670086	26.791856663813785
39	24.675206599974743	22.63718378628653	25.98010466796683	26.70750494577189
40	26.101104204958396	21.798493118011027	26.235573078164244	25.86482959886633
41	25.284188963562638	22.08056346723163	26.149986670969373	26.48526089823636
42	24.517727610736184	22.237088378488348	26.946754030277948	26.298429980497524
43	24.2341980834257	21.661105888625425	26.50136797945926	27.60332804848961
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	33.5
2	43.0
3	109.0
4	175.0
5	175.0
6	229.5
7	284.0
8	251.0
9	218.0
10	286.5
11	355.0
12	355.0
13	643.5
14	932.0
15	1271.5
16	1611.0
17	1546.0
18	1481.0
19	1481.0
20	1885.5
21	2290.0
22	2568.0
23	2846.0
24	3769.0
25	4692.0
26	4692.0
27	6169.5
28	7647.0
29	9675.0
30	11703.0
31	15397.5
32	19092.0
33	19092.0
34	24149.0
35	29206.0
36	35287.0
37	41368.0
38	49476.0
39	57584.0
40	57584.0
41	66581.0
42	75578.0
43	86117.0
44	96656.0
45	112204.0
46	127752.0
47	127752.0
48	146464.5
49	165177.0
50	179194.5
51	193212.0
52	200245.5
53	207279.0
54	207279.0
55	194313.5
56	181348.0
57	164217.5
58	147087.0
59	137624.0
60	128161.0
61	128161.0
62	112248.5
63	96336.0
64	81239.0
65	66142.0
66	55135.5
67	44129.0
68	44129.0
69	36218.5
70	28308.0
71	22994.0
72	17680.0
73	14262.5
74	10845.0
75	10845.0
76	8721.5
77	6598.0
78	5304.5
79	4011.0
80	3119.5
81	2228.0
82	2228.0
83	1603.5
84	979.0
85	738.0
86	497.0
87	347.0
88	197.0
89	197.0
90	123.5
91	50.0
92	33.0
93	16.0
94	10.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1781825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.80397774925967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.76312362206036	51.150902507970464
2	9.099342895055921	11.247511716378405
3	3.055235128689897	5.664770517369208
4	1.4750040879288269	3.6464447932168116
5	0.8817958968402293	2.7249246993851006
6	0.5618009398451832	2.0832919671422925
7	0.41302045278372634	1.7868414811684208
8	0.3016692083640653	1.4915485633095578
9	0.2251207635414363	1.252202279473015
>10	1.1504711409279362	12.017894155943832
>50	0.04487945430677557	1.885446918467312
>100	0.025083483607415292	2.942422778925052
>500	0.002544261298663004	1.0210285236674006
>1k	9.086647495225011E-4	1.0847690975831643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3496	0.19620333085460132	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2895	0.16247386808468844	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	2186	0.12268320401835196	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	2147	0.12049443688353233	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1873	0.10511694470556872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1224446845228909E-4	0.0
3	0.0	0.0	0.0	1.1224446845228909E-4	0.0
4	0.0	0.0	0.0	1.1224446845228909E-4	0.0
5	0.0	5.6122234226144545E-5	0.0	1.1224446845228909E-4	0.0
6	0.0	5.6122234226144545E-5	0.0	1.1224446845228909E-4	1.6836670267843362E-4
7	0.0	5.6122234226144545E-5	0.0	1.1224446845228909E-4	1.6836670267843362E-4
8	0.0	5.6122234226144545E-5	0.0	1.1224446845228909E-4	1.6836670267843362E-4
9	0.0	5.6122234226144545E-5	0.0	2.806111711307227E-4	1.6836670267843362E-4
10	0.0	5.6122234226144545E-5	0.0	3.3673340535686724E-4	1.6836670267843362E-4
11	0.0	5.6122234226144545E-5	0.0	3.928556395830118E-4	1.6836670267843362E-4
12	0.0	5.6122234226144545E-5	0.0	3.928556395830118E-4	1.6836670267843362E-4
13	0.0	5.6122234226144545E-5	0.0	3.928556395830118E-4	1.6836670267843362E-4
14	0.0	5.6122234226144545E-5	0.0	5.612223422614454E-4	2.2448893690457818E-4
15	0.0	5.6122234226144545E-5	0.0	6.1734457648759E-4	2.2448893690457818E-4
16	0.0	5.6122234226144545E-5	0.0	6.1734457648759E-4	3.3673340535686724E-4
17	0.0	5.6122234226144545E-5	0.0	7.857112791660235E-4	3.3673340535686724E-4
18	0.0	5.6122234226144545E-5	0.0	7.857112791660235E-4	3.928556395830118E-4
19	0.0	5.6122234226144545E-5	0.0	8.979557476183127E-4	3.928556395830118E-4
20	0.0	5.6122234226144545E-5	0.0	0.0010663224502967463	3.928556395830118E-4
21	0.0	5.6122234226144545E-5	0.0	0.0016275447925581917	3.928556395830118E-4
22	0.0	5.6122234226144545E-5	0.0	0.0023010116032719263	3.928556395830118E-4
23	0.0	5.6122234226144545E-5	0.0	0.002693867242854938	3.928556395830118E-4
24	0.0	5.6122234226144545E-5	0.0	0.003423456287794817	3.928556395830118E-4
25	0.0	5.6122234226144545E-5	0.0	0.004826512143448431	4.4897787380915636E-4
26	0.0	5.6122234226144545E-5	0.0	0.007408134917851079	5.051001080353008E-4
27	0.0	5.6122234226144545E-5	0.0	0.01655605909671264	5.051001080353008E-4
28	0.0	5.6122234226144545E-5	0.0	0.05045388856930395	5.051001080353008E-4
29	0.0	5.6122234226144545E-5	0.0	0.10158124394932162	5.051001080353008E-4
30	0.0	5.6122234226144545E-5	0.0	0.16494324639063881	5.051001080353008E-4
31	0.0	5.6122234226144545E-5	0.0	0.4646920993924768	5.051001080353008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	150	0.0	20.966667	6
CTTATAC	890	0.0	19.331459	37
TAAACCG	50	0.007036365	18.5	5
TCTTATA	1610	0.0	17.35093	37
TCGTTAG	65	0.0015803957	17.076923	23
TACTTAC	500	0.0	17.02	31
CATATAA	220	0.0	16.818182	37
TATACCG	100	5.881444E-6	16.650002	5
TATACTG	140	3.4762706E-8	15.857143	5
ACCGTCG	95	7.0631606E-5	15.578948	8
TTAATAC	150	8.108327E-8	14.8	3
ACATATA	240	0.0	14.645833	36
TACCGTG	165	1.6465492E-8	14.575757	7
GTACTTA	625	0.0	14.504	30
TTATACT	205	9.276846E-11	14.439025	4
TTCGTTA	90	8.277164E-4	14.388889	22
AGGGCGT	735	0.0	14.346939	10
AGTGTAC	730	0.0	14.191781	27
TTTATAC	170	2.438901E-8	14.1470585	3
TAGTATC	80	0.0063004154	13.875	7
>>END_MODULE