FastQCFastQC Report
Thu 9 Feb 2017
SRR2727551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053826
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT26660.2529829402576896No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT17680.1677696317987979No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG16020.1520175057362411No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG15270.1449005813103871No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG14910.1414844575859772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGT358.8689046E-426.428574
ATACCGT400.001931081223.1256
CATATAA502.7018954E-422.237
TACTTAC2950.021.94915231
GTATTGA806.9589805E-720.81251
GTAGTAT555.142703E-420.1818186
CTATCGG759.264573E-619.7333341
CTTATAC5900.019.1271237
GTTCTAG1103.8506187E-818.51
TATACCG609.236122E-418.55
TATCGGA1002.874367E-718.52
TTCGACT500.007034502518.54
TCTATAC701.2190753E-418.53
TCGTTAC2750.018.16363523
TCTTATA10750.017.20930137
TATACTC650.001579816217.0769235
CGATAGC650.001579816217.0769234
CTCGTTA3050.016.98360622
ATCTCGT3650.016.72602820
TACATAT1005.8768564E-616.65000235
Produced by FastQC (version 0.11.5)