##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727551.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1053826
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69416583003266	34.0	31.0	34.0	31.0	34.0
2	32.8725282921469	34.0	31.0	34.0	31.0	34.0
3	32.97095630587972	34.0	31.0	34.0	31.0	34.0
4	36.39838929766394	37.0	37.0	37.0	35.0	37.0
5	36.2593170029967	37.0	37.0	37.0	35.0	37.0
6	36.28259978402507	37.0	37.0	37.0	35.0	37.0
7	36.26101557562634	37.0	36.0	37.0	35.0	37.0
8	36.24981543442656	37.0	36.0	37.0	35.0	37.0
9	37.98569593082729	39.0	38.0	39.0	35.0	39.0
10	37.97512587467001	39.0	38.0	39.0	35.0	39.0
11	38.04205153412423	39.0	38.0	39.0	35.0	39.0
12	38.02597867200088	39.0	38.0	39.0	35.0	39.0
13	38.05097236166122	39.0	38.0	39.0	35.0	39.0
14	39.42695947907909	40.0	39.0	41.0	37.0	41.0
15	39.447775059639824	40.0	39.0	41.0	37.0	41.0
16	39.42593179519199	40.0	39.0	41.0	37.0	41.0
17	39.44374308472177	40.0	39.0	41.0	37.0	41.0
18	39.41995357867428	40.0	39.0	41.0	37.0	41.0
19	39.45593295287837	40.0	39.0	41.0	37.0	41.0
20	39.43794895931586	40.0	39.0	41.0	37.0	41.0
21	39.414316974529	40.0	39.0	41.0	37.0	41.0
22	39.39068688758865	40.0	39.0	41.0	37.0	41.0
23	39.353224346334216	40.0	39.0	41.0	36.0	41.0
24	39.318788870268904	40.0	39.0	41.0	36.0	41.0
25	39.26774628828668	40.0	39.0	41.0	36.0	41.0
26	39.183386061835634	40.0	39.0	41.0	36.0	41.0
27	39.1382543228199	40.0	39.0	41.0	36.0	41.0
28	39.080417450319125	40.0	39.0	41.0	36.0	41.0
29	38.98823999407872	40.0	38.0	41.0	36.0	41.0
30	38.93244425550328	40.0	38.0	41.0	35.0	41.0
31	38.8906309011165	40.0	38.0	41.0	35.0	41.0
32	38.81503113417205	40.0	38.0	41.0	35.0	41.0
33	38.771847534602486	40.0	38.0	41.0	35.0	41.0
34	38.74002539318635	40.0	38.0	41.0	35.0	41.0
35	38.726549734016814	40.0	38.0	41.0	35.0	41.0
36	38.68057630007231	40.0	38.0	41.0	35.0	41.0
37	38.67108232288822	40.0	38.0	41.0	35.0	41.0
38	38.62734360321343	40.0	38.0	41.0	35.0	41.0
39	38.59571314429517	40.0	38.0	41.0	35.0	41.0
40	38.53684763898404	40.0	38.0	41.0	35.0	41.0
41	38.493201913788425	40.0	38.0	41.0	35.0	41.0
42	38.461523059784064	40.0	38.0	41.0	35.0	41.0
43	37.3809329054322	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	3.0
17	1.0
18	12.0
19	23.0
20	37.0
21	79.0
22	151.0
23	283.0
24	493.0
25	780.0
26	1305.0
27	1945.0
28	2889.0
29	4232.0
30	5942.0
31	8580.0
32	12106.0
33	16986.0
34	25633.0
35	38995.0
36	61899.0
37	110718.0
38	271977.0
39	488755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.181582158724495	15.927107511107147	11.721764314032868	28.169546016135495
2	18.47496645556287	18.95483694651679	34.89152858251742	27.678668015402923
3	20.597423104003887	20.834843702850375	28.661183155473484	29.906550037672254
4	15.787331115383374	13.773336395192375	32.11801568759928	38.32131680182497
5	17.1284443541913	34.135236746863335	32.505271268691416	16.23104763025395
6	38.68409016289217	31.381935917314625	14.080787530389266	15.853186389403943
7	31.53461766933061	28.90932658712159	19.70040594936925	19.855649794178547
8	28.662701432684333	33.97221173134844	18.775015989356877	18.590070846610352
9	28.93238542226136	13.887586755308751	16.75542262195087	40.42460520047902
10	17.40230360609816	25.398405429359304	31.798228550064245	25.401062414478293
11	38.54355462856297	20.02266028737192	19.35328982203893	22.08049526202618
12	23.284204413252283	22.634571551660333	28.5318449155743	25.549379119513087
13	33.15139311423328	17.80787340604616	22.557424090884073	26.483309388836485
14	24.222689514208227	18.152237655931813	23.67231402527552	33.952758804584434
15	28.14923905844039	24.876023176501626	20.61364969169483	26.361088073363153
16	26.8053739421878	23.827273193107782	23.100397978413895	26.266954886290527
17	26.227289894157103	23.989349285365897	23.51384384139317	26.269516979083836
18	26.216472169029803	23.782484015387737	24.32099796361069	25.680045851971766
19	27.397502054418847	24.05349649752426	23.282021889761687	25.266979558295205
20	26.365073551041636	23.09669717771245	23.511186856274186	27.02704241497173
21	25.895451431260945	24.476431593071343	23.867792216172308	25.7603247594954
22	26.86534589201633	23.216261508066797	22.578300402533248	27.34009219738363
23	26.320948619601335	23.18978654920262	23.328993590972324	27.160271240223715
24	25.843545329115052	23.243210928559364	23.537092461184294	27.376151281141286
25	26.844374688041476	22.66246989540968	22.90216791007244	27.5909875064764
26	26.337270099617964	22.9079563419388	23.905084900163782	26.84968865827945
27	27.291412434310786	23.51773442674597	23.209998614572044	25.980854524371193
28	26.685619827182094	24.070102654517918	22.45342210194093	26.790855416359054
29	26.247786636503562	23.86712796989256	23.908121454585483	25.976963939018393
30	27.201264724916633	23.2746202883588	23.241218189720126	26.28289679700444
31	26.46081990765079	23.018790578330766	23.70638037019394	26.814009143824503
32	25.882640967294414	23.23087492622122	23.58112250029891	27.305361606185464
33	26.414892022022613	22.960431798038766	23.364957782404304	27.259718397534314
34	26.82435240732341	23.290846876049745	24.119161986893474	25.76563872973337
35	26.463002431141387	23.21616661574112	23.863047599888407	26.457783353229093
36	26.134959661272354	22.938037209178745	24.2210763446717	26.7059267848772
37	25.159751230279003	22.46547342730204	24.988565474755795	27.386209867663165
38	26.08637479052519	22.00771284823111	24.671814891642455	27.23409746960124
39	25.0771949069391	22.163146477691765	25.725309491320196	27.03434912404894
40	26.261640916052553	21.42431482996244	26.143499970583377	26.170544283401625
41	25.450121746853842	21.620457267138978	26.13571879987778	26.793702186129398
42	24.601594570640696	21.733094457718828	26.954260001176667	26.711050970463813
43	24.24698194958181	21.261289814447544	26.68353219601718	27.808196039953465
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	8.0
3	37.0
4	66.0
5	66.0
6	87.0
7	108.0
8	100.0
9	92.0
10	115.5
11	139.0
12	139.0
13	240.0
14	341.0
15	450.5
16	560.0
17	587.0
18	614.0
19	614.0
20	769.0
21	924.0
22	1104.0
23	1284.0
24	1753.0
25	2222.0
26	2222.0
27	2961.5
28	3701.0
29	4685.5
30	5670.0
31	7677.0
32	9684.0
33	9684.0
34	12487.0
35	15290.0
36	18460.5
37	21631.0
38	26384.0
39	31137.0
40	31137.0
41	36300.5
42	41464.0
43	48330.0
44	55196.0
45	64694.0
46	74192.0
47	74192.0
48	85127.5
49	96063.0
50	104895.0
51	113727.0
52	119542.5
53	125358.0
54	125358.0
55	117198.0
56	109038.0
57	99634.5
58	90231.0
59	85084.0
60	79937.0
61	79937.0
62	70180.0
63	60423.0
64	51081.0
65	41739.0
66	34802.0
67	27865.0
68	27865.0
69	22832.5
70	17800.0
71	14492.0
72	11184.0
73	9080.5
74	6977.0
75	6977.0
76	5587.5
77	4198.0
78	3353.0
79	2508.0
80	1977.0
81	1446.0
82	1446.0
83	1009.5
84	573.0
85	428.5
86	284.0
87	192.0
88	100.0
89	100.0
90	67.5
91	35.0
92	20.0
93	5.0
94	3.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1053826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.34403426469893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.54435957103132	56.935582478385605
2	8.52190969119865	11.477995564895034
3	2.7392241075895303	5.534112064805959
4	1.3314093688955164	3.5864991263696333
5	0.7523761185947979	2.533402155529463
6	0.49865861809575607	2.014900983805681
7	0.35035167054242683	1.6515866433992592
8	0.23893930342797354	1.2872909309789395
9	0.176755708783303	1.0713098257905511
>10	0.7959280705294614	8.843263499348271
>50	0.029921745924093593	1.3821926687876118
>100	0.018191731087891773	2.2294044255995322
>500	0.001269189192339657	0.5926080885039982
>1k	7.051051068553651E-4	0.8598515438005128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2666	0.2529829402576896	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	1768	0.1677696317987979	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	1602	0.1520175057362411	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1527	0.1449005813103871	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1491	0.1414844575859772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.489232567805311E-5
2	0.0	0.0	0.0	0.0	9.489232567805311E-5
3	0.0	0.0	0.0	0.0	9.489232567805311E-5
4	0.0	0.0	0.0	0.0	9.489232567805311E-5
5	0.0	0.0	0.0	0.0	9.489232567805311E-5
6	0.0	0.0	0.0	0.0	1.8978465135610623E-4
7	0.0	0.0	0.0	0.0	1.8978465135610623E-4
8	0.0	0.0	0.0	0.0	2.8467697703415934E-4
9	0.0	0.0	0.0	9.489232567805311E-5	2.8467697703415934E-4
10	0.0	0.0	0.0	9.489232567805311E-5	2.8467697703415934E-4
11	0.0	0.0	0.0	9.489232567805311E-5	2.8467697703415934E-4
12	0.0	0.0	0.0	1.8978465135610623E-4	2.8467697703415934E-4
13	0.0	0.0	0.0	1.8978465135610623E-4	2.8467697703415934E-4
14	0.0	0.0	0.0	1.8978465135610623E-4	3.7956930271221246E-4
15	0.0	0.0	0.0	1.8978465135610623E-4	4.7446162839026557E-4
16	0.0	0.0	0.0	2.8467697703415934E-4	4.7446162839026557E-4
17	0.0	0.0	0.0	5.693539540683187E-4	4.7446162839026557E-4
18	0.0	0.0	0.0	5.693539540683187E-4	5.693539540683187E-4
19	0.0	0.0	0.0	8.54030931102478E-4	5.693539540683187E-4
20	0.0	9.489232567805311E-5	0.0	0.0011387079081366374	5.693539540683187E-4
21	0.0	9.489232567805311E-5	0.0	0.0015182772108488498	5.693539540683187E-4
22	0.0	9.489232567805311E-5	0.0	0.0018978465135610623	5.693539540683187E-4
23	0.0	9.489232567805311E-5	0.0	0.002372308141951328	7.591386054244249E-4
24	0.0	9.489232567805311E-5	0.0	0.003416123724409912	7.591386054244249E-4
25	0.0	9.489232567805311E-5	0.0	0.0037956930271221246	7.591386054244249E-4
26	0.0	9.489232567805311E-5	0.0	0.006452678146107612	7.591386054244249E-4
27	0.0	9.489232567805311E-5	0.0	0.016701049319337347	7.591386054244249E-4
28	0.0	9.489232567805311E-5	0.0	0.05313970237970975	7.591386054244249E-4
29	0.0	9.489232567805311E-5	0.0	0.10865171290137081	7.591386054244249E-4
30	0.0	9.489232567805311E-5	0.0	0.18020052646262286	7.591386054244249E-4
31	0.0	9.489232567805311E-5	0.0	0.500272340974696	7.591386054244249E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGGT	35	8.8689046E-4	26.42857	4
ATACCGT	40	0.0019310812	23.125	6
CATATAA	50	2.7018954E-4	22.2	37
TACTTAC	295	0.0	21.949152	31
GTATTGA	80	6.9589805E-7	20.8125	1
GTAGTAT	55	5.142703E-4	20.181818	6
CTATCGG	75	9.264573E-6	19.733334	1
CTTATAC	590	0.0	19.12712	37
GTTCTAG	110	3.8506187E-8	18.5	1
TATACCG	60	9.236122E-4	18.5	5
TATCGGA	100	2.874367E-7	18.5	2
TTCGACT	50	0.0070345025	18.5	4
TCTATAC	70	1.2190753E-4	18.5	3
TCGTTAC	275	0.0	18.163635	23
TCTTATA	1075	0.0	17.209301	37
TATACTC	65	0.0015798162	17.076923	5
CGATAGC	65	0.0015798162	17.076923	4
CTCGTTA	305	0.0	16.983606	22
ATCTCGT	365	0.0	16.726028	20
TACATAT	100	5.8768564E-6	16.650002	35
>>END_MODULE