FastQCFastQC Report
Thu 9 Feb 2017
SRR2727550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323234
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT32970.2491622796874929No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG31320.23669282983962023No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT21050.15907995108952763No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG20220.15280743995393103No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG20190.15258072268396974No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG17880.13512349289694792No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG16240.12272961547239566No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT16050.1212937394293073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA358.870106E-426.4285725
TTACATA1750.019.0285734
CTTATAC6300.018.7936537
ATACTAT902.1527176E-618.56
CTTACAT1950.018.0256433
TCTTATA11800.017.71610337
ATCCTAT650.00158010517.0769231
ACATATA1554.0017767E-1016.70967736
TACTTAC3250.016.50769231
TTAAGGT904.4467753E-516.4444454
CATATAA1352.2200766E-816.44444337
ATAGTAC803.38305E-416.18753
TACATAT1851.8189894E-1116.035
ACTTACA2600.015.65384632
GTACTTA3550.015.63380230
TATATAC957.060967E-515.5789483
ATAGTAG957.060967E-515.5789483
TTATACC855.3649134E-415.2352934
TATACCG855.3649134E-415.2352935
TATACTG1252.9593812E-614.7999995
Produced by FastQC (version 0.11.5)