##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727550.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1323234
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70180784351067	34.0	31.0	34.0	31.0	34.0
2	32.8790433135787	34.0	31.0	34.0	31.0	34.0
3	32.973346362019115	34.0	31.0	34.0	31.0	34.0
4	36.39866040322422	37.0	37.0	37.0	35.0	37.0
5	36.26321648325239	37.0	37.0	37.0	35.0	37.0
6	36.28753720052538	37.0	37.0	37.0	35.0	37.0
7	36.26210405718112	37.0	36.0	37.0	35.0	37.0
8	36.25139695624508	37.0	36.0	37.0	35.0	37.0
9	37.98753961884292	39.0	38.0	39.0	35.0	39.0
10	37.97984710187314	39.0	38.0	39.0	35.0	39.0
11	38.055018235625745	39.0	38.0	39.0	35.0	39.0
12	38.03448218531265	39.0	38.0	39.0	35.0	39.0
13	38.0590205511648	39.0	38.0	39.0	35.0	39.0
14	39.43346603850868	40.0	39.0	41.0	37.0	41.0
15	39.45516212551975	40.0	39.0	41.0	37.0	41.0
16	39.42950075345706	40.0	39.0	41.0	37.0	41.0
17	39.44404315487661	40.0	39.0	41.0	37.0	41.0
18	39.42239241131954	40.0	39.0	41.0	37.0	41.0
19	39.45639017739871	40.0	39.0	41.0	37.0	41.0
20	39.43744341514804	40.0	39.0	41.0	37.0	41.0
21	39.4144346351439	40.0	39.0	41.0	37.0	41.0
22	39.394082981543704	40.0	39.0	41.0	37.0	41.0
23	39.36111677904286	40.0	39.0	41.0	37.0	41.0
24	39.32665197538757	40.0	39.0	41.0	36.0	41.0
25	39.27451380481457	40.0	39.0	41.0	36.0	41.0
26	39.18924468385788	40.0	39.0	41.0	36.0	41.0
27	39.14971048204626	40.0	39.0	41.0	36.0	41.0
28	39.08414913764308	40.0	39.0	41.0	36.0	41.0
29	39.00046930474882	40.0	39.0	41.0	36.0	41.0
30	38.939540549895185	40.0	38.0	41.0	35.0	41.0
31	38.89511076650086	40.0	38.0	41.0	35.0	41.0
32	38.81851887118983	40.0	38.0	41.0	35.0	41.0
33	38.777748304532686	40.0	38.0	41.0	35.0	41.0
34	38.74500504068064	40.0	38.0	41.0	35.0	41.0
35	38.73111105065317	40.0	38.0	41.0	35.0	41.0
36	38.6744853895834	40.0	38.0	41.0	35.0	41.0
37	38.664746371390095	40.0	38.0	41.0	35.0	41.0
38	38.632080947134064	40.0	38.0	41.0	35.0	41.0
39	38.592863393776156	40.0	38.0	41.0	35.0	41.0
40	38.53413681933807	40.0	38.0	41.0	35.0	41.0
41	38.49082701925737	40.0	38.0	41.0	35.0	41.0
42	38.44875433974641	40.0	38.0	41.0	34.0	41.0
43	37.37092759103832	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	1.0
14	0.0
15	1.0
16	2.0
17	5.0
18	8.0
19	23.0
20	48.0
21	116.0
22	207.0
23	360.0
24	582.0
25	989.0
26	1514.0
27	2319.0
28	3453.0
29	5178.0
30	7410.0
31	10471.0
32	15073.0
33	21315.0
34	31895.0
35	49342.0
36	78203.0
37	140094.0
38	343566.0
39	611055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05131669833151	16.13924672431331	11.828671270538695	27.98076530681648
2	18.583485611766324	19.24655805397987	34.72787126086542	27.442085073388377
3	20.809546913093225	21.04170539753362	28.635146920348177	29.51360076902498
4	15.935201181348122	13.771184839567303	31.84863750477995	38.44497647430462
5	17.13370424278699	34.11596134923982	32.43205661281376	16.318277795159435
6	38.73373870381202	31.202493285390187	13.975457099802455	16.088310910995336
7	31.308672540155406	28.75916126701702	19.873355732999606	20.058810459827967
8	28.592070639055528	33.750568682485486	18.825997518201618	18.831363160257368
9	28.70580713615279	13.98142732124477	16.876682431074173	40.43608311152827
10	17.515722842671817	25.498362345586646	31.89050462729948	25.09541018444206
11	38.16369591470594	20.07823257262132	19.258196207171217	22.499875305501522
12	23.104001257525127	22.820075663110227	28.56327754577044	25.51264553359421
13	33.361068412691935	17.861844541479435	22.41923952981861	26.35784751601002
14	24.118032033638798	18.531038349981937	23.721881390593047	33.62904822578622
15	28.410394533393184	24.967314926913907	20.614645633349806	26.007644906343096
16	26.703893642394316	23.75180807022794	23.258849152908706	26.285449134469037
17	26.25854535176696	23.999836763565625	23.700872256909964	26.04074562775745
18	26.246982770998933	23.803197318085843	24.312631023688933	25.637188887226298
19	27.192771648854247	24.177583103215305	23.572172419995255	25.057472827935197
20	26.278420899100237	23.118208873109367	23.646384539695926	26.95698568809447
21	25.67376594011339	24.69661450658009	23.979281064422466	25.65033848888405
22	26.835162941701924	23.401000881174454	22.594945414038637	27.168890763084985
23	26.09228602046199	23.245019399441066	23.43402603016549	27.228668549931456
24	25.749036073740548	23.46002294378772	23.614719694324663	27.17622128814707
25	26.760119525344724	22.705734586626402	22.816674904060807	27.717470983968067
26	26.254086578791053	23.07074939126413	23.96537573853151	26.70978829141331
27	27.429464478693866	23.448989369982936	23.32792234782359	25.79362380349961
28	26.47800766908952	24.27779213653821	22.618448437691292	26.625751756680984
29	26.36540475834206	23.89093690156087	23.921317015735692	25.822341324361375
30	26.89486515612507	23.320289533068227	23.563859453430005	26.2209858573767
31	26.381955119049238	23.17020270035383	23.72566001175907	26.72218216883786
32	25.855366473352408	23.170656134893754	23.522219048180443	27.451758343573395
33	26.273130829467807	23.067575349484674	23.533554911678507	27.12573890936902
34	26.86735679403643	23.386642120743574	24.01374208945659	25.732258995763406
35	26.508841217804257	23.180858412042014	23.989105479454125	26.321194890699605
36	26.050645615212424	23.148513414860865	24.287314261876585	26.51352670805013
37	24.993916419922705	22.63333620508542	25.00343854526108	27.369308829730798
38	25.984595317230365	22.14098186715275	24.81171130729712	27.06271150831977
39	24.918721858718865	22.24073746593573	25.73346815453654	27.107072520808867
40	26.16226608445672	21.533152866386445	26.246604908882333	26.05797614027451
41	25.5895026881111	21.63222831335954	26.098105097057662	26.680163901471698
42	24.50201551652996	21.783977739386987	27.056968004147414	26.65703873993564
43	24.394475958144966	21.183176973989486	26.7112241674564	27.711122900409148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	16.5
2	25.0
3	63.0
4	101.0
5	101.0
6	117.0
7	133.0
8	128.0
9	123.0
10	179.0
11	235.0
12	235.0
13	384.0
14	533.0
15	777.0
16	1021.0
17	968.5
18	916.0
19	916.0
20	1197.5
21	1479.0
22	1655.5
23	1832.0
24	2487.0
25	3142.0
26	3142.0
27	4187.5
28	5233.0
29	6546.5
30	7860.0
31	10460.5
32	13061.0
33	13061.0
34	16444.5
35	19828.0
36	24094.5
37	28361.0
38	34061.0
39	39761.0
40	39761.0
41	46166.0
42	52571.0
43	60711.5
44	68852.0
45	80402.0
46	91952.0
47	91952.0
48	105774.5
49	119597.0
50	131185.5
51	142774.0
52	150002.0
53	157230.0
54	157230.0
55	148546.0
56	139862.0
57	125491.5
58	111121.0
59	104228.5
60	97336.0
61	97336.0
62	85855.0
63	74374.0
64	62840.0
65	51306.0
66	43205.0
67	35104.0
68	35104.0
69	28688.5
70	22273.0
71	18303.5
72	14334.0
73	11621.0
74	8908.0
75	8908.0
76	7216.0
77	5524.0
78	4338.0
79	3152.0
80	2497.5
81	1843.0
82	1843.0
83	1334.0
84	825.0
85	615.0
86	405.0
87	286.0
88	167.0
89	167.0
90	107.0
91	47.0
92	29.5
93	12.0
94	8.0
95	4.0
96	4.0
97	4.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1323234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.4835946160201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.75221953301494	56.34632206240906
2	8.378146328488207	11.140185682738144
3	2.707246059678527	5.399623486724548
4	1.3303237731573128	3.5377882577058024
5	0.7385553491182777	2.455090721613639
6	0.4796252977583683	1.9132328318253178
7	0.3170081766493302	1.475309017442243
8	0.24084040143257748	1.2809548492802423
9	0.18080864738206068	1.0818727934057863
>10	0.8210841302248849	9.153104781620536
>50	0.03234626936032391	1.4715695157880586
>100	0.019071557136846877	2.582368951046955
>500	0.001816317732199974	0.8348721752424274
>1k	9.08158866099987E-4	1.3277048731571852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3297	0.2491622796874929	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	3132	0.23669282983962023	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	2105	0.15907995108952763	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	2022	0.15280743995393103	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	2019	0.15258072268396974	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1788	0.13512349289694792	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	1624	0.12272961547239566	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	1605	0.1212937394293073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5114484664088135E-4	0.0
5	0.0	0.0	0.0	1.5114484664088135E-4	0.0
6	7.557242332044067E-5	0.0	0.0	1.5114484664088135E-4	0.0
7	7.557242332044067E-5	0.0	0.0	2.2671726996132205E-4	0.0
8	7.557242332044067E-5	0.0	0.0	2.2671726996132205E-4	0.0
9	7.557242332044067E-5	0.0	0.0	3.022896932817627E-4	7.557242332044067E-5
10	7.557242332044067E-5	0.0	0.0	3.778621166022034E-4	7.557242332044067E-5
11	7.557242332044067E-5	0.0	0.0	3.778621166022034E-4	7.557242332044067E-5
12	7.557242332044067E-5	0.0	0.0	3.778621166022034E-4	7.557242332044067E-5
13	7.557242332044067E-5	0.0	0.0	3.778621166022034E-4	7.557242332044067E-5
14	7.557242332044067E-5	0.0	0.0	3.778621166022034E-4	1.5114484664088135E-4
15	7.557242332044067E-5	0.0	0.0	4.534345399226441E-4	1.5114484664088135E-4
16	7.557242332044067E-5	0.0	0.0	7.557242332044068E-4	3.022896932817627E-4
17	7.557242332044067E-5	0.0	0.0	0.0010580139264861695	3.022896932817627E-4
18	7.557242332044067E-5	0.0	0.0	0.0011335863498066101	3.778621166022034E-4
19	7.557242332044067E-5	0.0	0.0	0.0012847311964474916	3.778621166022034E-4
20	7.557242332044067E-5	0.0	0.0	0.0015114484664088136	3.778621166022034E-4
21	7.557242332044067E-5	0.0	0.0	0.001889310583011017	3.778621166022034E-4
22	7.557242332044067E-5	0.0	0.0	0.003098469356138068	3.778621166022034E-4
23	7.557242332044067E-5	0.0	0.0	0.004005338435983356	5.290069632430847E-4
24	7.557242332044067E-5	0.0	0.0	0.0051389247857899665	5.290069632430847E-4
25	7.557242332044067E-5	0.0	0.0	0.006196938712276136	5.290069632430847E-4
26	7.557242332044067E-5	0.0	0.0	0.00899311837513244	5.290069632430847E-4
27	7.557242332044067E-5	0.0	0.0	0.019422112793353253	5.290069632430847E-4
28	7.557242332044067E-5	7.557242332044067E-5	0.0	0.05471443448399905	5.290069632430847E-4
29	7.557242332044067E-5	7.557242332044067E-5	0.0	0.10633039961186003	5.290069632430847E-4
30	7.557242332044067E-5	7.557242332044067E-5	0.0	0.17804862934295823	5.290069632430847E-4
31	7.557242332044067E-5	7.557242332044067E-5	0.0	0.46439254130410795	6.045793865635254E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTA	35	8.870106E-4	26.428572	5
TTACATA	175	0.0	19.02857	34
CTTATAC	630	0.0	18.79365	37
ATACTAT	90	2.1527176E-6	18.5	6
CTTACAT	195	0.0	18.02564	33
TCTTATA	1180	0.0	17.716103	37
ATCCTAT	65	0.001580105	17.076923	1
ACATATA	155	4.0017767E-10	16.709677	36
TACTTAC	325	0.0	16.507692	31
TTAAGGT	90	4.4467753E-5	16.444445	4
CATATAA	135	2.2200766E-8	16.444443	37
ATAGTAC	80	3.38305E-4	16.1875	3
TACATAT	185	1.8189894E-11	16.0	35
ACTTACA	260	0.0	15.653846	32
GTACTTA	355	0.0	15.633802	30
TATATAC	95	7.060967E-5	15.578948	3
ATAGTAG	95	7.060967E-5	15.578948	3
TTATACC	85	5.3649134E-4	15.235293	4
TATACCG	85	5.3649134E-4	15.235293	5
TATACTG	125	2.9593812E-6	14.799999	5
>>END_MODULE