##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2596397
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10589405241186	33.0	31.0	34.0	30.0	34.0
2	32.29546637128298	34.0	31.0	34.0	30.0	34.0
3	32.3932337774231	34.0	31.0	34.0	30.0	34.0
4	35.99794176314331	37.0	35.0	37.0	35.0	37.0
5	35.71055659053681	37.0	35.0	37.0	33.0	37.0
6	35.71201129873436	37.0	35.0	37.0	33.0	37.0
7	35.68639233522455	37.0	35.0	37.0	33.0	37.0
8	35.65502656180854	37.0	35.0	37.0	33.0	37.0
9	37.31184714818266	39.0	37.0	39.0	34.0	39.0
10	37.21367957211474	39.0	37.0	39.0	34.0	39.0
11	37.30653671222082	39.0	37.0	39.0	34.0	39.0
12	37.26759043397446	39.0	37.0	39.0	34.0	39.0
13	37.318059988514854	39.0	37.0	39.0	34.0	39.0
14	38.545984685700994	40.0	38.0	41.0	35.0	41.0
15	38.53616376848379	40.0	38.0	41.0	34.0	41.0
16	38.51874501472618	40.0	38.0	41.0	34.0	41.0
17	38.524897001498616	40.0	38.0	41.0	34.0	41.0
18	38.490274792337225	40.0	38.0	41.0	34.0	41.0
19	38.5335435990721	40.0	38.0	41.0	34.0	41.0
20	38.5165196231547	40.0	38.0	41.0	34.0	41.0
21	38.48338447471631	40.0	38.0	41.0	34.0	41.0
22	38.45545192048828	40.0	38.0	41.0	34.0	41.0
23	38.4041350379006	40.0	38.0	41.0	34.0	41.0
24	38.35478973361932	40.0	38.0	41.0	34.0	41.0
25	38.282111710959455	40.0	38.0	41.0	34.0	41.0
26	38.15876770771188	40.0	38.0	41.0	34.0	41.0
27	38.095560116576934	40.0	38.0	41.0	34.0	41.0
28	38.01608883387248	40.0	38.0	41.0	33.0	41.0
29	37.92162369622211	40.0	37.0	41.0	33.0	41.0
30	37.842896521602825	40.0	37.0	41.0	33.0	41.0
31	37.77083242662813	40.0	37.0	41.0	33.0	41.0
32	37.66597211443396	40.0	37.0	41.0	33.0	41.0
33	37.61406325766052	39.0	37.0	41.0	33.0	41.0
34	37.55699032158795	39.0	37.0	41.0	33.0	41.0
35	37.533897165957285	39.0	37.0	41.0	33.0	41.0
36	37.482972365166034	39.0	37.0	40.0	32.0	41.0
37	37.44919401770993	39.0	37.0	40.0	32.0	41.0
38	37.40312825812077	39.0	37.0	40.0	32.0	41.0
39	37.359919919796546	39.0	37.0	40.0	32.0	41.0
40	37.28973689308684	39.0	36.0	40.0	32.0	41.0
41	37.24685477606083	39.0	36.0	40.0	32.0	41.0
42	37.19383014230875	39.0	36.0	40.0	31.0	41.0
43	36.10340945548774	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	1.0
13	2.0
14	5.0
15	10.0
16	18.0
17	41.0
18	47.0
19	141.0
20	286.0
21	635.0
22	1147.0
23	1952.0
24	3251.0
25	5093.0
26	7875.0
27	11780.0
28	16904.0
29	23766.0
30	32818.0
31	43869.0
32	58137.0
33	78840.0
34	109532.0
35	156857.0
36	241647.0
37	421071.0
38	800258.0
39	580406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.032440724588724	17.770009748124036	12.153457271750044	27.044092255537194
2	18.299397203124176	20.310838442657268	35.54005801115931	25.849706343059246
3	20.17603625331565	22.32312701023765	29.372896363691687	28.127940372755013
4	15.112519387443445	15.112557902354688	34.21965130910258	35.55527140109929
5	15.765578222436707	35.57880401186721	33.662918267121704	14.992699498574371
6	36.20313072307509	33.97319439207486	14.983109285675495	14.84056559917455
7	30.171849682463815	29.964639459990135	20.75838171127143	19.105129146274628
8	27.280727870198586	34.95667265060005	19.38605690886255	18.37654257033882
9	28.03504240684302	14.308058436363929	18.017275478287797	39.63962367850525
10	16.30012667554307	26.695917457923425	33.33492528299794	23.66903058353557
11	37.23217212159774	20.99351524439444	20.7146672870135	21.059645346994316
12	22.100934487291426	23.971988875353038	30.026532922353553	23.90054371500198
13	31.57918453919027	19.276096837271034	23.540775929104832	25.60394269443386
14	22.89195373434802	19.487505185069924	25.02910764416998	32.591433436412075
15	26.13956956505496	26.76913430419154	21.78045191085955	25.31084421989395
16	25.231464987827362	25.384061066162072	24.118923261735397	25.265550684275173
17	24.942680183346383	25.164025378245313	24.891686440864014	25.00160799754429
18	24.88078672098296	25.197687410669477	25.365804998234093	24.55572087011347
19	25.774255631939187	25.07678910428567	25.062076408191814	24.086878855583333
20	25.139452864873903	24.662984897918154	25.095969530083416	25.101592707124524
21	24.993866500384957	25.190177002977588	25.242210648063452	24.573745848574006
22	25.463093664027497	24.693219103241915	24.441909307397903	25.40177792533268
23	25.04204865434677	24.72626489708623	24.81115176146021	25.420534687106787
24	24.653856863954164	24.757577519924727	25.00850216665633	25.58006344946478
25	25.267977123683323	24.329599826220722	24.72433915152421	25.678083898571753
26	24.89992863186947	24.70203901791598	25.076596529729468	25.32143582048508
27	25.61611340638585	25.009465039437345	24.617421757920688	24.756999796256117
28	25.084838720734925	25.436133226159175	24.280377769655413	25.19865028345049
29	25.105444198248573	25.32332305113586	24.924308570684683	24.64692417993088
30	25.576558592541897	24.99078530748572	24.627243060287004	24.80541303968538
31	25.149851890908824	24.66421737507785	24.98408371292988	25.201847021083445
32	24.745984531641348	24.92738976358392	24.97437795529728	25.352247749477446
33	25.011968508667977	24.445183074853343	24.948611479677414	25.594236936801266
34	25.270673167470154	24.700498421466364	25.468062087577515	24.560766323485968
35	25.16830053339301	24.545822537924668	25.1463085190747	25.13956840960762
36	24.80294808536599	24.40562826100939	25.501223426155555	25.290200227469068
37	24.34369628373473	24.120040194161373	25.91760813157618	25.618655390527717
38	24.73951402655295	23.749680807673094	25.950769470154217	25.56003569561974
39	24.083836177595337	23.90986432352217	26.66564473768842	25.34065476119407
40	24.753726028800678	23.354479303434722	26.914990273059164	24.976804394705432
41	24.095660255346157	23.5798685640139	26.901433024302523	25.42303815633742
42	23.686708927794943	23.739820990395536	27.2382844380116	25.335185643797924
43	23.427118426034234	23.352515042961457	27.06723971719271	26.1531268138116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	116.5
2	158.0
3	368.0
4	578.0
5	578.0
6	726.5
7	875.0
8	846.0
9	817.0
10	1097.5
11	1378.0
12	1378.0
13	2337.0
14	3296.0
15	4595.0
16	5894.0
17	5943.5
18	5993.0
19	5993.0
20	7565.0
21	9137.0
22	10431.5
23	11726.0
24	14748.0
25	17770.0
26	17770.0
27	22237.0
28	26704.0
29	31660.0
30	36616.0
31	44052.0
32	51488.0
33	51488.0
34	60446.5
35	69405.0
36	79368.5
37	89332.0
38	102082.0
39	114832.0
40	114832.0
41	127357.0
42	139882.0
43	154049.0
44	168216.0
45	184147.0
46	200078.0
47	200078.0
48	216102.0
49	232126.0
50	242002.0
51	251878.0
52	252663.0
53	253448.0
54	253448.0
55	237263.0
56	221078.0
57	202036.5
58	182995.0
59	167999.0
60	153003.0
61	153003.0
62	134073.0
63	115143.0
64	97502.0
65	79861.0
66	67616.5
67	55372.0
68	55372.0
69	45790.5
70	36209.0
71	30234.5
72	24260.0
73	19798.0
74	15336.0
75	15336.0
76	12576.5
77	9817.0
78	7781.5
79	5746.0
80	4432.0
81	3118.0
82	3118.0
83	2346.5
84	1575.0
85	1181.0
86	787.0
87	526.5
88	266.0
89	266.0
90	176.0
91	86.0
92	56.5
93	27.0
94	18.0
95	9.0
96	9.0
97	7.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2596397.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.54801172217626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76428147496658	61.21047546562143
2	7.47334788332444	10.544596681533475
3	2.2641006827812196	4.791834045271102
4	1.066164784868352	3.008632229626561
5	0.6078038563869937	2.1439676792586786
6	0.40626139466315697	1.7196560179778442
7	0.26975743418022935	1.3321595440083707
8	0.1995995403391472	1.126508056926972
9	0.1573304100219071	0.9989412849432268
>10	0.7459708927670584	9.02124109519238
>50	0.0303069355645646	1.4317690830644703
>100	0.013873219463800686	1.7416425830267368
>500	7.099717610960105E-4	0.3355835440253437
>1k	4.915189114851216E-4	0.5929926895234916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.85149112404613E-5	7.70298224809226E-5
5	0.0	0.0	0.0	3.85149112404613E-5	7.70298224809226E-5
6	0.0	0.0	0.0	3.85149112404613E-5	1.155447337213839E-4
7	0.0	0.0	0.0	3.85149112404613E-5	1.155447337213839E-4
8	0.0	0.0	0.0	3.85149112404613E-5	1.155447337213839E-4
9	0.0	0.0	0.0	3.85149112404613E-5	1.540596449618452E-4
10	0.0	0.0	0.0	3.85149112404613E-5	1.540596449618452E-4
11	0.0	0.0	0.0	3.85149112404613E-5	1.540596449618452E-4
12	0.0	0.0	0.0	3.85149112404613E-5	1.540596449618452E-4
13	0.0	0.0	0.0	3.85149112404613E-5	1.540596449618452E-4
14	0.0	0.0	0.0	7.70298224809226E-5	2.310894674427678E-4
15	3.85149112404613E-5	0.0	0.0	7.70298224809226E-5	2.696043786832291E-4
16	3.85149112404613E-5	0.0	0.0	1.155447337213839E-4	3.466342011641517E-4
17	3.85149112404613E-5	0.0	0.0	1.925745562023065E-4	3.466342011641517E-4
18	3.85149112404613E-5	0.0	0.0	2.696043786832291E-4	4.236640236450743E-4
19	3.85149112404613E-5	0.0	0.0	4.236640236450743E-4	4.236640236450743E-4
20	3.85149112404613E-5	0.0	0.0	5.777236686069195E-4	4.621789348855356E-4
21	3.85149112404613E-5	0.0	0.0	9.243578697710712E-4	4.621789348855356E-4
22	3.85149112404613E-5	0.0	0.0	0.0016946560945802973	4.621789348855356E-4
23	3.85149112404613E-5	0.0	0.0	0.0023879244969086007	5.777236686069195E-4
24	3.85149112404613E-5	0.0	0.0	0.003812976212805669	5.777236686069195E-4
25	3.85149112404613E-5	0.0	0.0	0.005238027928702737	5.777236686069195E-4
26	3.85149112404613E-5	0.0	0.0	0.0072793182244471856	6.162385798473808E-4
27	3.85149112404613E-5	0.0	0.0	0.01744725479192897	6.162385798473808E-4
28	3.85149112404613E-5	0.0	0.0	0.050030869701359226	6.162385798473808E-4
29	3.85149112404613E-5	0.0	0.0	0.09359123431432097	6.162385798473808E-4
30	3.85149112404613E-5	0.0	0.0	0.15432924934052844	6.162385798473808E-4
31	3.85149112404613E-5	0.0	0.0	0.4518184237618515	6.162385798473808E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	95	1.678145E-7	19.473684	5
CGCGCAT	50	0.0070372117	18.499998	10
CTTATAC	1510	0.0	17.274836	37
CGCAATC	130	2.5924237E-7	15.653846	22
TCTTATA	2540	0.0	15.149606	37
TACCCCG	120	3.303506E-5	13.875001	5
CGCGCAA	160	1.7859202E-7	13.875	20
TATACTG	515	0.0	13.291262	5
GTATAAG	405	0.0	13.246914	1
CGTATAC	85	0.009408899	13.058824	3
CCCTATA	170	3.7363134E-7	13.058824	2
GTATACG	120	5.1253E-4	12.333334	1
GGGTACG	105	0.0026451154	12.333333	1
TAGGCCG	150	1.9456513E-5	12.333333	5
TAAGAGT	535	0.0	12.102803	4
TTAGAGT	370	0.0	11.5	4
GTATATA	405	0.0	11.419753	1
GTTACGG	685	0.0	11.343065	25
GGACCGT	230	1.024564E-7	11.26087	6
GTATTAG	330	1.0913936E-11	11.21212	1
>>END_MODULE