FastQCFastQC Report
Thu 9 Feb 2017
SRR2727548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812037
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64900.23079354930251628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56500.20092196510927843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46150.16411590601403894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32050.11397431826110398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21450.018.54312537
ACGCGCA852.7253855E-517.41176419
TCTTATA32750.013.38778637
ATACCGT1602.6987436E-612.718756
TCGTTAC4450.012.471909523
TTAGAGT3350.012.1492544
CGCGCAA1401.3996854E-411.89285720
GTGTACG2502.1500455E-911.841
CGTTACG4250.011.75294124
GTATTAC2052.8150134E-711.7317081
TTATACT4100.011.7317084
TACGGAT7750.011.69677427
CTCTTAT47650.011.45330637
CTACACT6350.011.3622054
ACCGTGC3104.1836756E-1111.338718
CCTACAC5400.011.3055563
TTATACC2655.3641998E-911.1698114
TATACTC2502.717934E-811.0999995
ACGGATG8600.010.9709328
CCGATAG1350.001376045410.9629633
Produced by FastQC (version 0.11.5)