##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727548.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2812037
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.727554793909185	31.0	31.0	34.0	30.0	34.0
2	31.912084371578327	31.0	31.0	34.0	30.0	34.0
3	32.01008130405112	33.0	31.0	34.0	30.0	34.0
4	35.737672370598254	37.0	35.0	37.0	33.0	37.0
5	35.20911353584608	37.0	35.0	37.0	32.0	37.0
6	35.194152495148536	37.0	35.0	37.0	32.0	37.0
7	35.16701487213717	37.0	35.0	37.0	32.0	37.0
8	35.12125445006591	37.0	35.0	37.0	32.0	37.0
9	36.74266732621228	38.0	37.0	39.0	33.0	39.0
10	36.574433053334644	38.0	35.0	39.0	32.0	39.0
11	36.69751287056322	38.0	35.0	39.0	32.0	39.0
12	36.652496393184016	38.0	35.0	39.0	32.0	39.0
13	36.73275742815617	38.0	37.0	39.0	32.0	39.0
14	37.83468247395038	39.0	37.0	40.0	33.0	41.0
15	37.79852932233822	39.0	37.0	40.0	33.0	41.0
16	37.779530639177224	39.0	37.0	40.0	33.0	41.0
17	37.78061028357735	39.0	37.0	40.0	33.0	41.0
18	37.74528855772524	39.0	37.0	40.0	33.0	41.0
19	37.80016123543182	39.0	37.0	40.0	33.0	41.0
20	37.78343883810917	39.0	37.0	40.0	33.0	41.0
21	37.740935841171364	39.0	37.0	40.0	33.0	41.0
22	37.710529413375426	39.0	37.0	40.0	33.0	41.0
23	37.647412889659705	39.0	37.0	40.0	33.0	41.0
24	37.58218188452001	39.0	37.0	40.0	32.0	41.0
25	37.49417344081888	39.0	37.0	40.0	32.0	41.0
26	37.34064167718988	39.0	36.0	40.0	32.0	41.0
27	37.25466414559979	39.0	36.0	40.0	32.0	41.0
28	37.16442813519168	39.0	36.0	40.0	32.0	41.0
29	37.03705178843664	39.0	36.0	40.0	31.0	41.0
30	36.945559393421924	39.0	36.0	40.0	31.0	41.0
31	36.853292470902765	39.0	36.0	40.0	31.0	41.0
32	36.727017105393706	38.0	36.0	40.0	30.0	41.0
33	36.66132380192722	38.0	35.0	40.0	30.0	41.0
34	36.583269352430285	38.0	35.0	40.0	30.0	41.0
35	36.55690874622204	38.0	35.0	40.0	30.0	41.0
36	36.48555371070864	38.0	35.0	40.0	30.0	41.0
37	36.42913055553679	38.0	35.0	40.0	30.0	41.0
38	36.37805441393552	38.0	35.0	40.0	30.0	41.0
39	36.31446350101368	38.0	35.0	40.0	30.0	41.0
40	36.22381320018193	38.0	35.0	40.0	30.0	41.0
41	36.16977372630588	38.0	35.0	40.0	30.0	41.0
42	36.10129987621073	38.0	35.0	40.0	30.0	41.0
43	35.01185439594145	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	9.0
12	9.0
13	4.0
14	4.0
15	15.0
16	39.0
17	74.0
18	150.0
19	328.0
20	602.0
21	1218.0
22	2109.0
23	3587.0
24	5747.0
25	8994.0
26	13613.0
27	19749.0
28	27947.0
29	38784.0
30	52466.0
31	69535.0
32	92283.0
33	123366.0
34	171698.0
35	245014.0
36	366567.0
37	543002.0
38	691778.0
39	333335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7400492952262	18.459607750538133	12.627038691169426	26.17330426306624
2	18.29737659924105	20.779989736977143	35.62136629069959	25.30126737308222
3	20.2707859107117	22.8226015518288	29.353383330304688	27.55322920715481
4	15.425472708929505	15.402393353999255	33.8921216185989	35.28001231847234
5	15.727709130427517	35.69999256766536	33.51374821881789	15.058550083089234
6	35.889321513194886	34.085397880611104	14.930457885155848	15.094822721038165
7	29.78666354674565	30.088579915555876	21.14961502995871	18.975141507739764
8	27.092708950842397	35.09480849647426	19.46926729626957	18.343215256413767
9	27.46297434919953	14.675624822859728	18.73691562379869	39.124485204142054
10	16.74074701008557	26.719420832656184	33.218766324909666	23.321065832348577
11	36.39475583002642	21.445699327569304	21.07728312251937	21.082261719884908
12	21.851134960173	24.380973650062217	30.224851237732647	23.543040152032138
13	31.1520438742449	19.57801408729686	24.16771187576835	25.102230162689892
14	22.793690125698916	19.90084056504235	25.445255521175575	31.860213788083158
15	25.71193053291973	27.07208333318516	22.372891964081553	24.843094169813554
16	24.92876160591059	25.66484722640563	24.5238593944532	24.88253177323058
17	24.483781685660606	25.779532772861806	25.393229178705685	24.3434563627719
18	24.561732295841058	25.246289433602758	25.999195600911367	24.192782669644817
19	25.29227033641449	25.27104017479144	25.89183570486448	23.544853783929586
20	24.75966710253101	24.905362198292554	25.870747788880443	24.464222910295987
21	24.490004932367533	25.39749654787615	26.05364011924452	24.058858400511802
22	25.036263747596493	24.883598615523194	25.346643731928136	24.733493904952176
23	24.53104279922348	24.950134013172658	25.68067205374609	24.83815113385777
24	24.408213689933667	25.023959499821657	25.699590723735145	24.86823608650953
25	24.86674250729987	24.664931506946743	25.483839650758505	24.984486334994884
26	24.55974085689484	24.990247283375005	25.85869247097389	24.591319388756265
27	25.065601910643426	25.2886430726196	25.415206129933566	24.230548886803412
28	24.766032594876954	25.480105702734352	25.217235761833862	24.53662594055484
29	24.590430353512417	25.542764906720645	25.802647689201812	24.064157050565125
30	24.966990121395984	25.24792525845144	25.432880150581234	24.352204469571344
31	24.681752053760317	24.918377674262466	25.70787653220779	24.691993739769426
32	24.20519360164891	25.241346397646975	25.694896617647633	24.85856338305648
33	24.512017445005167	24.868449454968054	25.65325420682587	24.966278893200908
34	24.820939411536905	24.88509219473286	26.14656208293134	24.147406310798896
35	24.63001020256846	24.814502796371457	25.956486347796986	24.599000653263097
36	24.404621987548527	24.672968385551115	26.18916465181646	24.7332449750839
37	23.929094816319985	24.28655810716573	26.625467588086497	25.158879488427782
38	24.23453176469584	24.10092754825061	26.64474187217309	25.019798814880456
39	23.673621648648293	24.159354944476195	27.243987187935293	24.92303621894022
40	24.19381395052768	23.89591602101964	27.447611820185863	24.46265820826682
41	23.658685856551674	23.949080328601653	27.45227036486362	24.939963449983054
42	23.229317395183635	24.25899801460649	27.648498223885387	24.863186366324484
43	23.12956764082407	23.889443844444436	27.494872933748738	25.486115580982755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	768.0
1	821.0
2	874.0
3	1609.5
4	2345.0
5	2345.0
6	2830.5
7	3316.0
8	2993.5
9	2671.0
10	3627.5
11	4584.0
12	4584.0
13	7224.5
14	9865.0
15	12608.0
16	15351.0
17	14347.0
18	13343.0
19	13343.0
20	15305.0
21	17267.0
22	16807.0
23	16347.0
24	19406.0
25	22465.0
26	22465.0
27	27182.5
28	31900.0
29	37126.0
30	42352.0
31	49985.5
32	57619.0
33	57619.0
34	67013.0
35	76407.0
36	87027.5
37	97648.0
38	110936.0
39	124224.0
40	124224.0
41	138113.5
42	152003.0
43	166959.5
44	181916.0
45	198201.5
46	214487.0
47	214487.0
48	230490.5
49	246494.0
50	255189.5
51	263885.0
52	264311.5
53	264738.0
54	264738.0
55	250253.0
56	235768.0
57	215510.5
58	195253.0
59	180169.5
60	165086.0
61	165086.0
62	144100.0
63	123114.0
64	103504.5
65	83895.0
66	69887.0
67	55879.0
68	55879.0
69	45673.5
70	35468.0
71	29018.5
72	22569.0
73	18162.0
74	13755.0
75	13755.0
76	11096.5
77	8438.0
78	6673.0
79	4908.0
80	3853.0
81	2798.0
82	2798.0
83	2028.0
84	1258.0
85	952.5
86	647.0
87	431.0
88	215.0
89	215.0
90	148.5
91	82.0
92	53.0
93	24.0
94	16.5
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2812037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.17769643091025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.13676782941862	53.35526248003494
2	9.075040659418592	11.648304090766679
3	3.0216616147433655	5.817698454838013
4	1.376160964930919	3.532753625896201
5	0.8285614529776343	2.6587582701776262
6	0.5370035663811809	2.0678191119316547
7	0.38565763749169124	1.7325433149642653
8	0.27677684763383453	1.4210320405238825
9	0.2120033700597796	1.2245299133423815
>10	1.0983425493197587	12.119318926385944
>50	0.038492268428032855	1.6507037766037378
>100	0.012146055385081662	1.3481271945710946
>500	7.202955821598718E-4	0.3154808894136585
>1k	5.540735245264433E-4	0.6759796783163637
>5k	1.1081470490528868E-4	0.43168823223359576
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6490	0.23079354930251628	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5650	0.20092196510927843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4615	0.16411590601403894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3205	0.11397431826110398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.112281950770919E-5	0.0
3	0.0	0.0	0.0	7.112281950770919E-5	0.0
4	0.0	0.0	0.0	7.112281950770919E-5	0.0
5	0.0	0.0	0.0	7.112281950770919E-5	0.0
6	0.0	0.0	0.0	7.112281950770919E-5	0.0
7	0.0	0.0	0.0	7.112281950770919E-5	0.0
8	0.0	0.0	0.0	7.112281950770919E-5	0.0
9	0.0	0.0	0.0	7.112281950770919E-5	7.112281950770919E-5
10	3.556140975385459E-5	0.0	0.0	2.8449127803083674E-4	7.112281950770919E-5
11	7.112281950770919E-5	0.0	0.0	2.8449127803083674E-4	7.112281950770919E-5
12	7.112281950770919E-5	0.0	0.0	3.556140975385459E-4	7.112281950770919E-5
13	7.112281950770919E-5	0.0	0.0	3.556140975385459E-4	7.112281950770919E-5
14	7.112281950770919E-5	0.0	0.0	3.556140975385459E-4	1.4224563901541837E-4
15	7.112281950770919E-5	3.556140975385459E-5	0.0	3.556140975385459E-4	2.1336845852312753E-4
16	7.112281950770919E-5	3.556140975385459E-5	0.0	4.622983268001097E-4	2.1336845852312753E-4
17	7.112281950770919E-5	3.556140975385459E-5	0.0	5.689825560616735E-4	2.1336845852312753E-4
18	7.112281950770919E-5	3.556140975385459E-5	0.0	8.534738340925101E-4	2.1336845852312753E-4
19	7.112281950770919E-5	3.556140975385459E-5	0.0	0.0011735265218772014	2.1336845852312753E-4
20	7.112281950770919E-5	3.556140975385459E-5	0.0	0.0014224563901541836	2.489298682769821E-4
21	7.112281950770919E-5	3.556140975385459E-5	0.0	0.001991438946215857	2.489298682769821E-4
22	7.112281950770919E-5	3.556140975385459E-5	0.0	0.003307211107108477	2.489298682769821E-4
23	7.112281950770919E-5	3.556140975385459E-5	0.0	0.00433849198997026	3.911755072924005E-4
24	7.112281950770919E-5	3.556140975385459E-5	0.0	0.006401053755693826	3.911755072924005E-4
25	7.112281950770919E-5	3.556140975385459E-5	0.0	0.008463615521417393	3.911755072924005E-4
26	7.112281950770919E-5	3.556140975385459E-5	0.0	0.012375370594341397	4.2673691704625506E-4
27	7.112281950770919E-5	3.556140975385459E-5	0.0	0.02720447846169876	4.2673691704625506E-4
28	7.112281950770919E-5	3.556140975385459E-5	0.0	0.07076720541017063	4.2673691704625506E-4
29	7.112281950770919E-5	3.556140975385459E-5	0.0	0.13246625133310835	4.2673691704625506E-4
30	7.112281950770919E-5	3.556140975385459E-5	0.0	0.2203740562446369	4.2673691704625506E-4
31	7.112281950770919E-5	3.556140975385459E-5	0.0	0.5597010281159174	4.2673691704625506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2145	0.0	18.543125	37
ACGCGCA	85	2.7253855E-5	17.411764	19
TCTTATA	3275	0.0	13.387786	37
ATACCGT	160	2.6987436E-6	12.71875	6
TCGTTAC	445	0.0	12.4719095	23
TTAGAGT	335	0.0	12.149254	4
CGCGCAA	140	1.3996854E-4	11.892857	20
GTGTACG	250	2.1500455E-9	11.84	1
CGTTACG	425	0.0	11.752941	24
GTATTAC	205	2.8150134E-7	11.731708	1
TTATACT	410	0.0	11.731708	4
TACGGAT	775	0.0	11.696774	27
CTCTTAT	4765	0.0	11.453306	37
CTACACT	635	0.0	11.362205	4
ACCGTGC	310	4.1836756E-11	11.33871	8
CCTACAC	540	0.0	11.305556	3
TTATACC	265	5.3641998E-9	11.169811	4
TATACTC	250	2.717934E-8	11.099999	5
ACGGATG	860	0.0	10.97093	28
CCGATAG	135	0.0013760454	10.962963	3
>>END_MODULE