FastQCFastQC Report
Thu 9 Feb 2017
SRR2727547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108096
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.19648061568353622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37550.17812281793618506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29550.14017388202434805No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT24860.11792631834603358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14350.019.5958237
ATACCGT953.6092752E-617.5263166
CGACTTA1557.21775E-915.51612919
CTAATAC2251.8189894E-1214.83
TATACCG908.2780496E-414.3888895
CATTACG4450.014.13483224
TCGCACG1051.656665E-414.09523822
ATCGCAC1051.656665E-414.09523821
GCTTATA2750.013.4545461
TACGGAT9100.013.21428527
CTCTTAT36100.013.1703637
TAATACT2950.013.1694924
CGTTACG4500.013.15555624
GTATTAC1703.7351856E-713.0588241
ATTACGG4550.013.01098825
TCTTATA22250.012.97078637
GATCGCA1153.5793716E-412.86956520
ACGGATG9500.012.85263228
CCCCTAT1451.37314655E-512.7586211
TCGTTAC5450.012.55963323
Produced by FastQC (version 0.11.5)