##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108096
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19208850071344	33.0	31.0	34.0	30.0	34.0
2	32.374152790005766	34.0	31.0	34.0	31.0	34.0
3	32.46812099638726	34.0	31.0	34.0	31.0	34.0
4	36.045108002671604	37.0	35.0	37.0	35.0	37.0
5	35.79780474892984	37.0	35.0	37.0	35.0	37.0
6	35.812731488509066	37.0	35.0	37.0	35.0	37.0
7	35.7845235700841	37.0	35.0	37.0	35.0	37.0
8	35.76580098819029	37.0	35.0	37.0	35.0	37.0
9	37.41977642384408	39.0	37.0	39.0	35.0	39.0
10	37.34549754849874	39.0	37.0	39.0	34.0	39.0
11	37.438616173077506	39.0	37.0	39.0	35.0	39.0
12	37.40712709478126	39.0	37.0	39.0	35.0	39.0
13	37.450480907890345	39.0	37.0	39.0	35.0	39.0
14	38.69085373721121	40.0	38.0	41.0	35.0	41.0
15	38.692981723792464	40.0	38.0	41.0	35.0	41.0
16	38.673016788609246	40.0	38.0	41.0	35.0	41.0
17	38.68173650535839	40.0	38.0	41.0	35.0	41.0
18	38.652706043747536	40.0	38.0	41.0	35.0	41.0
19	38.71036945186557	40.0	38.0	41.0	35.0	41.0
20	38.687411768724004	40.0	38.0	41.0	35.0	41.0
21	38.66032476699353	40.0	38.0	41.0	35.0	41.0
22	38.62911461337624	40.0	38.0	41.0	35.0	41.0
23	38.57805289702177	40.0	38.0	41.0	34.0	41.0
24	38.52844604799781	40.0	38.0	41.0	34.0	41.0
25	38.46548164789459	40.0	38.0	41.0	34.0	41.0
26	38.33820566046328	40.0	38.0	41.0	34.0	41.0
27	38.278995833206835	40.0	38.0	41.0	34.0	41.0
28	38.198238125777955	40.0	38.0	41.0	34.0	41.0
29	38.099882548043354	40.0	38.0	41.0	34.0	41.0
30	38.02893796107957	40.0	38.0	41.0	33.0	41.0
31	37.94953408193934	40.0	37.0	41.0	33.0	41.0
32	37.83784894046571	40.0	37.0	41.0	33.0	41.0
33	37.78356915434591	40.0	37.0	41.0	33.0	41.0
34	37.724554289747715	40.0	37.0	41.0	33.0	41.0
35	37.69596688196363	40.0	37.0	41.0	33.0	41.0
36	37.620951322899906	40.0	37.0	41.0	33.0	41.0
37	37.58946793694405	40.0	37.0	41.0	33.0	41.0
38	37.54053657897933	40.0	37.0	41.0	33.0	41.0
39	37.490294559640546	40.0	37.0	41.0	33.0	41.0
40	37.41625191642127	39.0	37.0	41.0	32.0	41.0
41	37.3667366192052	39.0	37.0	41.0	32.0	41.0
42	37.30509331643341	39.0	37.0	41.0	32.0	41.0
43	36.23253684841677	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	3.0
13	1.0
14	5.0
15	9.0
16	14.0
17	21.0
18	36.0
19	90.0
20	223.0
21	429.0
22	790.0
23	1300.0
24	2288.0
25	3753.0
26	5773.0
27	8751.0
28	12834.0
29	18156.0
30	24824.0
31	33386.0
32	44114.0
33	59540.0
34	82855.0
35	117764.0
36	180787.0
37	319674.0
38	647327.0
39	543343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9489928352409	18.030345866601902	12.547341297550016	26.47332000060718
2	18.161459440177296	20.817315719967212	35.765306703300034	25.25591813655545
3	20.22460077719421	22.683834132790917	29.66847809587419	27.423086994140682
4	15.204620662436625	15.218282279364887	34.267130149670606	35.30996690852788
5	15.73419806308631	35.75150277786211	33.587417271319715	14.926881887731868
6	35.81113004341358	33.91434735420019	15.102111099304775	15.172411503081454
7	29.60311105376605	30.30383815537812	21.1367508880051	18.956299902850724
8	27.045495081817904	34.93370320896202	19.71153116366617	18.3092705455539
9	27.81315461914448	14.56499134764261	18.65925460700082	38.962599426212094
10	16.564236163817966	26.775962764504087	33.460999878563406	23.198801193114544
11	36.37581020978172	21.489201630286285	20.974329442302437	21.16065871762956
12	21.76584937308358	24.38755161055284	30.195588815689607	23.651010200673973
13	31.35915062691642	19.62595631318498	23.880316645921248	25.13457641397735
14	22.653759601080786	20.1590914265764	25.539017198457753	31.64813177388506
15	26.075710024590908	27.046538677555482	22.273416390904398	24.60433490694921
16	24.779232065332888	25.722595175931268	24.802950150277788	24.69522260845806
17	24.43835574850481	25.61524712347066	25.407571571693126	24.5388255563314
18	24.265261164576945	25.449789762895048	26.149330960259874	24.13561811226813
19	25.344718646589147	25.396945869637815	25.849581802726252	23.408753681046786
20	24.563587237013873	24.916037979295062	25.83416504751207	24.686209736178995
21	24.295478004796745	25.698165548438023	26.154074577248853	23.85228186951638
22	24.95308562797899	25.029173244482223	25.057208020887096	24.96053310665169
23	24.481522663104528	25.100517243996478	25.528154315552992	24.889805777346
24	24.13153860165761	25.05317594644646	25.777953186192658	25.03733226570327
25	24.77676538449862	24.636876119493607	25.35169176356295	25.234666732444822
26	24.335561568353622	24.991366617080057	25.96025038707915	24.71282142748717
27	25.09586849934728	25.418576763107563	25.34196734873554	24.143587388809618
28	24.572932162482164	25.809830292358605	24.94155863869577	24.675678906463464
29	24.4226069401014	25.79360712225629	25.82491499438356	23.958870943258752
30	25.129643052308815	25.216024317678134	25.33494679559185	24.319385834421205
31	24.58279888581924	24.95787668113786	25.801481526457998	24.657842906584897
32	24.043686815021704	25.324748019065545	25.723449026989282	24.908116138923464
33	24.375313078721273	24.84678117125596	25.616053538358784	25.161852211663987
34	24.742231852818847	25.02703861683718	26.214603130028237	24.016126400315734
35	24.552249992410214	24.833736224536267	25.927661738364854	24.686352044688668
36	24.274463781535566	24.7461690549197	26.265881629679104	24.71348553386563
37	23.64953967940739	24.411459440177296	26.778192264488904	25.16080861592641
38	24.167827271623306	24.03121110234069	26.800677009016667	25.000284617019336
39	23.346991787850268	24.27175991985185	27.541203057166275	24.84004523513161
40	24.196146665047515	23.734735040529465	27.66624480099578	24.402873493427244
41	23.475401499741945	23.953273475211756	27.5992174929415	24.9721075321048
42	23.00896164121558	24.23158148395519	27.98477868180576	24.774678193023465
43	22.805413036218464	23.84962544400255	27.645467758584047	25.699493761194937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	307.0
1	364.5
2	422.0
3	864.5
4	1307.0
5	1307.0
6	1610.0
7	1913.0
8	1768.0
9	1623.0
10	2214.5
11	2806.0
12	2806.0
13	4566.5
14	6327.0
15	8202.5
16	10078.0
17	9559.0
18	9040.0
19	9040.0
20	10660.0
21	12280.0
22	12237.5
23	12195.0
24	14848.0
25	17501.0
26	17501.0
27	21260.0
28	25019.0
29	28983.0
30	32947.0
31	38964.5
32	44982.0
33	44982.0
34	52477.0
35	59972.0
36	67982.5
37	75993.0
38	85743.0
39	95493.0
40	95493.0
41	105379.0
42	115265.0
43	125635.0
44	136005.0
45	148435.5
46	160866.0
47	160866.0
48	173841.0
49	186816.0
50	195433.0
51	204050.0
52	205142.0
53	206234.0
54	206234.0
55	190666.0
56	175098.0
57	157837.5
58	140577.0
59	129413.0
60	118249.0
61	118249.0
62	102791.0
63	87333.0
64	73477.0
65	59621.0
66	49755.5
67	39890.0
68	39890.0
69	32796.5
70	25703.0
71	21516.5
72	17330.0
73	13871.0
74	10412.0
75	10412.0
76	8474.5
77	6537.0
78	5229.5
79	3922.0
80	3039.5
81	2157.0
82	2157.0
83	1585.0
84	1013.0
85	774.5
86	536.0
87	366.5
88	197.0
89	197.0
90	124.5
91	52.0
92	36.5
93	21.0
94	13.0
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2108096.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.485300203933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.29657257859368	59.96343252194074
2	7.716315080399011	10.723409396593214
3	2.309178030331361	4.81361785985704
4	1.1189131153043317	3.1099205487615755
5	0.6663149771976447	2.3149548110477554
6	0.4222571443099032	1.7604398661377445
7	0.28614859370425033	1.3918184655531163
8	0.20859623584592615	1.159549765533167
9	0.152990332440198	0.9567521260115999
>10	0.7788756595313296	9.086998278120324
>50	0.02803473416090115	1.3263628754309789
>100	0.01402626499947006	1.818160608806899
>500	0.0010253383742739822	0.4439097330705465
>1k	7.51914807800789E-4	1.1306731431353316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4142	0.19648061568353622	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3755	0.17812281793618506	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2955	0.14017388202434805	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2486	0.11792631834603358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.7436169889796293E-5	0.0
3	0.0	0.0	0.0	1.8974467955918517E-4	0.0
4	0.0	0.0	0.0	1.8974467955918517E-4	0.0
5	0.0	0.0	0.0	2.3718084944898145E-4	0.0
6	0.0	0.0	0.0	2.3718084944898145E-4	4.7436169889796293E-5
7	0.0	0.0	0.0	2.3718084944898145E-4	4.7436169889796293E-5
8	0.0	0.0	0.0	2.3718084944898145E-4	9.487233977959259E-5
9	0.0	0.0	0.0	2.8461701933877773E-4	1.8974467955918517E-4
10	0.0	0.0	0.0	3.32053189228574E-4	1.8974467955918517E-4
11	0.0	0.0	0.0	3.7948935911837035E-4	1.8974467955918517E-4
12	0.0	0.0	0.0	4.2692552900816663E-4	1.8974467955918517E-4
13	0.0	0.0	0.0	4.743616988979629E-4	1.8974467955918517E-4
14	9.487233977959259E-5	0.0	0.0	4.743616988979629E-4	2.8461701933877773E-4
15	9.487233977959259E-5	0.0	0.0	4.743616988979629E-4	3.32053189228574E-4
16	9.487233977959259E-5	0.0	0.0	7.115425483469444E-4	3.32053189228574E-4
17	9.487233977959259E-5	0.0	0.0	9.487233977959258E-4	3.32053189228574E-4
18	9.487233977959259E-5	0.0	0.0	0.001138468077355111	3.32053189228574E-4
19	9.487233977959259E-5	0.0	0.0	0.0015653936063632775	3.32053189228574E-4
20	9.487233977959259E-5	0.0	0.0	0.0018974467955918516	3.32053189228574E-4
21	9.487233977959259E-5	0.0	0.0	0.002798734023497981	3.32053189228574E-4
22	9.487233977959259E-5	0.0	0.0	0.0034628404019551294	3.32053189228574E-4
23	9.487233977959259E-5	0.0	0.0	0.004696180819089833	3.32053189228574E-4
24	9.487233977959259E-5	0.0	0.0	0.006546191444791888	3.32053189228574E-4
25	9.487233977959259E-5	0.0	0.0	0.00882312759950211	3.32053189228574E-4
26	9.487233977959259E-5	0.0	0.0	0.012333404171347035	3.32053189228574E-4
27	9.487233977959259E-5	0.0	0.0	0.026516818968396125	3.32053189228574E-4
28	9.487233977959259E-5	0.0	0.0	0.06954142505844137	3.32053189228574E-4
29	9.487233977959259E-5	0.0	0.0	0.1290738182701357	3.32053189228574E-4
30	9.487233977959259E-5	0.0	0.0	0.2146486687513282	3.32053189228574E-4
31	9.487233977959259E-5	0.0	0.0	0.5706096876043596	3.32053189228574E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1435	0.0	19.59582	37
ATACCGT	95	3.6092752E-6	17.526316	6
CGACTTA	155	7.21775E-9	15.516129	19
CTAATAC	225	1.8189894E-12	14.8	3
TATACCG	90	8.2780496E-4	14.388889	5
CATTACG	445	0.0	14.134832	24
TCGCACG	105	1.656665E-4	14.095238	22
ATCGCAC	105	1.656665E-4	14.095238	21
GCTTATA	275	0.0	13.454546	1
TACGGAT	910	0.0	13.214285	27
CTCTTAT	3610	0.0	13.17036	37
TAATACT	295	0.0	13.169492	4
CGTTACG	450	0.0	13.155556	24
GTATTAC	170	3.7351856E-7	13.058824	1
ATTACGG	455	0.0	13.010988	25
TCTTATA	2225	0.0	12.970786	37
GATCGCA	115	3.5793716E-4	12.869565	20
ACGGATG	950	0.0	12.852632	28
CCCCTAT	145	1.37314655E-5	12.758621	1
TCGTTAC	545	0.0	12.559633	23
>>END_MODULE