FastQCFastQC Report
Thu 9 Feb 2017
SRR2727546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761325
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.18393343775180393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49030.17755968602029823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37610.13620272876246006No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT34840.12617131268503345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20300.018.59113337
TATACCG1201.0427357E-716.9583325
TCGTTAC8350.015.73053823
CGTTACG6900.015.55072424
CTCGTTA8850.015.46892622
GTGTTAT2350.014.9574471
TATTCCG908.2791975E-414.3888885
TACGGAT12500.014.35600127
TCTTATA32250.014.05426437
TTAGTAC1203.3036555E-513.8749993
TCTCGTT10500.013.74285821
GTTACGG11200.013.54464225
ATCTCGT10650.013.375586520
GTCCTAG3200.013.2968751
GTATTAG2651.8189894E-1213.2641521
GTATAGA4200.013.2142871
GTACTTA10100.013.0049530
ACGGATG14250.012.98245628
TTACGGA14550.012.84192426
TATACTG3900.012.8076935
Produced by FastQC (version 0.11.5)