##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2761325
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67950929354567	34.0	31.0	34.0	31.0	34.0
2	32.83788398685414	34.0	31.0	34.0	31.0	34.0
3	32.930131368093214	34.0	31.0	34.0	31.0	34.0
4	36.357794537042906	37.0	37.0	37.0	35.0	37.0
5	36.23125347432845	37.0	35.0	37.0	35.0	37.0
6	36.25887028872009	37.0	37.0	37.0	35.0	37.0
7	36.232516636035236	37.0	36.0	37.0	35.0	37.0
8	36.22879088843219	37.0	36.0	37.0	35.0	37.0
9	37.948795596316984	39.0	38.0	39.0	35.0	39.0
10	37.93786280137253	39.0	38.0	39.0	35.0	39.0
11	38.009969489285034	39.0	38.0	39.0	35.0	39.0
12	37.99089639937349	39.0	38.0	39.0	35.0	39.0
13	38.017264537857734	39.0	38.0	39.0	35.0	39.0
14	39.39380587218093	40.0	39.0	41.0	37.0	41.0
15	39.41595900518773	40.0	39.0	41.0	37.0	41.0
16	39.39698695372692	40.0	39.0	41.0	37.0	41.0
17	39.41067929345513	40.0	39.0	41.0	37.0	41.0
18	39.390989470634565	40.0	39.0	41.0	37.0	41.0
19	39.42995482241315	40.0	39.0	41.0	37.0	41.0
20	39.40562918164287	40.0	39.0	41.0	37.0	41.0
21	39.389016866902665	40.0	39.0	41.0	37.0	41.0
22	39.35289326681937	40.0	39.0	41.0	36.0	41.0
23	39.31433170669877	40.0	39.0	41.0	36.0	41.0
24	39.28111939014784	40.0	39.0	41.0	36.0	41.0
25	39.22251817515142	40.0	39.0	41.0	36.0	41.0
26	39.1298018161571	40.0	39.0	41.0	36.0	41.0
27	39.084244701366195	40.0	39.0	41.0	36.0	41.0
28	39.01476066743321	40.0	39.0	41.0	35.0	41.0
29	38.93506921496021	40.0	38.0	41.0	35.0	41.0
30	38.87367405140648	40.0	38.0	41.0	35.0	41.0
31	38.803754719201834	40.0	38.0	41.0	35.0	41.0
32	38.713686726480944	40.0	38.0	41.0	35.0	41.0
33	38.664945632984164	40.0	38.0	41.0	35.0	41.0
34	38.61221225317556	40.0	38.0	41.0	35.0	41.0
35	38.588564547816716	40.0	38.0	41.0	35.0	41.0
36	38.52385213620273	40.0	38.0	41.0	35.0	41.0
37	38.498517197359966	40.0	38.0	41.0	35.0	41.0
38	38.45405883045277	40.0	38.0	41.0	35.0	41.0
39	38.40210623523127	40.0	38.0	41.0	34.0	41.0
40	38.32956533548206	40.0	38.0	41.0	34.0	41.0
41	38.27307180429685	40.0	38.0	41.0	34.0	41.0
42	38.215249563162615	40.0	38.0	41.0	34.0	41.0
43	37.16951970521398	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	0.0
14	1.0
15	1.0
16	9.0
17	16.0
18	20.0
19	73.0
20	100.0
21	262.0
22	480.0
23	859.0
24	1661.0
25	2668.0
26	4176.0
27	6450.0
28	9568.0
29	13652.0
30	19296.0
31	26393.0
32	35863.0
33	49894.0
34	72112.0
35	105089.0
36	161995.0
37	285161.0
38	675736.0
39	1289784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81378504884431	17.247661901442243	12.13051705250197	26.808035997211483
2	18.22248377137787	20.251871836889897	35.53797542846279	25.987668963269446
3	20.336432690827774	22.017509710012405	29.46820819715173	28.177849402008093
4	15.289652612423385	14.721917919839209	33.55896173032873	36.42946773740867
5	16.15130417462631	35.27219722415869	33.15277991543915	15.42371868577585
6	36.503816102776746	32.98988710130101	14.889192688292757	15.61710410762949
7	29.962029098349525	29.941423048717553	20.859370048799036	19.237177804133886
8	27.40800159343793	34.361004228042695	19.631662336016223	18.599331842503148
9	28.10455125709578	14.393886992657508	18.425719536816565	39.07584221343014
10	17.01871384208668	26.35698441871203	33.014875105248386	23.609426633952904
11	36.636976813667346	21.108489583804875	20.684309163173474	21.570224439354295
12	22.056114365386183	23.866296071632277	29.75470109458322	24.322888468398325
13	31.577485446298425	19.213058948149893	23.731759209799645	25.47769639575204
14	23.07682000488896	19.850760051786732	24.969208622672088	32.10321132065223
15	26.52936543145048	26.581297022262863	21.98973318968249	24.899604356604165
16	25.151403764497115	25.27829936715164	24.485600210044094	25.084696658307156
17	24.82572677971626	25.21220790743574	24.99097353625524	24.971091776592758
18	24.667759137370645	25.07412202475261	25.819814762840306	24.43830407503644
19	25.78830814916752	25.082197857912412	25.392737182330947	23.736756810589117
20	24.884973699220485	24.430807673852225	25.471467502014434	25.212751124912856
21	24.524458366907194	25.349497071152438	25.924112518446762	24.201932043493613
22	25.392556109838576	24.494617620164234	24.655880781871023	25.45694548812617
23	24.73015671824215	24.62024571537215	25.33428698179316	25.31531058459254
24	24.42200755072293	24.593591844494945	25.423157361049498	25.56124324373263
25	25.127212479516174	24.13547843879297	25.019727848043967	25.71758123364689
26	24.645451006310378	24.532860130553267	25.630702651806654	25.190986211329708
27	25.446443283568577	25.011941730871957	25.023928729867002	24.517686255692468
28	24.88544448770065	25.42659773840457	24.510153640009776	25.177804133885
29	24.72968592976198	25.313391216173397	25.569572578381756	24.38735027568287
30	25.52999013154917	24.73196744316587	24.974459724950883	24.763582700334076
31	24.961422505500078	24.508451558581477	25.45788706508651	25.072238870831935
32	24.41193992014703	24.829348229563706	25.372782993671517	25.385928856617745
33	24.698251745086143	24.329081147637456	25.366517885435435	25.606149221840962
34	25.077091613627516	24.551184666781346	25.95677799607073	24.41494572352041
35	24.872153766760523	24.426643006527662	25.59865282065675	25.102550406055062
36	24.471874915122267	24.252016694883796	26.040976705023855	25.23513168497008
37	23.86803436755905	23.93285831982834	26.607842249644648	25.59126506296796
38	24.55574769358913	23.467248512942156	26.48319194589554	25.493811847573177
39	23.5590884810734	23.75207554344382	27.390365132680866	25.29847084280192
40	24.564439173223	23.1417163861552	27.501905787982217	24.791938652639583
41	23.783183797633384	23.450119055163736	27.44722189528578	25.319475251917105
42	23.209763428788715	23.79680044905978	27.821100377536144	25.17233574461536
43	22.992367794446505	23.30989651707061	27.435886757263273	26.26184893121961
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	489.0
1	560.5
2	632.0
3	1283.0
4	1934.0
5	1934.0
6	2333.0
7	2732.0
8	2482.5
9	2233.0
10	2973.5
11	3714.0
12	3714.0
13	5991.0
14	8268.0
15	10221.5
16	12175.0
17	11538.5
18	10902.0
19	10902.0
20	12669.0
21	14436.0
22	13978.0
23	13520.0
24	16387.0
25	19254.0
26	19254.0
27	23234.0
28	27214.0
29	31775.0
30	36336.0
31	43187.0
32	50038.0
33	50038.0
34	58862.0
35	67686.0
36	77884.5
37	88083.0
38	100073.5
39	112064.0
40	112064.0
41	125412.5
42	138761.0
43	153478.0
44	168195.0
45	186956.0
46	205717.0
47	205717.0
48	226418.0
49	247119.0
50	261077.0
51	275035.0
52	278128.5
53	281222.0
54	281222.0
55	261208.5
56	241195.0
57	219167.5
58	197140.0
59	183218.0
60	169296.0
61	169296.0
62	147810.0
63	126324.0
64	106175.5
65	86027.0
66	71944.0
67	57861.0
68	57861.0
69	47397.5
70	36934.0
71	30369.0
72	23804.0
73	19305.0
74	14806.0
75	14806.0
76	11923.0
77	9040.0
78	7208.5
79	5377.0
80	4254.5
81	3132.0
82	3132.0
83	2324.0
84	1516.0
85	1124.0
86	732.0
87	488.0
88	244.0
89	244.0
90	168.0
91	92.0
92	60.0
93	28.0
94	21.0
95	14.0
96	14.0
97	8.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2761325.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.6962072049831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50410464559519	50.9019033547625
2	9.308324865435873	11.48576679258456
3	3.1005610242343464	5.738785662085706
4	1.4624576638177906	3.6091236422167174
5	0.8440728946793796	2.603804810312444
6	0.5805425642461062	2.149036460102399
7	0.4120048350340713	1.7793394970201863
8	0.2950796983013366	1.4564238566706533
9	0.22165565928965789	1.2307782135322682
>10	1.2033641458260458	12.819671114121364
>50	0.045052555448035596	1.8767260296244699
>100	0.0200815393834237	2.289029834649055
>500	0.0018768060582952844	0.7873843450809689
>1k	7.624524610952177E-4	1.0884430651633006
>5k	5.865018931501675E-5	0.18378332207328466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5079	0.18393343775180393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4903	0.17755968602029823	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3761	0.13620272876246006	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3484	0.12617131268503345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.621449847446425E-5	0.0
5	0.0	0.0	0.0	3.621449847446425E-5	0.0
6	0.0	0.0	0.0	3.621449847446425E-5	0.0
7	0.0	0.0	0.0	7.24289969489285E-5	0.0
8	0.0	0.0	0.0	7.24289969489285E-5	0.0
9	0.0	0.0	0.0	7.24289969489285E-5	0.0
10	0.0	0.0	0.0	1.0864349542339275E-4	0.0
11	0.0	0.0	0.0	1.44857993897857E-4	3.621449847446425E-5
12	0.0	0.0	0.0	1.8107249237232125E-4	3.621449847446425E-5
13	0.0	0.0	0.0	1.8107249237232125E-4	3.621449847446425E-5
14	0.0	0.0	0.0	2.172869908467855E-4	7.24289969489285E-5
15	0.0	3.621449847446425E-5	0.0	3.621449847446425E-4	7.24289969489285E-5
16	0.0	3.621449847446425E-5	0.0	5.79431975591428E-4	1.0864349542339275E-4
17	0.0	3.621449847446425E-5	0.0	8.69147963387142E-4	1.0864349542339275E-4
18	0.0	3.621449847446425E-5	0.0	9.415769603360705E-4	1.8107249237232125E-4
19	0.0	3.621449847446425E-5	0.0	0.0013399364435551773	2.5350148932124975E-4
20	0.0	3.621449847446425E-5	0.0	0.001991797416095534	2.5350148932124975E-4
21	0.0	3.621449847446425E-5	0.0	0.003042017871854997	2.5350148932124975E-4
22	0.0	3.621449847446425E-5	0.0	0.004707884801680353	2.5350148932124975E-4
23	0.0	3.621449847446425E-5	0.0	0.006156464740658923	3.621449847446425E-4
24	0.0	3.621449847446425E-5	0.0	0.008329334649126777	3.621449847446425E-4
25	0.0	3.621449847446425E-5	0.0	0.011262709025558383	3.621449847446425E-4
26	0.0	3.621449847446425E-5	0.0	0.01575330683639195	3.621449847446425E-4
27	0.0	3.621449847446425E-5	0.0	0.034114057562945325	3.621449847446425E-4
28	0.0	3.621449847446425E-5	0.0	0.08329334649126778	3.621449847446425E-4
29	0.0	3.621449847446425E-5	0.0	0.15351325903325397	3.621449847446425E-4
30	0.0	3.621449847446425E-5	0.0	0.24473758069042942	3.621449847446425E-4
31	0.0	3.621449847446425E-5	0.0	0.6273075425746698	4.34573981693571E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2030	0.0	18.591133	37
TATACCG	120	1.0427357E-7	16.958332	5
TCGTTAC	835	0.0	15.730538	23
CGTTACG	690	0.0	15.550724	24
CTCGTTA	885	0.0	15.468926	22
GTGTTAT	235	0.0	14.957447	1
TATTCCG	90	8.2791975E-4	14.388888	5
TACGGAT	1250	0.0	14.356001	27
TCTTATA	3225	0.0	14.054264	37
TTAGTAC	120	3.3036555E-5	13.874999	3
TCTCGTT	1050	0.0	13.742858	21
GTTACGG	1120	0.0	13.544642	25
ATCTCGT	1065	0.0	13.3755865	20
GTCCTAG	320	0.0	13.296875	1
GTATTAG	265	1.8189894E-12	13.264152	1
GTATAGA	420	0.0	13.214287	1
GTACTTA	1010	0.0	13.00495	30
ACGGATG	1425	0.0	12.982456	28
TTACGGA	1455	0.0	12.841924	26
TATACTG	390	0.0	12.807693	5
>>END_MODULE