FastQCFastQC Report
Thu 9 Feb 2017
SRR2727545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248633
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.12051222775856799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37480.11537160399466484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17150.016.9358637
TACTTAC7300.014.44520631
TCTATAC3150.014.0952393
TCTTATA27300.013.55311437
TAGTACT2408.185452E-1113.1041664
CTCGTTA9750.013.09230922
GTCTTAG4950.013.0808091
TTATACT4250.013.0588244
TAATACG1000.001833732612.9500014
GGTATCA33900.012.9336291
TACGGAT14750.012.91864327
TCGTTAC9350.012.86096223
ACGGATG15200.012.65789528
GTTCTAA3250.012.5230781
CTAATAG1951.4699253E-712.3333343
GTCTAGA3950.012.1772151
GTATAGG3500.012.1571431
CTCTTAT40850.012.04651237
CGTTACG8050.011.95031124
TAATACT3750.011.8400014
Produced by FastQC (version 0.11.5)