##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727545.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3248633
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01761725624286	33.0	31.0	34.0	30.0	34.0
2	32.20795947095286	33.0	31.0	34.0	30.0	34.0
3	32.303028381476146	34.0	31.0	34.0	30.0	34.0
4	35.943493771072326	37.0	35.0	37.0	35.0	37.0
5	35.594882832255905	37.0	35.0	37.0	33.0	37.0
6	35.58620872225333	37.0	35.0	37.0	33.0	37.0
7	35.5595701330375	37.0	35.0	37.0	33.0	37.0
8	35.526105595799834	37.0	35.0	37.0	33.0	37.0
9	37.174332403814155	39.0	37.0	39.0	34.0	39.0
10	37.0627679396226	39.0	37.0	39.0	33.0	39.0
11	37.16413242123687	39.0	37.0	39.0	34.0	39.0
12	37.1251766512253	39.0	37.0	39.0	33.0	39.0
13	37.18019179143966	39.0	37.0	39.0	34.0	39.0
14	38.38107813347953	40.0	38.0	41.0	34.0	41.0
15	38.355932787729486	40.0	38.0	41.0	34.0	41.0
16	38.33918666713045	40.0	38.0	41.0	34.0	41.0
17	38.34342875911191	40.0	38.0	41.0	34.0	41.0
18	38.30766571662604	40.0	38.0	41.0	34.0	41.0
19	38.34844163683617	40.0	38.0	41.0	34.0	41.0
20	38.33245952990073	40.0	38.0	41.0	34.0	41.0
21	38.29700430919713	40.0	38.0	41.0	34.0	41.0
22	38.272712861071106	40.0	38.0	41.0	34.0	41.0
23	38.218344146599506	40.0	38.0	41.0	34.0	41.0
24	38.159540643710756	40.0	38.0	41.0	34.0	41.0
25	38.08165095903416	40.0	38.0	41.0	34.0	41.0
26	37.945415502459035	40.0	37.0	41.0	33.0	41.0
27	37.87938773016219	40.0	37.0	41.0	33.0	41.0
28	37.794906657661855	40.0	37.0	41.0	33.0	41.0
29	37.69465464396871	39.0	37.0	41.0	33.0	41.0
30	37.62152696226382	39.0	37.0	40.0	33.0	41.0
31	37.535356256000604	39.0	37.0	40.0	32.0	41.0
32	37.42419565398738	39.0	37.0	40.0	32.0	41.0
33	37.368916710505616	39.0	37.0	40.0	32.0	41.0
34	37.299547840584026	39.0	36.0	40.0	32.0	41.0
35	37.283804911173405	39.0	36.0	40.0	32.0	41.0
36	37.218944706896714	39.0	36.0	40.0	31.0	41.0
37	37.17739523054774	39.0	36.0	40.0	31.0	41.0
38	37.132361519445254	39.0	36.0	40.0	31.0	41.0
39	37.07477483606181	39.0	36.0	40.0	31.0	41.0
40	37.00079848970321	39.0	36.0	40.0	31.0	41.0
41	36.945252664736216	39.0	36.0	40.0	31.0	41.0
42	36.89008176670002	39.0	36.0	40.0	31.0	41.0
43	35.789755875779136	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	4.0
13	3.0
14	10.0
15	13.0
16	22.0
17	64.0
18	98.0
19	226.0
20	435.0
21	865.0
22	1656.0
23	2849.0
24	4686.0
25	7400.0
26	11476.0
27	16752.0
28	23885.0
29	33235.0
30	45364.0
31	60509.0
32	81269.0
33	107759.0
34	151354.0
35	215558.0
36	335469.0
37	568469.0
38	963723.0
39	615469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32991753762275	17.635079124050023	12.13488873627769	26.90011460204954
2	18.86802849075288	20.188922540650175	34.98637734702565	25.956671621571285
3	20.39362402585949	22.11782617488648	29.151246077965716	28.337303721288308
4	15.625957133354246	14.798778440039243	33.433324108940596	36.14194031766592
5	16.131246588949875	35.40122876299046	33.15637685143259	15.311147796627072
6	36.89444144660231	33.147788623707264	14.710402806349624	15.247367123340801
7	30.575198860566893	29.697106444464488	20.569359481357235	19.158335213611384
8	27.70907024585418	34.38347760427232	19.165846065098766	18.741606084774734
9	28.196906206395123	14.145303578459	18.04740640140022	39.610383813745656
10	16.981388787222194	26.144319780042867	32.856496871145495	24.017794561589444
11	37.282881753648375	20.786743223996062	20.693288530898997	21.237086491456562
12	22.237076333337747	23.60149638324797	29.594971177107414	24.56645610630687
13	31.67698536584465	19.05616916407609	23.55738552184873	25.709459948230535
14	23.281392511865757	19.362636530503753	24.467583749841857	32.888387207788625
15	26.356439770204883	26.602727978198832	21.54173770936883	25.499094542227454
16	25.51109959173597	25.211527433231147	23.737830650615198	25.539542324417685
17	25.208141393626182	24.98971105692764	24.41156018546878	25.390587363977403
18	25.11644128468805	24.951479591569747	25.11280898765727	24.81927013608493
19	26.249256225618588	24.560638274621972	24.93655023512967	24.25355526462977
20	25.36808559169349	24.176107304210724	24.945815670775985	25.5099914333198
21	25.103389641119815	24.726030918235455	25.488751730343196	24.681827710301533
22	25.82418512648243	24.033493472485194	24.43720173993184	25.70511966110053
23	25.15611951242261	24.32909472999874	25.030158839117867	25.48462691846078
24	24.999869175742536	24.34402408643882	24.93319497770293	25.722911760115718
25	25.659007958116536	23.969035591277933	24.60955115582462	25.762405294780912
26	25.224024997591293	24.359784561691026	24.98035327474664	25.435837165971044
27	25.800544413604122	24.735173225168865	24.398754799326365	25.06552756190065
28	25.301626868901472	24.98124595791522	24.088809046759053	25.628318126424254
29	25.227564948087394	25.044410987636955	24.902905314327594	24.825118749948054
30	26.03144153248459	24.408851353784808	24.3797314131821	25.179975700548507
31	25.59141029473012	24.305454017120432	24.865782007385874	25.23735368076357
32	24.982846631183023	24.634053769693285	24.860241215305024	25.52285838381867
33	25.105359700526343	24.13833757152624	24.914633324232067	25.841669403715343
34	25.535479076891725	24.263713383444667	25.443378799636644	24.75742874002696
35	25.500387393712987	24.231638353732173	24.92405267076952	25.343921581785324
36	25.13278662132657	24.084314848737915	25.357527304561643	25.425371225373873
37	24.724522591502335	23.83017718529609	25.821599423511366	25.62370079969021
38	25.199768641148445	23.47895253172642	25.64968095811377	25.671597869011364
39	24.382378680509618	23.778493908053015	26.377802601894395	25.46132480954297
40	25.277062690676356	23.2393132742295	26.351483839510344	25.1321401955838
41	24.361077413176556	23.52543362084914	26.523340740551486	25.590148225422816
42	24.137383324001203	23.689471848620634	26.69208864159171	25.481056185786453
43	23.821804432818357	23.359856284166295	26.451094968252804	26.36724431476255
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	400.5
2	508.0
3	998.5
4	1489.0
5	1489.0
6	1773.0
7	2057.0
8	1920.0
9	1783.0
10	2351.5
11	2920.0
12	2920.0
13	4718.5
14	6517.0
15	7990.0
16	9463.0
17	9051.0
18	8639.0
19	8639.0
20	10293.0
21	11947.0
22	12163.5
23	12380.0
24	15335.0
25	18290.0
26	18290.0
27	22525.5
28	26761.0
29	31844.5
30	36928.0
31	44942.0
32	52956.0
33	52956.0
34	63080.5
35	73205.0
36	85721.0
37	98237.0
38	114775.5
39	131314.0
40	131314.0
41	147192.5
42	163071.0
43	182483.0
44	201895.0
45	224664.0
46	247433.0
47	247433.0
48	269464.5
49	291496.0
50	306419.5
51	321343.0
52	320408.5
53	319474.0
54	319474.0
55	303517.0
56	287560.0
57	266553.5
58	245547.0
59	229259.5
60	212972.0
61	212972.0
62	186693.5
63	160415.0
64	134933.0
65	109451.0
66	91424.5
67	73398.0
68	73398.0
69	60136.0
70	46874.0
71	38473.5
72	30073.0
73	24131.5
74	18190.0
75	18190.0
76	14507.5
77	10825.0
78	8526.0
79	6227.0
80	4910.5
81	3594.0
82	3594.0
83	2676.5
84	1759.0
85	1334.5
86	910.0
87	600.5
88	291.0
89	291.0
90	194.0
91	97.0
92	63.5
93	30.0
94	22.0
95	14.0
96	14.0
97	10.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3248633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.007417267674555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23014237844184	45.73713204012773
2	10.036941319868747	11.443602038258435
3	3.4194987309729195	5.848103730085706
4	1.765125311261677	4.025009405953133
5	1.0378868688169633	2.958362490364441
6	0.6694725723972496	2.2898941370348074
7	0.4689282132549457	1.8712670425126834
8	0.3769296801380052	1.719023004495869
9	0.27454704465907837	1.4086096141038265
>10	1.6211192681252078	16.540326215468635
>50	0.07267813616005331	2.79129832023486
>100	0.025108977908469228	2.3944373801718006
>500	0.0011890985303790464	0.43494883976484006
>1k	4.32399464472774E-4	0.5379857414233401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3915	0.12051222775856799	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3748	0.11537160399466484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0782178226965004E-5
2	0.0	0.0	0.0	0.0	3.0782178226965004E-5
3	0.0	0.0	0.0	0.0	3.0782178226965004E-5
4	0.0	0.0	0.0	3.0782178226965004E-5	3.0782178226965004E-5
5	0.0	0.0	0.0	3.0782178226965004E-5	3.0782178226965004E-5
6	0.0	0.0	0.0	3.0782178226965004E-5	6.156435645393001E-5
7	0.0	0.0	0.0	3.0782178226965004E-5	6.156435645393001E-5
8	0.0	0.0	0.0	3.0782178226965004E-5	6.156435645393001E-5
9	0.0	0.0	0.0	3.0782178226965004E-5	1.5391089113482503E-4
10	0.0	0.0	0.0	6.156435645393001E-5	1.5391089113482503E-4
11	0.0	0.0	0.0	9.2346534680895E-5	1.5391089113482503E-4
12	0.0	0.0	0.0	9.2346534680895E-5	1.5391089113482503E-4
13	0.0	0.0	0.0	9.2346534680895E-5	1.5391089113482503E-4
14	0.0	0.0	0.0	1.2312871290786001E-4	1.8469306936179E-4
15	0.0	0.0	0.0	1.8469306936179E-4	2.4625742581572003E-4
16	0.0	0.0	0.0	4.0016831695054503E-4	2.7703960404268504E-4
17	0.0	0.0	0.0	6.772079209932301E-4	2.7703960404268504E-4
18	0.0	0.0	0.0	8.619009903550201E-4	3.3860396049661506E-4
19	0.0	0.0	0.0	9.850297032628801E-4	3.3860396049661506E-4
20	0.0	0.0	0.0	0.0013544158419864602	3.3860396049661506E-4
21	0.0	0.0	0.0	0.0018161485153909353	3.6938613872358E-4
22	0.0	0.0	0.0	0.0028935247533347103	3.6938613872358E-4
23	0.0	0.0	0.0	0.0039401188130515205	4.6173267340447505E-4
24	0.0	0.0	0.0	0.005663920793761561	4.6173267340447505E-4
25	0.0	0.0	0.0	0.007633980200287321	5.540792080853701E-4
26	0.0	0.0	0.0	0.010342811884260242	5.540792080853701E-4
27	0.0	0.0	0.0	0.022286297036322664	5.540792080853701E-4
28	0.0	0.0	0.0	0.05666999011584257	5.540792080853701E-4
29	0.0	0.0	0.0	0.10579834656607871	5.540792080853701E-4
30	0.0	0.0	0.0	0.17290349510086242	5.540792080853701E-4
31	0.0	0.0	0.0	0.43430575260424925	5.540792080853701E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1715	0.0	16.93586	37
TACTTAC	730	0.0	14.445206	31
TCTATAC	315	0.0	14.095239	3
TCTTATA	2730	0.0	13.553114	37
TAGTACT	240	8.185452E-11	13.104166	4
CTCGTTA	975	0.0	13.092309	22
GTCTTAG	495	0.0	13.080809	1
TTATACT	425	0.0	13.058824	4
TAATACG	100	0.0018337326	12.950001	4
GGTATCA	3390	0.0	12.933629	1
TACGGAT	1475	0.0	12.918643	27
TCGTTAC	935	0.0	12.860962	23
ACGGATG	1520	0.0	12.657895	28
GTTCTAA	325	0.0	12.523078	1
CTAATAG	195	1.4699253E-7	12.333334	3
GTCTAGA	395	0.0	12.177215	1
GTATAGG	350	0.0	12.157143	1
CTCTTAT	4085	0.0	12.046512	37
CGTTACG	805	0.0	11.950311	24
TAATACT	375	0.0	11.840001	4
>>END_MODULE