FastQCFastQC Report
Thu 9 Feb 2017
SRR2727544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013535
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19800.019.3409137
TACTTAC11250.014.831
TCTTATA33000.014.18333337
GTACTTA12450.013.96787130
TTACATA6900.013.67391234
ACTTACA10500.013.39047532
TCGTTAC13200.013.31439423
TACGGAT24900.013.29919627
CGTTACG11150.013.27354224
GTATAGG4200.013.2142851
ACGGATG26150.013.01720928
CGTATAC1602.6995604E-612.718753
GTACCGT3650.012.6712336
GTCTTAG5950.012.4369751
CTCGTTA15350.012.4136822
GTTACGG19250.012.39740325
GGTATCA29200.012.3544521
AGTGTAC14550.012.33333327
TACATAT6950.012.24460435
TACCGTC3200.012.1406257
Produced by FastQC (version 0.11.5)