##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727544.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4013535
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68433089533292	34.0	31.0	34.0	31.0	34.0
2	32.85346907402078	34.0	31.0	34.0	31.0	34.0
3	32.944292998566105	34.0	31.0	34.0	31.0	34.0
4	36.36870215408611	37.0	37.0	37.0	35.0	37.0
5	36.241613191363726	37.0	37.0	37.0	35.0	37.0
6	36.259015556112004	37.0	37.0	37.0	35.0	37.0
7	36.237234507734456	37.0	37.0	37.0	35.0	37.0
8	36.22612858739241	37.0	36.0	37.0	35.0	37.0
9	37.94971390557202	39.0	38.0	39.0	35.0	39.0
10	37.934093261924964	39.0	38.0	39.0	35.0	39.0
11	38.00070287165803	39.0	38.0	39.0	35.0	39.0
12	37.97858795301399	39.0	38.0	39.0	35.0	39.0
13	38.00273250388996	39.0	38.0	39.0	35.0	39.0
14	39.39774463160281	40.0	39.0	41.0	37.0	41.0
15	39.41427120979386	40.0	39.0	41.0	37.0	41.0
16	39.4001173529071	40.0	39.0	41.0	37.0	41.0
17	39.41448274401494	40.0	39.0	41.0	37.0	41.0
18	39.3875257098792	40.0	39.0	41.0	37.0	41.0
19	39.42166867860876	40.0	39.0	41.0	37.0	41.0
20	39.39916258360772	40.0	39.0	41.0	37.0	41.0
21	39.3793247100125	40.0	39.0	41.0	37.0	41.0
22	39.35350657213653	40.0	39.0	41.0	36.0	41.0
23	39.31686979184185	40.0	39.0	41.0	36.0	41.0
24	39.28021681634768	40.0	39.0	41.0	36.0	41.0
25	39.22164774942787	40.0	39.0	41.0	36.0	41.0
26	39.12899899963499	40.0	39.0	41.0	36.0	41.0
27	39.08825212686572	40.0	39.0	41.0	36.0	41.0
28	39.02819933051537	40.0	39.0	41.0	36.0	41.0
29	38.95474737357467	40.0	38.0	41.0	35.0	41.0
30	38.90347411944832	40.0	38.0	41.0	35.0	41.0
31	38.83927186383076	40.0	38.0	41.0	35.0	41.0
32	38.76024103439985	40.0	38.0	41.0	35.0	41.0
33	38.71349919709184	40.0	38.0	41.0	35.0	41.0
34	38.67056622154784	40.0	38.0	41.0	35.0	41.0
35	38.657752330551496	40.0	38.0	41.0	35.0	41.0
36	38.601854474920486	40.0	38.0	41.0	35.0	41.0
37	38.581286322406555	40.0	38.0	41.0	35.0	41.0
38	38.53822229032511	40.0	38.0	41.0	35.0	41.0
39	38.486148993343775	40.0	38.0	41.0	35.0	41.0
40	38.431204162913744	40.0	38.0	41.0	34.0	41.0
41	38.38073319405462	40.0	38.0	41.0	34.0	41.0
42	38.33859453075655	40.0	38.0	41.0	34.0	41.0
43	37.279549324971626	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	2.0
12	3.0
13	2.0
14	1.0
15	3.0
16	9.0
17	12.0
18	39.0
19	70.0
20	179.0
21	317.0
22	703.0
23	1313.0
24	2286.0
25	3716.0
26	5836.0
27	8905.0
28	13150.0
29	19260.0
30	27298.0
31	37959.0
32	51899.0
33	71097.0
34	103294.0
35	152511.0
36	230929.0
37	403025.0
38	972098.0
39	1907613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.230908662812205	16.476472735381652	11.591626832704835	27.7009917691013
2	18.832924093099972	19.4237000549391	35.00861460034608	26.73476125161485
3	20.326146402111853	21.326038018853705	29.082417370223506	29.265398208810932
4	15.502618016287387	14.104274660617136	33.04994225788489	37.34316506521059
5	16.403569421968413	35.201910535226425	32.872567449891434	15.52195259291373
6	37.59456439273608	32.30573546761147	14.683091090522446	15.416609049130006
7	31.16013688680926	29.493376786299358	20.166013252656324	19.180473074235056
8	28.212286674963593	34.078636414034015	19.0435115179013	18.665565393101094
9	28.95390223331801	13.766069063805347	17.44738740287552	39.83264130000112
10	17.12958775742581	25.745159815474388	32.5360561201036	24.589196306996204
11	37.95487020793391	20.29721928424693	20.387638328804904	21.36027217901426
12	22.635905753905224	23.04868401546268	29.14176654744508	25.173643683187013
13	32.092183075518214	18.66818154071162	23.129784591388887	26.109850792381277
14	23.727561862547606	18.892522427236838	23.783721831253494	33.59619387896206
15	26.976667700667868	26.110697925893263	21.04431629473768	25.868318078701197
16	25.951710898248052	24.76888827430183	23.294078661329724	25.98532216612039
17	25.716332360375578	24.32224958795675	23.79062347780697	26.1707945738607
18	25.522837099963002	24.65980737678879	24.45607176715788	25.36128375609033
19	26.959077222448542	24.011251926294403	24.2218144354042	24.807856415852857
20	25.860668961401856	23.553899492591942	24.319110210824125	26.26632133518208
21	25.546432259840763	24.24187156708488	25.128371871679207	25.083324301395155
22	26.369148394121396	23.34567905848585	23.774752182303132	26.510420365089626
23	25.58562464261555	23.904837007774944	24.597916799031278	25.91162155057823
24	25.303803255733413	23.711491241511535	24.50129374728263	26.483411755472417
25	26.11642853494488	23.28411238471821	24.05557694152412	26.54388213881279
26	25.645397386593118	23.779187175395254	24.510587300222873	26.06482813778876
27	26.446387037860642	24.23935508224047	23.756538811795586	25.557719068103307
28	25.73362385029656	24.725734296573968	23.134817561077703	26.405824292051772
29	25.716182866226404	24.716141755335382	24.260433757273823	25.307241621164383
30	26.944700868436428	23.73157328888374	23.570966741289162	25.75275910139067
31	26.1364358352425	23.8151405182713	24.34654238719732	25.701881259288882
32	25.598854874817338	24.12905331584252	24.184939211941593	26.087152597398557
33	25.523509823634278	23.5427870941701	24.31776476348157	26.61593831871405
34	26.112043373235817	23.826576820683012	24.916414083843794	25.144965722237377
35	26.113513399036016	23.68831964838976	24.21232155693173	25.985845395642492
36	25.58422936388994	23.545976302685787	24.823902121197396	26.045892212226878
37	25.13430180626306	23.47429385815746	25.3137446166534	26.077659718926082
38	25.838842815622638	22.890668699787096	24.87014564467483	26.400342839915435
39	24.656418842740877	23.476087787947534	25.956270469797822	25.91122289951377
40	25.97164345147108	22.526700277934538	25.802391158916016	25.69926511167836
41	24.823378891675294	23.122907860526944	25.95245836899392	26.101254878803847
42	24.649890931560332	23.283913059185977	26.19483821618598	25.871357793067705
43	24.185312947314525	22.8292266044771	25.844623256057314	27.140837192151057
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	185.0
2	238.0
3	586.0
4	934.0
5	934.0
6	1103.5
7	1273.0
8	1134.0
9	995.0
10	1366.5
11	1738.0
12	1738.0
13	2796.0
14	3854.0
15	4690.5
16	5527.0
17	5599.5
18	5672.0
19	5672.0
20	7229.0
21	8786.0
22	9630.0
23	10474.0
24	13643.0
25	16812.0
26	16812.0
27	21059.5
28	25307.0
29	30580.0
30	35853.0
31	44394.0
32	52935.0
33	52935.0
34	63975.5
35	75016.0
36	89611.5
37	104207.0
38	123229.0
39	142251.0
40	142251.0
41	163021.5
42	183792.0
43	208405.0
44	233018.0
45	266715.5
46	300413.0
47	300413.0
48	334361.0
49	368309.0
50	391790.0
51	415271.0
52	416448.5
53	417626.0
54	417626.0
55	396331.0
56	375036.0
57	349976.0
58	324916.0
59	303784.5
60	282653.0
61	282653.0
62	249214.5
63	215776.0
64	181607.0
65	147438.0
66	123428.0
67	99418.0
68	99418.0
69	81144.0
70	62870.0
71	51196.5
72	39523.0
73	31728.0
74	23933.0
75	23933.0
76	19204.0
77	14475.0
78	11489.0
79	8503.0
80	6612.0
81	4721.0
82	4721.0
83	3465.5
84	2210.0
85	1626.0
86	1042.0
87	725.0
88	408.0
89	408.0
90	261.0
91	114.0
92	78.0
93	42.0
94	30.0
95	18.0
96	18.0
97	10.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4013535.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.667128397848906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6461469756736	40.63420573788225
2	10.379428680853964	10.725505486999944
3	3.7693151307330655	5.842490664946197
4	1.874303884216292	3.8735959776956026
5	1.1496765126607764	2.9700241997817742
6	0.7736069142042631	2.39820286593932
7	0.5304728557443416	1.9185606404521536
8	0.416898141207569	1.723194383247681
9	0.3234958271235515	1.5042690392544782
>10	1.9648387754921535	18.86152487822327
>50	0.12478170429694249	4.348846786090869
>100	0.044477616686460594	3.9552849549559768
>500	0.0017368173971966444	0.6012336524466797
>1k	8.201637098386452E-4	0.6430607320838895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.491569152878946E-5
2	0.0	0.0	0.0	0.0	2.491569152878946E-5
3	0.0	0.0	0.0	0.0	2.491569152878946E-5
4	0.0	0.0	0.0	0.0	2.491569152878946E-5
5	0.0	0.0	0.0	0.0	2.491569152878946E-5
6	0.0	0.0	0.0	0.0	2.491569152878946E-5
7	0.0	0.0	0.0	0.0	2.491569152878946E-5
8	0.0	0.0	0.0	0.0	4.983138305757892E-5
9	0.0	0.0	0.0	0.0	7.474707458636838E-5
10	0.0	0.0	0.0	9.966276611515784E-5	7.474707458636838E-5
11	0.0	0.0	0.0	1.245784576439473E-4	7.474707458636838E-5
12	0.0	0.0	0.0	1.245784576439473E-4	7.474707458636838E-5
13	0.0	0.0	0.0	1.245784576439473E-4	7.474707458636838E-5
14	0.0	0.0	0.0	1.4949414917273676E-4	1.4949414917273676E-4
15	0.0	0.0	0.0	2.2424122375910513E-4	2.2424122375910513E-4
16	0.0	0.0	0.0	2.491569152878946E-4	3.7373537293184186E-4
17	0.0	0.0	0.0	4.733981390469997E-4	3.9865106446063136E-4
18	0.0	0.0	0.0	6.976393628061048E-4	4.235667559894208E-4
19	0.0	0.0	0.0	8.969648950364205E-4	4.235667559894208E-4
20	0.0	0.0	0.0	0.001245784576439473	4.235667559894208E-4
21	0.0	0.0	0.0	0.0019185082477167882	4.235667559894208E-4
22	0.0	0.0	0.0	0.002665978993580472	4.235667559894208E-4
23	0.0	0.0	0.0	0.0035629438886168926	4.983138305757891E-4
24	0.0	0.0	0.0	0.0051824638379882075	5.232295221045786E-4
25	0.0	0.0	0.0	0.00645316410595647	5.232295221045786E-4
26	0.0	0.0	0.0	0.008521166502845994	5.48145213633368E-4
27	0.0	0.0	0.0	0.017715056676969303	5.730609051621576E-4
28	0.0	0.0	0.0	0.046243523477433233	5.730609051621576E-4
29	0.0	0.0	0.0	0.08969648950364205	5.730609051621576E-4
30	0.0	0.0	0.0	0.14545780714507286	5.730609051621576E-4
31	0.0	0.0	0.0	0.3961345796162236	5.730609051621576E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1980	0.0	19.34091	37
TACTTAC	1125	0.0	14.8	31
TCTTATA	3300	0.0	14.183333	37
GTACTTA	1245	0.0	13.967871	30
TTACATA	690	0.0	13.673912	34
ACTTACA	1050	0.0	13.390475	32
TCGTTAC	1320	0.0	13.314394	23
TACGGAT	2490	0.0	13.299196	27
CGTTACG	1115	0.0	13.273542	24
GTATAGG	420	0.0	13.214285	1
ACGGATG	2615	0.0	13.017209	28
CGTATAC	160	2.6995604E-6	12.71875	3
GTACCGT	365	0.0	12.671233	6
GTCTTAG	595	0.0	12.436975	1
CTCGTTA	1535	0.0	12.41368	22
GTTACGG	1925	0.0	12.397403	25
GGTATCA	2920	0.0	12.354452	1
AGTGTAC	1455	0.0	12.333333	27
TACATAT	695	0.0	12.244604	35
TACCGTC	320	0.0	12.140625	7
>>END_MODULE