FastQCFastQC Report
Thu 9 Feb 2017
SRR2727543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034448
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7100.020.84507237
CTATAGT656.9012756E-519.9230774
TAGTACA851.2450619E-619.5882364
TACTTAC3150.018.79365231
TATAGTG902.1517917E-618.55
TATACCG500.007034418618.55
ACTTACA2750.018.16363732
GTACTTA3350.017.67164230
CTTATTC752.0670143E-417.2666663
ATCTATA803.3821558E-416.18752
CAATACT803.3821558E-416.18754
GTACTAG700.002592258215.8571431
GGACCGT700.002592258215.8571436
CGTTACA1059.341213E-615.85714224
TTACACT1302.5880217E-715.65384634
ATCCTAT855.3635065E-415.2352941
TATTAAG855.3635065E-415.2352942
CATATAA1101.4515101E-515.13636437
ATATGAC1001.0931958E-414.83
TACATAT1508.0970494E-814.835
Produced by FastQC (version 0.11.5)