##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1034448
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31838043091581	33.0	31.0	34.0	31.0	34.0
2	32.497269074907585	34.0	31.0	34.0	31.0	34.0
3	32.589343301934946	34.0	31.0	34.0	31.0	34.0
4	36.123823527137176	37.0	35.0	37.0	35.0	37.0
5	35.93368347176465	37.0	35.0	37.0	35.0	37.0
6	35.945059587335464	37.0	35.0	37.0	35.0	37.0
7	35.9195416299321	37.0	35.0	37.0	35.0	37.0
8	35.90311934480999	37.0	35.0	37.0	35.0	37.0
9	37.592005591387874	39.0	37.0	39.0	35.0	39.0
10	37.515908001175504	39.0	37.0	39.0	35.0	39.0
11	37.613762122407316	39.0	37.0	39.0	35.0	39.0
12	37.573884815863146	39.0	37.0	39.0	35.0	39.0
13	37.61574868915596	39.0	37.0	39.0	35.0	39.0
14	38.90172246454148	40.0	38.0	41.0	36.0	41.0
15	38.903942972483875	40.0	38.0	41.0	36.0	41.0
16	38.88734281471857	40.0	38.0	41.0	36.0	41.0
17	38.90067746276275	40.0	38.0	41.0	36.0	41.0
18	38.858706285864535	40.0	38.0	41.0	36.0	41.0
19	38.90238755355513	40.0	38.0	41.0	36.0	41.0
20	38.8762509086972	40.0	38.0	41.0	35.0	41.0
21	38.8493679720972	40.0	38.0	41.0	35.0	41.0
22	38.821293095448006	40.0	38.0	41.0	35.0	41.0
23	38.777782933506565	40.0	38.0	41.0	35.0	41.0
24	38.73103432942013	40.0	38.0	41.0	35.0	41.0
25	38.66031255316845	40.0	38.0	41.0	35.0	41.0
26	38.543484061064454	40.0	38.0	41.0	34.0	41.0
27	38.49551354925526	40.0	38.0	41.0	34.0	41.0
28	38.41207967921056	40.0	38.0	41.0	34.0	41.0
29	38.32267064173356	40.0	38.0	41.0	34.0	41.0
30	38.264933568434564	40.0	38.0	41.0	34.0	41.0
31	38.18871224073129	40.0	38.0	41.0	34.0	41.0
32	38.09495499048768	40.0	38.0	41.0	34.0	41.0
33	38.0517860733454	40.0	38.0	41.0	33.0	41.0
34	38.00322877515351	40.0	38.0	41.0	33.0	41.0
35	37.993000131471085	40.0	38.0	41.0	33.0	41.0
36	37.92683634170108	40.0	37.0	41.0	33.0	41.0
37	37.909390322181494	40.0	37.0	41.0	33.0	41.0
38	37.875669922509395	40.0	37.0	41.0	33.0	41.0
39	37.81981501245109	40.0	37.0	41.0	33.0	41.0
40	37.77982363540748	40.0	37.0	41.0	33.0	41.0
41	37.7234612082966	40.0	37.0	41.0	33.0	41.0
42	37.695410499126105	40.0	37.0	41.0	33.0	41.0
43	36.559987548914975	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	6.0
18	14.0
19	41.0
20	79.0
21	144.0
22	320.0
23	561.0
24	977.0
25	1603.0
26	2443.0
27	3715.0
28	5317.0
29	7454.0
30	10402.0
31	14202.0
32	19434.0
33	26084.0
34	36968.0
35	52409.0
36	80456.0
37	137452.0
38	306877.0
39	327487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60431843843286	16.496914296320355	11.707790048412294	28.190977216834483
2	19.091534808902914	19.462940621471546	34.240580483504246	27.20494408612129
3	20.525729664516728	21.159304285957344	28.88787063245325	29.427095417072678
4	16.005057769941068	14.271282848437043	32.332219695915114	37.39143968570677
5	16.741972530277017	34.76743151903237	32.71193912115447	15.77865682953614
6	38.27210260931434	31.925626034368086	14.257362380709326	15.544908975608246
7	31.35034337153728	29.307128052835907	19.755947133157008	19.5865814424698
8	28.858386308446633	33.87188142854933	18.588657912239185	18.681074350764852
9	29.298911110079967	13.310963915054213	16.736075665475695	40.654049309390125
10	17.136772462221398	25.795883408349184	32.12128594187431	24.9460581875551
11	38.96638593723416	19.987278239215502	19.52848282368954	21.517852999860796
12	23.050264488886825	22.726226934558337	28.6080112291773	25.615497347377534
13	32.89348522110343	18.158476791486862	22.430223655514826	26.517814331894883
14	23.947361298006278	18.38951788780103	23.365408411055945	34.29771240313674
15	27.763696193525433	25.46459561041251	20.54081017122175	26.2308980248403
16	26.39929701637975	24.339744482081265	22.847160997942865	26.413797503596122
17	26.26434581535273	23.685289159049077	23.22204692744343	26.828318098154764
18	26.076902850602444	24.473535644130976	23.689059285725335	25.76050221954124
19	27.759732727019625	23.74599786552828	23.43646079841616	25.05780860903593
20	26.548555364793593	23.078588773916138	23.448544537763137	26.92431132352714
21	26.155688831144726	23.972398805933214	24.355791687934047	25.516120674988013
22	27.074053021514853	22.865818291494595	22.83440056919246	27.22572811779809
23	26.210984022396488	23.53728751952732	23.804869843626747	26.446858614449447
24	25.78515304780907	23.073368598518243	23.86026170479328	27.281216648879404
25	26.785686665738634	22.702252795693937	23.220790218551343	27.291270320016086
26	26.144958470604614	23.217310101619415	23.904439855845823	26.733291571930152
27	27.24786552828175	23.59857624549518	23.111649884769463	26.041908341453606
28	26.286289886006838	24.467445439500104	22.338387236477814	26.907877438015248
29	26.42017771797132	24.232440876680123	23.48846921256554	25.858912192783013
30	27.677853309204526	23.150317850679784	22.73212379935966	26.439705040756035
31	26.57987641718095	23.431047283188715	23.743484447744112	26.245591851886225
32	26.1374182172521	23.48479575580406	23.521143643759764	26.856642383184077
33	26.095173464495076	23.01817007718126	23.56387174609067	27.322784712233002
34	26.692110188235656	23.405236415943577	24.36130187307627	25.5413515227445
35	26.574559571868285	23.026097010192874	23.705106491578118	26.694236926360727
36	26.075936151454687	22.96500162405457	24.337037724467542	26.6220245000232
37	25.488473079362134	22.760351414474194	25.033641130341977	26.717534375821693
38	26.29325011987069	22.064811377662288	24.413600296969978	27.22833820549704
39	24.79496311076052	22.77398187245758	25.900673595966158	26.53038142081574
40	26.457395635160008	21.538347021793268	25.70926716471007	26.294990178336658
41	25.10914033378188	22.155004408148113	25.987096499775724	26.74875875829428
42	24.626467449306297	22.093619012265478	26.528061342861122	26.751852195567103
43	24.12929407761434	21.64391056872844	26.149695296428625	28.077100057228588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	9.0
2	14.0
3	25.0
4	36.0
5	36.0
6	49.5
7	63.0
8	63.0
9	63.0
10	94.5
11	126.0
12	126.0
13	185.5
14	245.0
15	361.5
16	478.0
17	482.0
18	486.0
19	486.0
20	674.5
21	863.0
22	1110.0
23	1357.0
24	1819.0
25	2281.0
26	2281.0
27	3106.5
28	3932.0
29	5037.0
30	6142.0
31	8057.0
32	9972.0
33	9972.0
34	12614.5
35	15257.0
36	18938.5
37	22620.0
38	27168.5
39	31717.0
40	31717.0
41	37305.5
42	42894.0
43	49548.5
44	56203.0
45	65535.0
46	74867.0
47	74867.0
48	84842.0
49	94817.0
50	103949.0
51	113081.0
52	114142.0
53	115203.0
54	115203.0
55	109113.5
56	103024.0
57	96285.0
58	89546.0
59	84134.0
60	78722.0
61	78722.0
62	69312.0
63	59902.0
64	50405.0
65	40908.0
66	33931.5
67	26955.0
68	26955.0
69	22144.5
70	17334.0
71	13942.0
72	10550.0
73	8450.5
74	6351.0
75	6351.0
76	5151.0
77	3951.0
78	3152.5
79	2354.0
80	1815.5
81	1277.0
82	1277.0
83	890.5
84	504.0
85	361.0
86	218.0
87	154.5
88	91.0
89	91.0
90	58.0
91	25.0
92	17.0
93	9.0
94	6.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1034448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.54841852138672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.98641966757766	59.10671083674903
2	8.311019223252906	11.560364865561668
3	2.711840309649018	5.658126144559106
4	1.2561440946979465	3.494513408848849
5	0.7426359138849019	2.5824576673939834
6	0.46118126156085065	1.9244656435953098
7	0.3291812894009285	1.602582665926585
8	0.23289070764835682	1.2957744324215874
9	0.17303434325918113	1.0830838431206538
>10	0.7531883674135673	8.44370607549601
>50	0.02827130853006972	1.3532039203099062
>100	0.01391520929053237	1.7636828075177735
>500	2.7830383403186477E-4	0.1313276884995315
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.666991477580313E-5
2	0.0	0.0	0.0	0.0	9.666991477580313E-5
3	0.0	0.0	0.0	0.0	9.666991477580313E-5
4	0.0	9.666991477580313E-5	0.0	0.0	9.666991477580313E-5
5	0.0	9.666991477580313E-5	0.0	0.0	9.666991477580313E-5
6	0.0	9.666991477580313E-5	0.0	0.0	1.9333982955160625E-4
7	0.0	9.666991477580313E-5	0.0	0.0	1.9333982955160625E-4
8	0.0	9.666991477580313E-5	0.0	0.0	1.9333982955160625E-4
9	0.0	9.666991477580313E-5	0.0	9.666991477580313E-5	1.9333982955160625E-4
10	0.0	9.666991477580313E-5	0.0	9.666991477580313E-5	1.9333982955160625E-4
11	0.0	9.666991477580313E-5	0.0	9.666991477580313E-5	1.9333982955160625E-4
12	0.0	9.666991477580313E-5	0.0	9.666991477580313E-5	1.9333982955160625E-4
13	0.0	1.9333982955160625E-4	0.0	1.9333982955160625E-4	1.9333982955160625E-4
14	0.0	1.9333982955160625E-4	0.0	1.9333982955160625E-4	3.866796591032125E-4
15	0.0	1.9333982955160625E-4	0.0	2.900097443274094E-4	3.866796591032125E-4
16	0.0	1.9333982955160625E-4	0.0	3.866796591032125E-4	4.8334957387901565E-4
17	0.0	1.9333982955160625E-4	0.0	5.800194886548188E-4	4.8334957387901565E-4
18	0.0	1.9333982955160625E-4	0.0	7.73359318206425E-4	4.8334957387901565E-4
19	9.666991477580313E-5	1.9333982955160625E-4	0.0	7.73359318206425E-4	4.8334957387901565E-4
20	9.666991477580313E-5	1.9333982955160625E-4	0.0	9.666991477580313E-4	4.8334957387901565E-4
21	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.0011600389773096377	4.8334957387901565E-4
22	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.00154671863641285	4.8334957387901565E-4
23	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.002030068210291866	8.700292329822282E-4
24	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.002900097443274094	8.700292329822282E-4
25	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.0031901071876015035	8.700292329822282E-4
26	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.005413515227444976	8.700292329822282E-4
27	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.013533788068612438	8.700292329822282E-4
28	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.04408148113776623	8.700292329822282E-4
29	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.0927064482699952	8.700292329822282E-4
30	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.15389850432307858	8.700292329822282E-4
31	9.666991477580313E-5	1.9333982955160625E-4	0.0	0.4382047236787156	8.700292329822282E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	710	0.0	20.845072	37
CTATAGT	65	6.9012756E-5	19.923077	4
TAGTACA	85	1.2450619E-6	19.588236	4
TACTTAC	315	0.0	18.793652	31
TATAGTG	90	2.1517917E-6	18.5	5
TATACCG	50	0.0070344186	18.5	5
ACTTACA	275	0.0	18.163637	32
GTACTTA	335	0.0	17.671642	30
CTTATTC	75	2.0670143E-4	17.266666	3
ATCTATA	80	3.3821558E-4	16.1875	2
CAATACT	80	3.3821558E-4	16.1875	4
GTACTAG	70	0.0025922582	15.857143	1
GGACCGT	70	0.0025922582	15.857143	6
CGTTACA	105	9.341213E-6	15.857142	24
TTACACT	130	2.5880217E-7	15.653846	34
ATCCTAT	85	5.3635065E-4	15.235294	1
TATTAAG	85	5.3635065E-4	15.235294	2
CATATAA	110	1.4515101E-5	15.136364	37
ATATGAC	100	1.0931958E-4	14.8	3
TACATAT	150	8.0970494E-8	14.8	35
>>END_MODULE