FastQCFastQC Report
Thu 9 Feb 2017
SRR2727542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926081
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11900.021.14285737
TACTTAC5850.018.3418831
GTACTTA6150.017.74796730
TATACCG650.001580458117.0769235
GTATAGA1901.8189894E-1216.5526311
TCTTATA20100.016.38308537
CGATAGC855.36649E-415.2352934
AGTGTAC7850.015.08280327
CTATGCG750.004105744414.89
CTATACC1551.2113378E-714.322584
CTACAGT10050.014.1741323
TAAAACG800.00630065813.8750014
TAAAGTG2601.8189894E-1213.5192315
GTGTACT8650.013.26011728
TATACAG3350.013.2537325
CAGTGTA9250.013.226
TCGTTAC7300.013.17808223
ACTAGAC2254.0745363E-1013.1555563
CGAACGT850.009407748513.05882310
CTTAGCG850.009407748513.0588233
Produced by FastQC (version 0.11.5)