##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1926081
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.282556133412875	33.0	31.0	34.0	30.0	34.0
2	32.46362691911711	34.0	31.0	34.0	31.0	34.0
3	32.55819718900711	34.0	31.0	34.0	31.0	34.0
4	36.10401743228867	37.0	35.0	37.0	35.0	37.0
5	35.902874801215525	37.0	35.0	37.0	35.0	37.0
6	35.91526213072036	37.0	35.0	37.0	35.0	37.0
7	35.89025487505458	37.0	35.0	37.0	35.0	37.0
8	35.87491959060912	37.0	35.0	37.0	35.0	37.0
9	37.547617675476786	39.0	37.0	39.0	35.0	39.0
10	37.47622400096361	39.0	37.0	39.0	35.0	39.0
11	37.57001496821785	39.0	37.0	39.0	35.0	39.0
12	37.53282390512133	39.0	37.0	39.0	35.0	39.0
13	37.57582053921927	39.0	37.0	39.0	35.0	39.0
14	38.850250846148214	40.0	38.0	41.0	36.0	41.0
15	38.85219728557625	40.0	38.0	41.0	36.0	41.0
16	38.831782256301786	40.0	38.0	41.0	36.0	41.0
17	38.84765490132554	40.0	38.0	41.0	36.0	41.0
18	38.81411996691728	40.0	38.0	41.0	35.0	41.0
19	38.86153645667031	40.0	38.0	41.0	35.0	41.0
20	38.83404488180923	40.0	38.0	41.0	35.0	41.0
21	38.8123687425399	40.0	38.0	41.0	35.0	41.0
22	38.78612737470542	40.0	38.0	41.0	35.0	41.0
23	38.74191012735186	40.0	38.0	41.0	35.0	41.0
24	38.693011353105085	40.0	38.0	41.0	35.0	41.0
25	38.63333940784422	40.0	38.0	41.0	35.0	41.0
26	38.516203627988645	40.0	38.0	41.0	34.0	41.0
27	38.469649511105715	40.0	38.0	41.0	34.0	41.0
28	38.38935070747284	40.0	38.0	41.0	34.0	41.0
29	38.298757944240144	40.0	38.0	41.0	34.0	41.0
30	38.24205627904538	40.0	38.0	41.0	34.0	41.0
31	38.16474021601376	40.0	38.0	41.0	34.0	41.0
32	38.06930341974195	40.0	38.0	41.0	34.0	41.0
33	38.02579694208084	40.0	38.0	41.0	33.0	41.0
34	37.97114503491805	40.0	38.0	41.0	33.0	41.0
35	37.95998195299159	40.0	38.0	41.0	33.0	41.0
36	37.89534708041874	40.0	37.0	41.0	33.0	41.0
37	37.8806057481487	40.0	37.0	41.0	33.0	41.0
38	37.8438082302873	40.0	37.0	41.0	33.0	41.0
39	37.78468247181713	40.0	37.0	41.0	33.0	41.0
40	37.73103571448968	40.0	37.0	41.0	33.0	41.0
41	37.68206581135477	40.0	37.0	41.0	33.0	41.0
42	37.63642339029356	40.0	37.0	41.0	33.0	41.0
43	36.54839697811255	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	3.0
13	3.0
14	2.0
15	6.0
16	11.0
17	9.0
18	36.0
19	67.0
20	174.0
21	305.0
22	559.0
23	1051.0
24	1905.0
25	2980.0
26	4706.0
27	7098.0
28	10281.0
29	14537.0
30	20224.0
31	27660.0
32	36771.0
33	49568.0
34	69636.0
35	98517.0
36	150007.0
37	260292.0
38	576727.0
39	592942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27850178678882	16.8888535840393	12.174721623856941	27.657923005314938
2	18.227841923574346	19.769936986035376	35.680067453030276	26.322153637360007
3	19.93239121303829	21.67343948670902	29.507741367055694	28.886427933196995
4	15.40667292808558	14.547415191780615	33.44324563712534	36.60266624300847
5	16.093559928165018	35.32219049977649	33.22456324526331	15.359686326795185
6	36.949121039042495	32.985736321577335	14.770406852048279	15.294735787331891
7	30.693101691984914	30.054239671124943	20.263893366893708	18.98876526999643
8	28.030337249575695	34.85762021431082	18.984611758280156	18.127430777833332
9	28.9149833262464	14.060364024150592	17.512970638306488	39.511682011296514
10	16.887711368317323	26.355174055504417	32.410423029976414	24.34669154620185
11	37.81009209893042	20.77711165833628	20.31726599244788	21.095530250285425
12	22.539862030724564	23.176751133519307	29.473630652085763	24.809756183670366
13	31.92259307889959	18.79116195009452	23.36324380958018	25.923001161425713
14	23.38328450361122	18.885290909364663	24.47622919285326	33.255195394170855
15	26.886927392980876	26.072683339901076	21.36909091569877	25.67129835141928
16	25.54658916213804	25.063068479466853	23.706791147412805	25.6835512109823
17	25.367572807166468	24.64922295583623	24.12816491102918	25.85503932596812
18	25.256310612066677	25.115973834952943	24.74766118351201	24.880054369468365
19	26.896013199860235	24.388538176743346	24.407696249534677	24.307752373861742
20	25.540981921321066	23.93237875250314	24.494971914472963	26.03166741170283
21	25.274534144721848	24.753995288879334	25.288759922350096	24.68271064404872
22	26.259227934858398	23.592154224043536	23.851281436242814	26.297336404855248
23	25.356098731050253	24.05994348108932	24.85404300234518	25.729914785515252
24	24.934932643019685	23.708712146581583	24.91525538126382	26.441099829134913
25	25.809869886053598	23.46313576635666	24.32057634128575	26.406418006303994
26	25.284554491737367	23.893699174645302	25.02745211649977	25.794294217117557
27	26.232437784288408	24.406605952709153	24.12494593944907	25.23601032355337
28	25.494722184581022	25.05984950788674	23.452596230376603	25.99283207715563
29	25.448618204530337	24.94619904355009	24.594604276767175	25.010578475152396
30	26.78651624723986	24.081385985324605	23.679741402360545	25.45235636507499
31	25.709095308037412	24.10568402886483	24.663396814568028	25.521823848529735
32	25.301531970877654	24.41029219435735	24.42316808067781	25.865007754087184
33	25.327439500207937	23.68623126441723	24.486041864282967	26.50028737109187
34	25.706499363214736	24.01145123180178	25.376814370735186	24.9052350342483
35	25.649232820426555	23.779737196929933	24.692211802099703	25.87881818054381
36	25.20854522732948	23.606795352843417	25.381590909208906	25.803068510618193
37	24.61127024252874	23.520973417005827	25.96853403361541	25.89922230685002
38	25.61610856448924	22.811605534762037	25.38434261072094	26.187943290027782
39	24.17837048389969	23.432867049724283	26.692958395830708	25.695804070545318
40	25.60193470575744	22.485606783930688	26.481233136093447	25.431225374218425
41	24.42643897115438	23.086775685965442	26.587978387201783	25.898806955678396
42	24.074480772096294	23.12057488755665	27.022851063896063	25.78209327645099
43	23.59028514377121	22.69826658380411	26.583461443210332	27.127986829214347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	77.0
2	107.0
3	211.0
4	315.0
5	315.0
6	426.0
7	537.0
8	507.5
9	478.0
10	649.0
11	820.0
12	820.0
13	1440.0
14	2060.0
15	3003.5
16	3947.0
17	3784.0
18	3621.0
19	3621.0
20	4346.0
21	5071.0
22	5387.5
23	5704.0
24	7388.0
25	9072.0
26	9072.0
27	11489.5
28	13907.0
29	16999.0
30	20091.0
31	24538.5
32	28986.0
33	28986.0
34	34919.0
35	40852.0
36	47798.0
37	54744.0
38	63898.0
39	73052.0
40	73052.0
41	83252.0
42	93452.0
43	104601.5
44	115751.0
45	131691.0
46	147631.0
47	147631.0
48	163053.0
49	178475.0
50	189102.5
51	199730.0
52	200166.0
53	200602.0
54	200602.0
55	188581.0
56	176560.0
57	162487.0
58	148414.0
59	138548.5
60	128683.0
61	128683.0
62	112476.5
63	96270.0
64	80799.0
65	65328.0
66	54212.0
67	43096.0
68	43096.0
69	35307.5
70	27519.0
71	22325.5
72	17132.0
73	13758.5
74	10385.0
75	10385.0
76	8361.0
77	6337.0
78	5006.5
79	3676.0
80	2859.5
81	2043.0
82	2043.0
83	1468.0
84	893.0
85	674.0
86	455.0
87	312.5
88	170.0
89	170.0
90	103.0
91	36.0
92	29.0
93	22.0
94	16.0
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1926081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.21743535472828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66647784771767	52.259627193411696
2	9.146490358845648	11.564353259659404
3	3.158011603997437	5.989241832755692
4	1.4942412212759455	3.7784839124152922
5	0.8448920210319579	2.6705953360656762
6	0.5814973225490144	2.2056461638313936
7	0.3997457561696906	1.7689631049289074
8	0.2936439328541607	1.485073307401286
9	0.21875440072015936	1.24461829674801
>10	1.1353918854000802	12.111288067768058
>50	0.038935434359535656	1.640383049521849
>100	0.020270914592456248	2.3725486886187483
>500	0.001153110340455949	0.49278154601538365
>1k	4.941901459096892E-4	0.41639624085851695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.19188964534721E-5	0.0
4	0.0	0.0	0.0	5.19188964534721E-5	0.0
5	0.0	0.0	0.0	5.19188964534721E-5	0.0
6	0.0	0.0	0.0	5.19188964534721E-5	5.19188964534721E-5
7	0.0	0.0	0.0	1.038377929069442E-4	5.19188964534721E-5
8	0.0	0.0	0.0	1.038377929069442E-4	5.19188964534721E-5
9	0.0	0.0	0.0	1.557566893604163E-4	1.038377929069442E-4
10	0.0	0.0	0.0	1.557566893604163E-4	1.038377929069442E-4
11	0.0	0.0	0.0	2.076755858138884E-4	1.038377929069442E-4
12	0.0	0.0	0.0	2.076755858138884E-4	1.038377929069442E-4
13	0.0	0.0	0.0	2.076755858138884E-4	1.038377929069442E-4
14	0.0	0.0	0.0	2.595944822673605E-4	1.557566893604163E-4
15	0.0	0.0	0.0	2.595944822673605E-4	2.076755858138884E-4
16	0.0	0.0	0.0	2.595944822673605E-4	2.076755858138884E-4
17	0.0	0.0	0.0	3.6343227517430474E-4	2.076755858138884E-4
18	0.0	0.0	0.0	5.19188964534721E-4	2.595944822673605E-4
19	0.0	0.0	0.0	6.230267574416652E-4	2.595944822673605E-4
20	0.0	5.19188964534721E-5	0.0	9.345401361624978E-4	2.595944822673605E-4
21	0.0	5.19188964534721E-5	0.0	0.0012460535148833304	2.595944822673605E-4
22	0.0	5.19188964534721E-5	0.0	0.001609485790057635	2.595944822673605E-4
23	0.0	5.19188964534721E-5	0.0	0.0019209991687784678	3.115133787208326E-4
24	0.0	5.19188964534721E-5	0.0	0.0028555393049409655	3.115133787208326E-4
25	0.0	5.19188964534721E-5	0.0	0.0040496739233708235	3.115133787208326E-4
26	0.0	5.19188964534721E-5	0.0	0.0062302675744166525	3.115133787208326E-4
27	0.0	5.19188964534721E-5	0.0	0.01682172245092496	3.6343227517430474E-4
28	0.0	5.19188964534721E-5	0.0	0.05337262555416932	3.6343227517430474E-4
29	0.0	5.19188964534721E-5	0.0	0.1073682778657803	3.6343227517430474E-4
30	0.0	5.19188964534721E-5	0.0	0.17688768021697945	3.6343227517430474E-4
31	0.0	5.19188964534721E-5	0.0	0.5240693408013474	3.6343227517430474E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1190	0.0	21.142857	37
TACTTAC	585	0.0	18.34188	31
GTACTTA	615	0.0	17.747967	30
TATACCG	65	0.0015804581	17.076923	5
GTATAGA	190	1.8189894E-12	16.552631	1
TCTTATA	2010	0.0	16.383085	37
CGATAGC	85	5.36649E-4	15.235293	4
AGTGTAC	785	0.0	15.082803	27
CTATGCG	75	0.0041057444	14.8	9
CTATACC	155	1.2113378E-7	14.32258	4
CTACAGT	1005	0.0	14.17413	23
TAAAACG	80	0.006300658	13.875001	4
TAAAGTG	260	1.8189894E-12	13.519231	5
GTGTACT	865	0.0	13.260117	28
TATACAG	335	0.0	13.253732	5
CAGTGTA	925	0.0	13.2	26
TCGTTAC	730	0.0	13.178082	23
ACTAGAC	225	4.0745363E-10	13.155556	3
CGAACGT	85	0.0094077485	13.058823	10
CTTAGCG	85	0.0094077485	13.058823	3
>>END_MODULE