FastQCFastQC Report
Thu 9 Feb 2017
SRR2727541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662972
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50140.1882858700729861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40120.15065873768105711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35840.13458646955356646No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT29000.10890088217224965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16450.020.130737
TCTTATA28850.015.83882237
CGTTACG6700.015.46268624
TCGTTAC7750.015.03870923
GTTACGG10550.014.55450225
TACTTAC10750.014.45581431
CTCGTTA8800.014.29545422
GTACTAG1956.7484507E-1014.230771
CGACTTA1601.7859747E-713.87519
CGGTATA1203.3035692E-513.87499914
GTCTTAG3950.013.582281
TACGGAT14300.013.32517427
ACGGATG14500.013.26896628
ACTTACA10100.013.00495132
GTACTTA12550.012.97211330
AAGCGTG2001.4648322E-812.9500017
GATCTCG10950.012.67123319
GATGGTT30950.012.61227931
AGTGTAC13950.012.59856627
CGGATGG16500.012.55757529
Produced by FastQC (version 0.11.5)