FastQCFastQC Report
Thu 9 Feb 2017
SRR2727540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650716
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.1521763889124477No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT23800.14417985892182544No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG22640.13715260529370285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18320.11098214350621184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17510.10607518192105728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10500.019.3809537
CGACTTA1801.0913936E-1116.44444519
TTAACCT1455.349466E-815.3103454
ATAACAC750.004105414714.83
TATAACA1508.107054E-814.84
TCTTATA17050.014.5395937
GTATATA908.27671E-414.3888881
TACTGTG3500.014.2714297
CTTGTTA7150.014.23076922
TACCTAT800.006300156513.8756
TAAGTCG2750.013.45454620
GTATTAT1102.4573784E-413.4545451
GTGTAAG2501.0913936E-1113.321
GGGCGTC3900.013.28205211
GTATAGA1951.0264557E-813.2820521
CTCTTAT27650.013.2477437
TGTACTA1551.8887367E-613.1290325
TGTACCG1551.8887367E-613.1290325
TATAGTG2001.463377E-812.9499995
GTCTATA1153.5786387E-412.8695651
Produced by FastQC (version 0.11.5)