##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727540.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650716
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61224341437291	34.0	31.0	34.0	31.0	34.0
2	32.77785518526506	34.0	31.0	34.0	31.0	34.0
3	32.87540679317339	34.0	31.0	34.0	31.0	34.0
4	36.32333423799127	37.0	37.0	37.0	35.0	37.0
5	36.186253116829306	37.0	35.0	37.0	35.0	37.0
6	36.214672299777796	37.0	36.0	37.0	35.0	37.0
7	36.18649422432448	37.0	36.0	37.0	35.0	37.0
8	36.18133161609871	37.0	36.0	37.0	35.0	37.0
9	37.896305603144334	39.0	38.0	39.0	35.0	39.0
10	37.87478766789684	39.0	38.0	39.0	35.0	39.0
11	37.9512005699345	39.0	38.0	39.0	35.0	39.0
12	37.92756961221676	39.0	38.0	39.0	35.0	39.0
13	37.953945439433554	39.0	38.0	39.0	35.0	39.0
14	39.311036544142056	40.0	39.0	41.0	36.0	41.0
15	39.33260476060085	40.0	39.0	41.0	36.0	41.0
16	39.31046345949273	40.0	39.0	41.0	36.0	41.0
17	39.3264480383058	40.0	39.0	41.0	36.0	41.0
18	39.307376314278166	40.0	39.0	41.0	36.0	41.0
19	39.35072962278187	40.0	39.0	41.0	36.0	41.0
20	39.32826724887867	40.0	39.0	41.0	36.0	41.0
21	39.30438549090213	40.0	39.0	41.0	36.0	41.0
22	39.27357885911326	40.0	39.0	41.0	36.0	41.0
23	39.23194419875981	40.0	39.0	41.0	36.0	41.0
24	39.19392675663167	40.0	39.0	41.0	36.0	41.0
25	39.13779293349068	40.0	39.0	41.0	36.0	41.0
26	39.04363803343519	40.0	39.0	41.0	36.0	41.0
27	38.997707661402686	40.0	39.0	41.0	35.0	41.0
28	38.92582309737108	40.0	38.0	41.0	35.0	41.0
29	38.84411734059645	40.0	38.0	41.0	35.0	41.0
30	38.777313602097514	40.0	38.0	41.0	35.0	41.0
31	38.713908994642324	40.0	38.0	41.0	35.0	41.0
32	38.62628398828145	40.0	38.0	41.0	35.0	41.0
33	38.58082553267794	40.0	38.0	41.0	35.0	41.0
34	38.53416032800312	40.0	38.0	41.0	35.0	41.0
35	38.51823572316498	40.0	38.0	41.0	35.0	41.0
36	38.45734941685911	40.0	38.0	41.0	34.0	41.0
37	38.437161813419145	40.0	38.0	41.0	34.0	41.0
38	38.3920559320925	40.0	38.0	41.0	34.0	41.0
39	38.343687830008314	40.0	38.0	41.0	34.0	41.0
40	38.280797544822974	40.0	38.0	41.0	34.0	41.0
41	38.225580293642274	40.0	38.0	41.0	34.0	41.0
42	38.17292193205857	40.0	38.0	41.0	34.0	41.0
43	37.11595453124583	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	1.0
14	2.0
15	3.0
16	3.0
17	8.0
18	9.0
19	41.0
20	96.0
21	167.0
22	353.0
23	592.0
24	972.0
25	1640.0
26	2671.0
27	4104.0
28	5920.0
29	8594.0
30	12225.0
31	16709.0
32	22822.0
33	31328.0
34	45643.0
35	66168.0
36	101645.0
37	176702.0
38	417647.0
39	734647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99933119930988	16.585711897140392	12.002912675469311	27.412044228080422
2	17.913196455356342	19.8709529682877	35.607275873015105	26.608574703340853
3	20.169005449756348	21.675563815944113	29.48011650701877	28.67531422728077
4	15.272403005726002	14.333295370009136	33.313362201614325	37.08093942265053
5	16.28887101112487	35.22192793914883	32.994227959261316	15.494973090464986
6	37.098628716266155	32.62329801128722	14.650067001228559	15.628006271218064
7	30.496099874236393	30.043811291584987	20.294769057790678	19.165319776387943
8	28.155479198117668	34.43111958689442	19.20863431383715	18.204766901150773
9	28.599347192369855	14.236791792167763	17.54353868260803	39.62032233285435
10	17.010921321414465	26.242794036042543	32.3488716411545	24.397413001388486
11	37.35815246232544	20.809273066960035	20.114120175729806	21.71845429498472
12	22.563966181947713	23.361256569876346	29.431531529348476	24.643245718827465
13	32.263575321254535	18.703277850338885	23.22119613549514	25.811950692911438
14	23.402996033236487	18.99557525340519	24.804811972501632	32.79661674085669
15	27.185657617664095	25.885615696461418	21.43681893190591	25.49190775396858
16	25.6175502024576	24.83570765655631	23.938278904426927	25.608463236559164
17	25.364266172981907	24.738477121443058	24.48719222446502	25.410064481110016
18	25.364447912299877	24.687287213548544	25.136789126657767	24.811475747493812
19	26.596277009491637	24.628343094754037	24.698555051262602	24.076824844491725
20	25.552245207534185	23.87400376563867	24.71854637623916	25.855204650587986
21	25.067485866739037	25.136789126657767	25.103591411242153	24.692133595361042
22	25.98012014180513	24.09923936037453	23.835838508865244	26.084801988955096
23	25.25607069901788	24.10905328354484	24.589511460481393	26.045364556955892
24	24.883081038773476	24.032419871134707	24.84867172790474	26.235827362187074
25	25.647961248331026	23.537180229670035	24.183324084821376	26.631534437177564
26	25.257827512424914	24.00958129684331	25.052340923574985	25.680250267156797
27	26.222560391975364	24.327564523515857	24.412860843415825	25.037014241092958
28	25.52807387824435	25.185979902054623	23.70450156174654	25.58144465795449
29	25.445261329023282	24.750290177111022	24.90604077261019	24.898407721255502
30	26.187908762016	24.22530586727214	24.288975208333838	25.29781016237802
31	25.435932044034224	24.23639196566823	24.845642739271927	25.482033251025616
32	24.9820078075211	24.223003835911207	24.623496712941538	26.17149164362616
33	25.190523385003843	23.979230830742537	24.541774599628283	26.28847118462534
34	25.722413788925536	24.2243971706823	25.269216509684284	24.78397253070789
35	25.487182531701396	23.937249048291772	24.873145956057858	25.702422463948977
36	25.073543844004664	23.893449872661318	25.433084794719385	25.599921488614637
37	24.19192641253856	23.595094492329387	25.921054863465308	26.29192423166674
38	25.141998987106202	23.070231342035818	25.642872547427903	26.144897123430077
39	23.98286561710191	23.42432011321148	26.648193874658027	25.944620395028583
40	25.291025227840525	22.73255968925	26.696900011873637	25.279515071035842
41	24.52990096418766	22.97003239806242	26.742880059319713	25.757186578430208
42	23.745392908289496	23.130750534919393	27.397444502870272	25.726412053920843
43	23.549175024655966	22.579474603747705	27.027241512168054	26.844108859428275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	76.0
2	101.0
3	310.5
4	520.0
5	520.0
6	681.0
7	842.0
8	768.5
9	695.0
10	941.5
11	1188.0
12	1188.0
13	2055.5
14	2923.0
15	4096.5
16	5270.0
17	4854.0
18	4438.0
19	4438.0
20	5010.5
21	5583.0
22	5364.5
23	5146.0
24	6440.0
25	7734.0
26	7734.0
27	9736.5
28	11739.0
29	14147.5
30	16556.0
31	20531.5
32	24507.0
33	24507.0
34	30014.0
35	35521.0
36	41259.5
37	46998.0
38	54593.0
39	62188.0
40	62188.0
41	70305.0
42	78422.0
43	88148.0
44	97874.0
45	109996.0
46	122118.0
47	122118.0
48	136388.0
49	150658.0
50	160568.5
51	170479.0
52	174356.5
53	178234.0
54	178234.0
55	166803.5
56	155373.0
57	140206.0
58	125039.0
59	115873.5
60	106708.0
61	106708.0
62	93853.5
63	80999.0
64	68111.5
65	55224.0
66	46247.0
67	37270.0
68	37270.0
69	30434.0
70	23598.0
71	19221.5
72	14845.0
73	12044.5
74	9244.0
75	9244.0
76	7511.5
77	5779.0
78	4553.5
79	3328.0
80	2631.0
81	1934.0
82	1934.0
83	1419.0
84	904.0
85	677.0
86	450.0
87	304.0
88	158.0
89	158.0
90	102.0
91	46.0
92	35.0
93	24.0
94	15.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1650716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.51917075914969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.92950860184132	54.98991805815563
2	8.935440922497664	11.708853592188367
3	2.7791937217634537	5.462714040869294
4	1.3199589941218794	3.4593047492378752
5	0.766271239398322	2.510272809098196
6	0.4973759099233371	1.9552594304252744
7	0.33909930467079713	1.5552253672924419
8	0.2640468722808747	1.3840105690712012
9	0.1953819601216634	1.1521137607621774
>10	0.9167875964237465	10.066830139055691
>50	0.036024557618930815	1.5977792864126297
>100	0.018689967698279367	2.4866884923775725
>500	0.0011101758199083404	0.4621880985575486
>1k	0.0011101758199083404	1.2088416064961562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2512	0.1521763889124477	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2380	0.14417985892182544	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2264	0.13715260529370285	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1832	0.11098214350621184	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.10607518192105728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0579772656229176E-5
2	0.0	0.0	0.0	1.2115954531245835E-4	6.0579772656229176E-5
3	0.0	0.0	0.0	1.2115954531245835E-4	6.0579772656229176E-5
4	0.0	0.0	0.0	1.8173931796868753E-4	6.0579772656229176E-5
5	0.0	0.0	0.0	1.8173931796868753E-4	6.0579772656229176E-5
6	0.0	0.0	0.0	3.0289886328114585E-4	1.2115954531245835E-4
7	0.0	0.0	0.0	3.0289886328114585E-4	1.2115954531245835E-4
8	0.0	0.0	0.0	3.0289886328114585E-4	1.2115954531245835E-4
9	0.0	0.0	0.0	4.240584085936042E-4	1.2115954531245835E-4
10	0.0	0.0	0.0	4.846381812498334E-4	1.2115954531245835E-4
11	0.0	0.0	0.0	4.846381812498334E-4	1.2115954531245835E-4
12	0.0	0.0	0.0	4.846381812498334E-4	1.2115954531245835E-4
13	0.0	0.0	0.0	4.846381812498334E-4	1.2115954531245835E-4
14	0.0	0.0	0.0	4.846381812498334E-4	2.423190906249167E-4
15	0.0	0.0	0.0	5.452179539060626E-4	3.6347863593737506E-4
16	0.0	0.0	0.0	5.452179539060626E-4	3.6347863593737506E-4
17	0.0	0.0	0.0	7.269572718747501E-4	3.6347863593737506E-4
18	6.0579772656229176E-5	0.0	0.0	9.086965898434376E-4	3.6347863593737506E-4
19	6.0579772656229176E-5	0.0	0.0	0.0012721752257808126	3.6347863593737506E-4
20	6.0579772656229176E-5	0.0	0.0	0.0015750740890619586	3.6347863593737506E-4
21	6.0579772656229176E-5	0.0	0.0	0.0021808718156242505	3.6347863593737506E-4
22	6.0579772656229176E-5	0.0	0.0	0.003513626814061292	3.6347863593737506E-4
23	6.0579772656229176E-5	0.0	0.0	0.0046646424945296465	6.057977265622917E-4
24	6.0579772656229176E-5	0.0	0.0	0.006906094082810126	6.057977265622917E-4
25	6.0579772656229176E-5	0.0	0.0	0.009329284989059293	6.663774992185209E-4
26	6.0579772656229176E-5	0.0	0.0	0.013812188165620251	7.269572718747501E-4
27	6.0579772656229176E-5	0.0	0.0	0.02641278087811592	7.269572718747501E-4
28	6.0579772656229176E-5	0.0	0.0	0.06500209606013391	7.269572718747501E-4
29	6.0579772656229176E-5	0.0	0.0	0.12509723053511324	7.269572718747501E-4
30	6.0579772656229176E-5	0.0	0.0	0.20354803612493003	7.269572718747501E-4
31	6.0579772656229176E-5	0.0	0.0	0.5627255082037128	7.269572718747501E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1050	0.0	19.38095	37
CGACTTA	180	1.0913936E-11	16.444445	19
TTAACCT	145	5.349466E-8	15.310345	4
ATAACAC	75	0.0041054147	14.8	3
TATAACA	150	8.107054E-8	14.8	4
TCTTATA	1705	0.0	14.53959	37
GTATATA	90	8.27671E-4	14.388888	1
TACTGTG	350	0.0	14.271429	7
CTTGTTA	715	0.0	14.230769	22
TACCTAT	80	0.0063001565	13.875	6
TAAGTCG	275	0.0	13.454546	20
GTATTAT	110	2.4573784E-4	13.454545	1
GTGTAAG	250	1.0913936E-11	13.32	1
GGGCGTC	390	0.0	13.282052	11
GTATAGA	195	1.0264557E-8	13.282052	1
CTCTTAT	2765	0.0	13.24774	37
TGTACTA	155	1.8887367E-6	13.129032	5
TGTACCG	155	1.8887367E-6	13.129032	5
TATAGTG	200	1.463377E-8	12.949999	5
GTCTATA	115	3.5786387E-4	12.869565	1
>>END_MODULE