FastQCFastQC Report
Thu 9 Feb 2017
SRR2727539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651363
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT27030.16368296976497596No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG22740.137704429613598No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG16840.10197636740074716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9600.021.39062737
TTACATA2600.019.21153834
TACTTAC5300.018.84905631
GTACTTA6000.018.80833430
GTATAGT1156.409937E-817.695651
GTATTAC1054.800404E-717.6190471
CTAGTAC1301.3942554E-817.0769233
GTTTGCG803.3836847E-416.18759
TAGATCG700.002593137315.8571425
TCTTATA17600.015.76704537
ACTTACA4600.015.68478132
TACATAT3100.015.51612935
GTCTTAG2650.015.3584911
AGTGTAC7000.015.32857227
TAGTACC1455.349466E-815.3103454
CGTTACG5250.015.15238224
TTAGAAC1752.239176E-914.83
ACGCTCG750.004105414714.813
TGTACTT8700.014.45976929
CTTACAT3850.014.41558433
Produced by FastQC (version 0.11.5)