##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727539.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1651363
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66198104232685	34.0	31.0	34.0	31.0	34.0
2	32.824196739299595	34.0	31.0	34.0	31.0	34.0
3	32.91910561154634	34.0	31.0	34.0	31.0	34.0
4	36.35397910695589	37.0	37.0	37.0	35.0	37.0
5	36.22165932021003	37.0	35.0	37.0	35.0	37.0
6	36.24721396809787	37.0	36.0	37.0	35.0	37.0
7	36.217590560040406	37.0	36.0	37.0	35.0	37.0
8	36.21325716998625	37.0	36.0	37.0	35.0	37.0
9	37.93735054012958	39.0	38.0	39.0	35.0	39.0
10	37.91651017977271	39.0	38.0	39.0	35.0	39.0
11	37.9967469296575	39.0	38.0	39.0	35.0	39.0
12	37.972324074113324	39.0	38.0	39.0	35.0	39.0
13	38.00347409987992	39.0	38.0	39.0	35.0	39.0
14	39.36685937616381	40.0	39.0	41.0	37.0	41.0
15	39.389932437628794	40.0	39.0	41.0	37.0	41.0
16	39.36608304776115	40.0	39.0	41.0	36.0	41.0
17	39.382488283920615	40.0	39.0	41.0	37.0	41.0
18	39.360685082565126	40.0	39.0	41.0	36.0	41.0
19	39.3997352490034	40.0	39.0	41.0	37.0	41.0
20	39.37751784435039	40.0	39.0	41.0	37.0	41.0
21	39.35344439714345	40.0	39.0	41.0	36.0	41.0
22	39.32593136699805	40.0	39.0	41.0	36.0	41.0
23	39.28958139427854	40.0	39.0	41.0	36.0	41.0
24	39.259071445829896	40.0	39.0	41.0	36.0	41.0
25	39.201465092774875	40.0	39.0	41.0	36.0	41.0
26	39.11216007625216	40.0	39.0	41.0	36.0	41.0
27	39.074259868968845	40.0	39.0	41.0	36.0	41.0
28	38.99242625637125	40.0	39.0	41.0	35.0	41.0
29	38.915902197154715	40.0	38.0	41.0	35.0	41.0
30	38.857617616478024	40.0	38.0	41.0	35.0	41.0
31	38.803746965385564	40.0	38.0	41.0	35.0	41.0
32	38.720674376257676	40.0	38.0	41.0	35.0	41.0
33	38.675123519177795	40.0	38.0	41.0	35.0	41.0
34	38.62964472378272	40.0	38.0	41.0	35.0	41.0
35	38.61746993241341	40.0	38.0	41.0	35.0	41.0
36	38.54911548823608	40.0	38.0	41.0	35.0	41.0
37	38.541309209422764	40.0	38.0	41.0	35.0	41.0
38	38.504779990831814	40.0	38.0	41.0	35.0	41.0
39	38.45554490442138	40.0	38.0	41.0	34.0	41.0
40	38.401864399287135	40.0	38.0	41.0	34.0	41.0
41	38.35135763608607	40.0	38.0	41.0	34.0	41.0
42	38.31083535237256	40.0	38.0	41.0	34.0	41.0
43	37.2147111204502	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	0.0
13	2.0
14	0.0
15	3.0
16	1.0
17	8.0
18	16.0
19	28.0
20	71.0
21	164.0
22	257.0
23	519.0
24	917.0
25	1404.0
26	2320.0
27	3543.0
28	5368.0
29	7716.0
30	10854.0
31	15284.0
32	21170.0
33	29341.0
34	43379.0
35	64625.0
36	99227.0
37	174338.0
38	416599.0
39	754204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.98306126514885	16.40220835758098	11.859899973537011	27.75483040373316
2	18.29391841769496	19.55972127266991	35.278736413496006	26.867623896139133
3	20.39866461825777	21.38094410496057	29.111588427256756	29.108802849524906
4	15.576587340275882	14.109556772193638	32.67609847138394	37.63775741614654
5	16.616455618782787	34.836798450734335	32.73290003469861	15.81384589578427
6	37.75977783200907	32.01743044987686	14.3922323559387	15.830559362175368
7	30.95667033838108	29.6320070148114	20.020007714839196	19.39131493196832
8	28.398904420166854	34.27635232229377	18.97511328520743	18.349629972331947
9	29.041403979621684	13.996861986129034	17.14504927142003	39.816684762829254
10	17.21850374508815	25.943417649541622	32.0758064701704	24.76227213519983
11	37.91019902952894	20.5177177882755	19.746657760892063	21.825425421303493
12	22.796199260853005	23.07923818082396	28.877054893442565	25.247507664880466
13	32.59271280754141	18.373125714939718	22.874800997721277	26.1593604797976
14	23.764248078708317	18.721262375383244	24.105541906897514	33.408947639010925
15	27.763489917116953	25.452247628171392	20.966195803103254	25.8180666516084
16	25.977026250436758	24.50272895783665	23.485205857222187	26.035038934504406
17	25.85536917080012	24.337653199205747	23.87391506289047	25.93306256710366
18	25.66249819088838	24.44931853263032	24.558077176247743	25.330106100233564
19	27.026825719118087	24.3540638854086	24.045894209813348	24.573216185659966
20	25.96866951724121	23.488839219481118	24.06829994374344	26.474191319534228
21	25.38490931430582	24.770507756320082	24.74029029353328	25.10429263584082
22	26.48557585461222	23.56962097370475	23.178005078229315	26.76679809345371
23	25.664738764281385	23.721979964429384	24.1725168845372	26.440764386752036
24	25.20312008928382	23.572285439361305	24.342437126180013	26.88215734517486
25	26.162388281680045	23.171404470125587	23.58784834103707	27.078358907157302
26	25.680786114258343	23.52359838509159	24.544573179852037	26.251042320798028
27	26.770492011750292	23.957966843147148	23.721616628203492	25.54992451689907
28	25.91477464373369	24.80968751267892	22.937052604424345	26.338485239163045
29	25.876987676240777	24.409412103819694	24.353761105220354	25.359839114719172
30	26.88881850931624	23.72585555083891	23.581368845008637	25.803957094836207
31	25.985140759481713	23.707991519732488	24.238704633687444	26.068163087098355
32	25.442316437996976	23.864347208941947	24.04147361906498	26.6518627339961
33	25.6801199978442	23.427738177493378	24.01894677305959	26.873195051602828
34	26.270117472657432	23.811179007886214	24.707105584901683	25.211597934554668
35	26.029104442814816	23.567622624462338	24.271828786281393	26.131444146441456
36	25.61865561963057	23.43615546672658	24.779106713666227	26.166082199976625
37	24.686213751912813	23.197564678389913	25.454185421376156	26.662036148321118
38	25.807348232944545	22.4687727652854	25.074014617016367	26.649864384753684
39	24.356970575215747	22.899750085232622	26.26939080020565	26.473888539345978
40	25.8812265988762	22.024170336867183	26.33140018275812	25.763202881498497
41	24.98463390544659	22.463262165859355	26.341815821233734	26.210288107460322
42	24.26819542402246	22.474767813012644	27.070910514526485	26.186126248438413
43	23.939133915438337	22.069829589254454	26.605113472931148	27.385923022376062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	35.0
2	47.0
3	145.0
4	243.0
5	243.0
6	312.5
7	382.0
8	359.0
9	336.0
10	486.5
11	637.0
12	637.0
13	1018.0
14	1399.0
15	2039.5
16	2680.0
17	2591.5
18	2503.0
19	2503.0
20	2943.5
21	3384.0
22	3523.0
23	3662.0
24	4806.0
25	5950.0
26	5950.0
27	7537.0
28	9124.0
29	11115.5
30	13107.0
31	16644.0
32	20181.0
33	20181.0
34	25251.0
35	30321.0
36	35813.5
37	41306.0
38	48684.5
39	56063.0
40	56063.0
41	64188.0
42	72313.0
43	82174.0
44	92035.0
45	105856.5
46	119678.0
47	119678.0
48	135938.5
49	152199.0
50	163880.0
51	175561.0
52	180522.0
53	185483.0
54	185483.0
55	173977.0
56	162471.0
57	147467.0
58	132463.0
59	124256.0
60	116049.0
61	116049.0
62	101718.0
63	87387.0
64	73361.0
65	59335.0
66	49740.0
67	40145.0
68	40145.0
69	32904.0
70	25663.0
71	20848.0
72	16033.0
73	12983.5
74	9934.0
75	9934.0
76	8006.0
77	6078.0
78	4829.0
79	3580.0
80	2761.5
81	1943.0
82	1943.0
83	1436.5
84	930.0
85	697.0
86	464.0
87	320.5
88	177.0
89	177.0
90	121.0
91	65.0
92	43.5
93	22.0
94	13.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1651363.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.48118960854722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09211579764028	52.74786357925364
2	9.119374115888231	11.578174347239568
3	2.939963615866336	5.598971632231228
4	1.4512106996005703	3.68498326333185
5	0.869948492110741	2.7612682588675845
6	0.5575131956748779	2.1234960530342404
7	0.3791488158595441	1.6848172508605108
8	0.2826336904201856	1.4353538313061787
9	0.21181256290464945	1.2101502120500145
>10	1.0318696907400966	11.03964038879239
>50	0.03909270046892554	1.6941645547088202
>100	0.023024022360090303	2.901213836249118
>500	0.0013373502715032066	0.5991273366031221
>1k	9.552501939308618E-4	0.9407754554717447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2703	0.16368296976497596	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2274	0.137704429613598	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	1684	0.10197636740074716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2111207529779945E-4
2	0.0	0.0	0.0	0.0	1.2111207529779945E-4
3	0.0	0.0	0.0	0.0	1.2111207529779945E-4
4	0.0	0.0	0.0	1.2111207529779945E-4	1.2111207529779945E-4
5	0.0	0.0	0.0	1.2111207529779945E-4	1.2111207529779945E-4
6	0.0	0.0	0.0	1.2111207529779945E-4	1.2111207529779945E-4
7	0.0	0.0	0.0	1.2111207529779945E-4	1.2111207529779945E-4
8	0.0	0.0	0.0	1.2111207529779945E-4	1.2111207529779945E-4
9	0.0	0.0	0.0	1.8166811294669918E-4	1.2111207529779945E-4
10	0.0	0.0	0.0	1.8166811294669918E-4	1.2111207529779945E-4
11	0.0	0.0	0.0	1.8166811294669918E-4	1.2111207529779945E-4
12	0.0	0.0	0.0	1.8166811294669918E-4	1.2111207529779945E-4
13	0.0	0.0	0.0	1.8166811294669918E-4	1.2111207529779945E-4
14	0.0	0.0	0.0	2.422241505955989E-4	1.2111207529779945E-4
15	0.0	0.0	0.0	3.0278018824449865E-4	1.8166811294669918E-4
16	0.0	0.0	0.0	6.055603764889973E-4	2.422241505955989E-4
17	0.0	0.0	0.0	7.872284894356965E-4	2.422241505955989E-4
18	0.0	0.0	0.0	9.083405647334959E-4	2.422241505955989E-4
19	0.0	0.0	0.0	0.0010294526400312955	2.422241505955989E-4
20	0.0	0.0	0.0	0.0012716767906268943	2.422241505955989E-4
21	0.0	0.0	0.0	0.0023616854683070894	2.422241505955989E-4
22	0.0	0.0	0.0	0.0032094699953916857	3.0278018824449865E-4
23	0.0	0.0	0.0	0.0043600347107207805	5.450043388400976E-4
24	0.0	0.0	0.0	0.006237271877836672	5.450043388400976E-4
25	0.0	0.0	0.0	0.008538401308494861	6.66116414137897E-4
26	0.0	0.0	0.0	0.011505647153290948	6.66116414137897E-4
27	0.0	0.0	0.0	0.021194613177114904	6.66116414137897E-4
28	0.0	0.0	0.0	0.058557688406486036	6.66116414137897E-4
29	0.0	0.0	0.0	0.11578314398469627	6.66116414137897E-4
30	0.0	0.0	0.0	0.18778427274923806	6.66116414137897E-4
31	0.0	0.0	0.0	0.5075201515354286	7.266724517867967E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	960	0.0	21.390627	37
TTACATA	260	0.0	19.211538	34
TACTTAC	530	0.0	18.849056	31
GTACTTA	600	0.0	18.808334	30
GTATAGT	115	6.409937E-8	17.69565	1
GTATTAC	105	4.800404E-7	17.619047	1
CTAGTAC	130	1.3942554E-8	17.076923	3
GTTTGCG	80	3.3836847E-4	16.1875	9
TAGATCG	70	0.0025931373	15.857142	5
TCTTATA	1760	0.0	15.767045	37
ACTTACA	460	0.0	15.684781	32
TACATAT	310	0.0	15.516129	35
GTCTTAG	265	0.0	15.358491	1
AGTGTAC	700	0.0	15.328572	27
TAGTACC	145	5.349466E-8	15.310345	4
CGTTACG	525	0.0	15.152382	24
TTAGAAC	175	2.239176E-9	14.8	3
ACGCTCG	75	0.0041054147	14.8	13
TGTACTT	870	0.0	14.459769	29
CTTACAT	385	0.0	14.415584	33
>>END_MODULE