FastQCFastQC Report
Thu 9 Feb 2017
SRR2727538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548494
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15600.020.39743637
TACTTAC8600.018.93023331
CCCGTTA650.001580647717.07692322
GTACTTA10300.017.06310730
ATACCGT1151.2435703E-616.0869566
ATTATAC2200.015.9772733
AATACCG700.002593655115.8571425
CGTTATG2350.015.74468124
TTATACT2950.015.6779664
CTCGTTA14950.015.46822822
AGTGTAC12100.015.28925627
TCGTTAT2700.015.07407523
CGTTACA3850.014.89610424
TTACATA5600.014.86607134
TAGTTCG750.004106229614.87
TCGTTAC12900.014.77131823
CTTACAT6850.014.313868533
ACTTACA8600.014.19767432
TCTCGTT16900.014.01183321
GATCTCG19050.013.98425219
Produced by FastQC (version 0.11.5)