##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727538.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2548494
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17268747738861	33.0	31.0	34.0	30.0	34.0
2	32.35619075422583	34.0	31.0	34.0	31.0	34.0
3	32.44751998631349	34.0	31.0	34.0	31.0	34.0
4	36.028734617385794	37.0	35.0	37.0	35.0	37.0
5	35.78617881776453	37.0	35.0	37.0	35.0	37.0
6	35.79090042982248	37.0	35.0	37.0	35.0	37.0
7	35.768411069439445	37.0	35.0	37.0	35.0	37.0
8	35.74207904746882	37.0	35.0	37.0	33.0	37.0
9	37.41079633697392	39.0	37.0	39.0	35.0	39.0
10	37.31745807523973	39.0	37.0	39.0	34.0	39.0
11	37.4129513351807	39.0	37.0	39.0	35.0	39.0
12	37.3775241377849	39.0	37.0	39.0	35.0	39.0
13	37.426123820577956	39.0	37.0	39.0	35.0	39.0
14	38.66319128081133	40.0	38.0	41.0	35.0	41.0
15	38.653305834740046	40.0	38.0	41.0	35.0	41.0
16	38.62497302328355	40.0	38.0	41.0	35.0	41.0
17	38.64373586910544	40.0	38.0	41.0	35.0	41.0
18	38.601195451117405	40.0	38.0	41.0	35.0	41.0
19	38.658697254143036	40.0	38.0	41.0	35.0	41.0
20	38.62849745771424	40.0	38.0	41.0	35.0	41.0
21	38.60515112062261	40.0	38.0	41.0	34.0	41.0
22	38.58553679153257	40.0	38.0	41.0	34.0	41.0
23	38.53588511489531	40.0	38.0	41.0	34.0	41.0
24	38.487686845642955	40.0	38.0	41.0	34.0	41.0
25	38.41225641496507	40.0	38.0	41.0	34.0	41.0
26	38.28778329476153	40.0	38.0	41.0	34.0	41.0
27	38.23313611882155	40.0	38.0	41.0	34.0	41.0
28	38.14080040996761	40.0	38.0	41.0	34.0	41.0
29	38.04605975136688	40.0	38.0	41.0	33.0	41.0
30	37.99137961478426	40.0	38.0	41.0	33.0	41.0
31	37.906075117304574	40.0	37.0	41.0	33.0	41.0
32	37.80112921592124	40.0	37.0	41.0	33.0	41.0
33	37.753722786869425	40.0	37.0	41.0	33.0	41.0
34	37.7013518572145	40.0	37.0	41.0	33.0	41.0
35	37.69680446569621	40.0	37.0	41.0	33.0	41.0
36	37.62114134857685	40.0	37.0	41.0	33.0	41.0
37	37.609712245741996	40.0	37.0	41.0	33.0	41.0
38	37.577662729439425	40.0	37.0	41.0	33.0	41.0
39	37.508718874755054	39.0	37.0	41.0	32.0	41.0
40	37.46176369259649	39.0	37.0	41.0	32.0	41.0
41	37.40437097360245	39.0	37.0	41.0	32.0	41.0
42	37.37272404800639	39.0	37.0	40.0	32.0	41.0
43	36.25173730053906	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	1.0
13	2.0
14	4.0
15	6.0
16	16.0
17	36.0
18	70.0
19	104.0
20	250.0
21	533.0
22	975.0
23	1702.0
24	2910.0
25	4614.0
26	7234.0
27	11007.0
28	15572.0
29	21818.0
30	30181.0
31	40689.0
32	54044.0
33	73245.0
34	103048.0
35	145742.0
36	220241.0
37	382554.0
38	783495.0
39	648394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49776770123845	16.70684725959724	11.969696612980057	27.825688426184247
2	18.81311080190889	19.8752282720697	35.141420776348696	26.170240149672708
3	20.3519411856571	21.779215489618576	29.366873141549483	28.501970183174848
4	15.897663482825545	14.450769748722186	32.925366902963084	36.72619986548919
5	16.376730728029965	35.580974489247374	32.63401836535616	15.408276417366492
6	37.463144900478476	32.42463980688203	14.60839225048205	15.503823042157446
7	30.808862018117367	30.225223210256726	20.047290674413986	18.91862409721192
8	28.256021006916242	34.762491102588434	18.921410056292068	18.06007783420326
9	29.767109516443828	13.795755454005384	17.04304581450849	39.39408921504229
10	17.06109569023902	26.143165336076912	32.26022898229307	24.535509991390995
11	38.068325842634906	20.537266322777295	20.045093298238097	21.349314536349702
12	22.601230373703057	23.02030140153361	28.865204312821614	25.51326391194172
13	32.317478479447075	18.764297659715893	22.90976553211426	26.008458328722767
14	23.79028555688183	19.13023927072224	23.75293016189169	33.32654501050424
15	27.673010020820136	25.984758057111378	20.905366071099245	25.436865850969237
16	25.591349243906404	25.075986052939502	23.606255302151	25.726409401003103
17	25.766511516213104	24.115026364590225	23.518909599159347	26.599552520037324
18	25.282657130054066	25.349873297720144	24.329192064018983	25.038277508206807
19	27.38711568479267	24.23898192422662	23.927817762176407	24.446084628804304
20	25.671828146348393	23.669900733531254	24.05687437364969	26.601396746470662
21	25.48320694496436	24.65954402874796	25.260290979692318	24.59695804659536
22	26.556468251445757	23.266329055512784	23.231759619602794	26.945443073438668
23	25.54179056336801	24.087716117832727	24.603550175123033	25.766943143676222
24	24.919030611804462	23.59566080987438	24.572080609175458	26.913227969145698
25	26.05409312323278	23.164464974216145	23.828896595401048	26.95254530715003
26	25.468727805519652	23.72848435193491	24.621482334272713	26.18130550827273
27	26.78111072657028	24.363094439304152	23.57698311237931	25.27881172174625
28	25.548147258733984	25.32578063750592	22.58871317727254	26.537358926487563
29	25.742654289160576	25.026662805562815	24.246594263121672	24.984088642154937
30	27.525746578175188	23.68728354863696	23.12075288386004	25.66621698932782
31	26.21022454830186	23.944533516657287	24.32946673604097	25.51577519899988
32	25.665079062379586	24.25118520977487	23.954853336911917	26.128882390933626
33	25.511458924368668	23.40162464577119	24.111887255767524	26.97502917409262
34	26.223291088776353	24.03015270979645	25.023523696740114	24.723032504687083
35	26.252131651084916	23.667428685333377	24.02002908384324	26.060410579738463
36	25.568551466081534	23.49932940787775	24.874769177404378	26.057349948636332
37	24.82340550929294	23.622853340051027	25.628233772573132	25.9255073780829
38	26.181776374596133	22.53715331485968	24.76474341316872	26.51632689737547
39	24.223757246436524	23.62379507269784	26.351798356205663	25.800649324659975
40	26.31471763323751	22.03214918300769	26.065119242972518	25.588013940782282
41	24.690111100909007	23.148298563779235	26.021054002873857	26.140536332437904
42	24.539316160838517	23.11973267349266	26.484111793082505	25.85683937258632
43	23.77651271692223	22.561245975073906	25.885169829711195	27.77707147829267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	69.0
2	98.0
3	198.0
4	298.0
5	298.0
6	360.0
7	422.0
8	423.5
9	425.0
10	543.0
11	661.0
12	661.0
13	1126.0
14	1591.0
15	2213.5
16	2836.0
17	2911.0
18	2986.0
19	2986.0
20	3847.5
21	4709.0
22	5497.5
23	6286.0
24	8157.0
25	10028.0
26	10028.0
27	13007.0
28	15986.0
29	19305.5
30	22625.0
31	28196.0
32	33767.0
33	33767.0
34	41011.5
35	48256.0
36	57293.0
37	66330.0
38	77968.0
39	89606.0
40	89606.0
41	102021.0
42	114436.0
43	129983.0
44	145530.0
45	168464.5
46	191399.0
47	191399.0
48	216672.0
49	241945.0
50	260358.5
51	278772.0
52	280134.0
53	281496.0
54	281496.0
55	259982.0
56	238468.0
57	219814.5
58	201161.0
59	188009.0
60	174857.0
61	174857.0
62	153671.5
63	132486.0
64	110257.5
65	88029.0
66	73239.5
67	58450.0
68	58450.0
69	47980.0
70	37510.0
71	30589.5
72	23669.0
73	19011.5
74	14354.0
75	14354.0
76	11598.0
77	8842.0
78	7006.0
79	5170.0
80	3989.5
81	2809.0
82	2809.0
83	2037.0
84	1265.0
85	918.5
86	572.0
87	401.0
88	230.0
89	230.0
90	145.0
91	60.0
92	41.0
93	22.0
94	14.0
95	6.0
96	6.0
97	5.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2548494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.71666258741715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77497063639329	47.197783883166565
2	9.256499708335202	10.685085408130162
3	3.1621414162838044	5.475247475320492
4	1.5754943714658032	3.6372910818506647
5	0.9822574327747708	2.834631041072202
6	0.6501077738613746	2.2513230617648308
7	0.44905099596619535	1.814240737310716
8	0.3259890403563145	1.5051999559553049
9	0.2612904026125192	1.3572729004425437
>10	1.4582530233289168	14.94925881308474
>50	0.06711498909852207	2.644205975513918
>100	0.03302425055905877	3.6709837066040976
>500	0.002514651458530107	0.9623779099709004
>1k	0.0012913075057240703	1.015098049812955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.923886028375974E-5	0.0
4	0.0	0.0	0.0	3.923886028375974E-5	3.923886028375974E-5
5	0.0	0.0	0.0	3.923886028375974E-5	3.923886028375974E-5
6	0.0	0.0	0.0	3.923886028375974E-5	1.1771658085127922E-4
7	0.0	0.0	0.0	3.923886028375974E-5	1.5695544113503897E-4
8	0.0	0.0	0.0	3.923886028375974E-5	1.961943014187987E-4
9	0.0	0.0	0.0	7.847772056751949E-5	2.3543316170255844E-4
10	0.0	0.0	0.0	1.1771658085127922E-4	2.3543316170255844E-4
11	0.0	0.0	0.0	1.5695544113503897E-4	2.3543316170255844E-4
12	0.0	0.0	0.0	1.961943014187987E-4	2.3543316170255844E-4
13	0.0	0.0	0.0	1.961943014187987E-4	2.3543316170255844E-4
14	0.0	0.0	0.0	1.961943014187987E-4	3.1391088227007794E-4
15	0.0	0.0	0.0	1.961943014187987E-4	3.531497425538377E-4
16	0.0	0.0	0.0	2.3543316170255844E-4	3.923886028375974E-4
17	0.0	0.0	0.0	4.3162746312135715E-4	3.923886028375974E-4
18	0.0	0.0	0.0	6.278217645401559E-4	4.708663234051169E-4
19	0.0	0.0	0.0	7.455383453914351E-4	4.708663234051169E-4
20	0.0	0.0	0.0	9.417326468102338E-4	4.708663234051169E-4
21	0.0	0.0	0.0	0.0014910766907828702	4.708663234051169E-4
22	0.0	0.0	0.0	0.002550525918444383	4.708663234051169E-4
23	0.0	0.0	0.0	0.0034137808446870976	5.101051836888767E-4
24	0.0	0.0	0.0	0.004590946653199889	5.101051836888767E-4
25	0.0	0.0	0.0	0.005768112461712682	5.101051836888767E-4
26	3.923886028375974E-5	0.0	0.0	0.008240160659589546	5.101051836888767E-4
27	3.923886028375974E-5	0.0	0.0	0.017225859664570527	5.101051836888767E-4
28	7.847772056751949E-5	0.0	0.0	0.04802836498732192	5.493440439726364E-4
29	7.847772056751949E-5	0.0	0.0	0.09550738593067121	5.493440439726364E-4
30	7.847772056751949E-5	0.0	0.0	0.15652381367191762	5.493440439726364E-4
31	7.847772056751949E-5	0.0	0.0	0.4585845601363001	5.493440439726364E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1560	0.0	20.397436	37
TACTTAC	860	0.0	18.930233	31
CCCGTTA	65	0.0015806477	17.076923	22
GTACTTA	1030	0.0	17.063107	30
ATACCGT	115	1.2435703E-6	16.086956	6
ATTATAC	220	0.0	15.977273	3
AATACCG	70	0.0025936551	15.857142	5
CGTTATG	235	0.0	15.744681	24
TTATACT	295	0.0	15.677966	4
CTCGTTA	1495	0.0	15.468228	22
AGTGTAC	1210	0.0	15.289256	27
TCGTTAT	270	0.0	15.074075	23
CGTTACA	385	0.0	14.896104	24
TTACATA	560	0.0	14.866071	34
TAGTTCG	75	0.0041062296	14.8	7
TCGTTAC	1290	0.0	14.771318	23
CTTACAT	685	0.0	14.3138685	33
ACTTACA	860	0.0	14.197674	32
TCTCGTT	1690	0.0	14.011833	21
GATCTCG	1905	0.0	13.984252	19
>>END_MODULE