FastQCFastQC Report
Thu 9 Feb 2017
SRR2727537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218851
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT17380.1425933112414889No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG16630.1364399750256594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9050.023.09944737
TCGTATA450.003825938320.5555572
TCGTTAC6450.018.93023523
GTATTAT701.2192743E-418.51
CTAGTAC609.2372514E-418.53
GTATACG500.00703512118.51
ACATATA1800.017.47222336
CGTTACG5350.017.28971924
GATACCG650.001580008117.0769235
TCTTATA15850.016.80757137
GTATAGG904.4463108E-516.4444451
ACTTACA4100.016.24390232
CATATAA1606.2937033E-1016.187537
TTAGAGT803.3827763E-416.18754
TTACATA2300.016.08695634
CGTTACA1504.671165E-916.03333324
GTTACGG8200.016.01829125
AGGGCGT6250.015.98399910
CTCGTTA7550.015.92715322
TTCGTTA1059.343947E-615.85714222
Produced by FastQC (version 0.11.5)