##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727537.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218851
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08916430310185	33.0	31.0	34.0	30.0	34.0
2	32.27625033740794	34.0	31.0	34.0	30.0	34.0
3	32.36623918756271	34.0	31.0	34.0	30.0	34.0
4	35.96749807810799	37.0	35.0	37.0	35.0	37.0
5	35.70863296662184	37.0	35.0	37.0	33.0	37.0
6	35.718563630829365	37.0	35.0	37.0	33.0	37.0
7	35.693097023344116	37.0	35.0	37.0	33.0	37.0
8	35.66701590268212	37.0	35.0	37.0	33.0	37.0
9	37.331502373957115	39.0	37.0	39.0	34.0	39.0
10	37.2277620480272	39.0	37.0	39.0	34.0	39.0
11	37.331199629815295	39.0	37.0	39.0	34.0	39.0
12	37.28897543670227	39.0	37.0	39.0	34.0	39.0
13	37.340465733711504	39.0	37.0	39.0	34.0	39.0
14	38.55552893667889	40.0	38.0	41.0	35.0	41.0
15	38.543758014720424	40.0	38.0	41.0	35.0	41.0
16	38.508089996234155	40.0	38.0	41.0	34.0	41.0
17	38.52244121717913	40.0	38.0	41.0	34.0	41.0
18	38.47908152842308	40.0	38.0	41.0	34.0	41.0
19	38.5350645813147	40.0	38.0	41.0	34.0	41.0
20	38.518639275842574	40.0	38.0	41.0	34.0	41.0
21	38.48476064752788	40.0	38.0	41.0	34.0	41.0
22	38.47045619193815	40.0	38.0	41.0	34.0	41.0
23	38.41465855957783	40.0	38.0	41.0	34.0	41.0
24	38.35765897554336	40.0	38.0	41.0	34.0	41.0
25	38.2855607453249	40.0	38.0	41.0	34.0	41.0
26	38.16036742801212	40.0	38.0	41.0	34.0	41.0
27	38.100298559873195	40.0	38.0	41.0	34.0	41.0
28	37.99779628518991	40.0	38.0	41.0	33.0	41.0
29	37.89580842941426	40.0	37.0	41.0	33.0	41.0
30	37.83415035964199	40.0	37.0	41.0	33.0	41.0
31	37.751922097122616	40.0	37.0	41.0	33.0	41.0
32	37.658039415810464	39.0	37.0	41.0	33.0	41.0
33	37.60965040025401	39.0	37.0	41.0	33.0	41.0
34	37.55840951847273	39.0	37.0	41.0	32.0	41.0
35	37.549079419879874	39.0	37.0	41.0	33.0	41.0
36	37.46920501357426	39.0	37.0	40.0	32.0	41.0
37	37.46633181578388	39.0	37.0	40.0	32.0	41.0
38	37.43649962136471	39.0	37.0	40.0	32.0	41.0
39	37.37789852902446	39.0	37.0	40.0	32.0	41.0
40	37.33343534197371	39.0	36.0	40.0	32.0	41.0
41	37.283772175598166	39.0	36.0	40.0	32.0	41.0
42	37.246677403554656	39.0	36.0	40.0	32.0	41.0
43	36.10208138648613	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	1.0
13	1.0
14	1.0
15	2.0
16	4.0
17	9.0
18	19.0
19	58.0
20	139.0
21	236.0
22	476.0
23	905.0
24	1457.0
25	2372.0
26	3626.0
27	5571.0
28	7826.0
29	11211.0
30	15460.0
31	20750.0
32	28102.0
33	37797.0
34	52502.0
35	73984.0
36	112535.0
37	190702.0
38	373595.0
39	279505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.331547498422694	16.556002333345095	12.146439556598796	27.96601061163341
2	18.7550406079168	20.022217645963288	34.58650811296869	26.636233633151225
3	20.68259368864611	21.50894572019057	29.378242295407723	28.430218295755594
4	16.408568397613816	14.423830312318733	32.0367296740947	37.13087161597275
5	16.8127195202695	34.92773111725715	32.44440870951412	15.815140652959222
6	38.04304217660731	31.719873881220924	14.237343202737659	15.999740739434106
7	30.83174235406953	29.997514052168807	19.669344325106188	19.50139926865548
8	28.53359434418153	34.35448631539048	18.91199170366189	18.199927636766102
9	29.639553973373285	13.698721172645383	16.684894215946002	39.97683063803533
10	17.24689892365843	26.11959952447018	31.910463214945878	24.723038336925516
11	38.43398413751968	20.3964225323686	19.196357881316093	21.973235448795627
12	22.886390543224724	22.955307908842016	28.515052291051163	25.643249256882093
13	33.004280260671734	18.186554386057033	22.431700019116366	26.37746533415487
14	23.91055182298739	18.892383072254116	23.748596013786756	33.44846909097174
15	28.404538372614862	25.407453413091506	20.615973568549396	25.57203464574423
16	25.96609429700595	24.64189634335944	23.547915208667835	25.84409415096677
17	26.189911646296387	23.97873078825878	23.390800023956988	26.44055754148784
18	25.82809547680561	24.84946888504009	24.119847298808466	25.202588339345827
19	27.573427761063495	24.289597333882483	23.774275936927484	24.36269896812654
20	26.1585706538371	23.160419116036334	23.58270206940799	27.098308160718577
21	25.478011668366356	24.92306278618141	24.6413220319793	24.957603513472932
22	27.10421536348577	23.2640412979109	22.462466700195513	27.169276638407812
23	25.93770690593026	23.732023028245454	23.79454092419828	26.535729141626007
24	25.14023453235875	23.338209510432367	24.217480233432962	27.304075723775917
25	26.40708339247373	22.670367419807672	23.227531503030313	27.695017684688285
26	25.700270172482114	23.3716016149636	24.363191235023805	26.56493697753048
27	27.383412738718675	23.848198016000318	23.246155600643554	25.52223364463745
28	25.96100753906753	25.153279605136312	22.11107018003021	26.774642675765946
29	26.17243617144343	24.58602405051971	23.930570676809555	25.310969101227304
30	27.682382834325114	23.337635199052222	22.86284377663882	26.117138189983848
31	26.228062330834533	23.60222865633289	24.048058376290456	26.12165063654212
32	25.73079072011263	23.677135269200257	23.55718623523302	27.0348877754541
33	25.8742044761829	23.03956759275744	23.6025568342644	27.48367109679526
34	26.59225779032876	23.636687339141535	24.74018563384696	25.030869236682747
35	26.431778781819926	23.05728920105903	23.918920360240918	26.59201165688013
36	25.600832259234313	23.06877542866191	24.866944359893047	26.46344795221073
37	24.591028763975253	22.770707822367132	25.710033465944566	26.92822994771305
38	26.030253082616333	21.741295695700295	25.044734754289085	27.18371646739429
39	24.081122302890183	22.540080780997844	26.711550468432975	26.667246447679005
40	26.18662986698128	21.178962810056355	26.71901651637485	25.915390806587517
41	24.82321465051922	21.91613248871273	26.699079707035562	26.561573153732493
42	23.96248598064899	21.929341650456045	27.631843432872433	26.47632893602253
43	23.442077825755568	21.30974171576345	26.9733544132958	28.27482604518518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	19.0
2	27.0
3	61.0
4	95.0
5	95.0
6	134.5
7	174.0
8	149.5
9	125.0
10	165.5
11	206.0
12	206.0
13	407.5
14	609.0
15	883.5
16	1158.0
17	1113.5
18	1069.0
19	1069.0
20	1270.5
21	1472.0
22	1638.5
23	1805.0
24	2378.5
25	2952.0
26	2952.0
27	3950.5
28	4949.0
29	6297.0
30	7645.0
31	9900.5
32	12156.0
33	12156.0
34	15492.5
35	18829.0
36	23049.0
37	27269.0
38	32468.0
39	37667.0
40	37667.0
41	43931.0
42	50195.0
43	58128.0
44	66061.0
45	78216.5
46	90372.0
47	90372.0
48	103770.5
49	117169.0
50	128394.5
51	139620.0
52	143796.0
53	147972.0
54	147972.0
55	135821.0
56	123670.0
57	111380.5
58	99091.0
59	92738.0
60	86385.0
61	86385.0
62	75491.0
63	64597.0
64	53559.5
65	42522.0
66	35250.0
67	27978.0
68	27978.0
69	22973.5
70	17969.0
71	14614.5
72	11260.0
73	9000.5
74	6741.0
75	6741.0
76	5502.0
77	4263.0
78	3334.5
79	2406.0
80	1858.0
81	1310.0
82	1310.0
83	956.0
84	602.0
85	449.5
86	297.0
87	203.0
88	109.0
89	109.0
90	69.0
91	29.0
92	20.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1218851.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.91080948698792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6295700841561	58.15173420437806
2	8.021328657487299	10.89469844582273
3	2.5208835629783963	5.135857301529156
4	1.2108297845095972	3.2891372326800763
5	0.7083404168400302	2.405198554997845
6	0.4340179799251025	1.7684707409172589
7	0.31075253380620094	1.477241928463818
8	0.2196225357953328	1.1931795349956824
9	0.1639904760241464	1.0023053377460642
>10	0.715534771452158	8.14459245504177
>50	0.03459132895267642	1.6255257051679757
>100	0.02836522811065013	3.6202136472251873
>500	0.001448426641595723	0.6699396229622311
>1k	7.242133207978615E-4	0.6219052880722437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1738	0.1425933112414889	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1663	0.1364399750256594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.461334486331799E-4
2	0.0	0.0	0.0	8.204448287772664E-5	2.461334486331799E-4
3	0.0	0.0	0.0	1.640889657554533E-4	2.461334486331799E-4
4	0.0	0.0	0.0	1.640889657554533E-4	2.461334486331799E-4
5	0.0	0.0	0.0	1.640889657554533E-4	2.461334486331799E-4
6	0.0	0.0	0.0	1.640889657554533E-4	2.461334486331799E-4
7	0.0	0.0	0.0	1.640889657554533E-4	3.281779315109066E-4
8	0.0	0.0	0.0	1.640889657554533E-4	3.281779315109066E-4
9	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
10	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
11	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
12	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
13	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
14	0.0	0.0	0.0	2.461334486331799E-4	3.281779315109066E-4
15	0.0	0.0	0.0	3.281779315109066E-4	3.281779315109066E-4
16	0.0	0.0	0.0	4.1022241438863323E-4	4.1022241438863323E-4
17	0.0	0.0	0.0	4.1022241438863323E-4	4.1022241438863323E-4
18	0.0	0.0	0.0	5.743113801440865E-4	4.1022241438863323E-4
19	0.0	0.0	0.0	9.024893116549931E-4	4.1022241438863323E-4
20	0.0	0.0	0.0	0.0010665782774104465	4.1022241438863323E-4
21	0.0	0.0	0.0	0.0015588451746768063	4.1022241438863323E-4
22	0.0	0.0	0.0	0.002789512417842706	4.1022241438863323E-4
23	0.0	0.0	0.0	0.003692001729497699	4.1022241438863323E-4
24	0.0	0.0	0.0	0.004922668972663599	4.1022241438863323E-4
25	0.0	0.0	0.0	0.006317425181584952	4.922668972663598E-4
26	0.0	0.0	0.0	0.009435115530938565	4.922668972663598E-4
27	0.0	0.0	0.0	0.023300633137274367	5.743113801440865E-4
28	0.0	0.0	0.0	0.07334776769268762	5.743113801440865E-4
29	0.0	0.0	0.0	0.14882869194019613	5.743113801440865E-4
30	0.0	0.0	0.0	0.24441051449274767	5.743113801440865E-4
31	0.0	0.0	0.0	0.6660371120013849	5.743113801440865E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	905	0.0	23.099447	37
TCGTATA	45	0.0038259383	20.555557	2
TCGTTAC	645	0.0	18.930235	23
GTATTAT	70	1.2192743E-4	18.5	1
CTAGTAC	60	9.2372514E-4	18.5	3
GTATACG	50	0.007035121	18.5	1
ACATATA	180	0.0	17.472223	36
CGTTACG	535	0.0	17.289719	24
GATACCG	65	0.0015800081	17.076923	5
TCTTATA	1585	0.0	16.807571	37
GTATAGG	90	4.4463108E-5	16.444445	1
ACTTACA	410	0.0	16.243902	32
CATATAA	160	6.2937033E-10	16.1875	37
TTAGAGT	80	3.3827763E-4	16.1875	4
TTACATA	230	0.0	16.086956	34
CGTTACA	150	4.671165E-9	16.033333	24
GTTACGG	820	0.0	16.018291	25
AGGGCGT	625	0.0	15.983999	10
CTCGTTA	755	0.0	15.927153	22
TTCGTTA	105	9.343947E-6	15.857142	22
>>END_MODULE