FastQCFastQC Report
Thu 9 Feb 2017
SRR2727536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927846
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG451.3229437E-424.6666664
CTAGACG400.001930907723.1250024
TATTAGG450.003825260820.5555552
CTTATAC5650.019.31858437
TACTTAC2200.018.531
TATATAA609.234987E-418.52
GTATAAA902.151306E-618.51
TCTTATA10050.016.93532237
TAAGAGT1107.80361E-716.8181824
ATAGGAC1005.875332E-616.6500023
TAATGCA803.3816852E-416.18754
TATAGGA700.002591988315.8571432
GTACTTA2600.015.65384530
GTTTAGG1101.4511888E-515.1363641
GTATATA750.00410361314.81
TAAGACT750.00410361314.84
CTAATAC1001.0930028E-414.83
ACTTACA2251.8189894E-1214.79999832
CTCTTAT16800.014.53571537
GCCGTCA908.2718953E-414.3888888
Produced by FastQC (version 0.11.5)