##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727536.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	927846
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52386818502208	34.0	31.0	34.0	31.0	34.0
2	32.70402308141653	34.0	31.0	34.0	31.0	34.0
3	32.79781881907127	34.0	31.0	34.0	31.0	34.0
4	36.26848636519422	37.0	37.0	37.0	35.0	37.0
5	36.11615397382755	37.0	35.0	37.0	35.0	37.0
6	36.13720703651253	37.0	35.0	37.0	35.0	37.0
7	36.113076954580826	37.0	35.0	37.0	35.0	37.0
8	36.097211175130354	37.0	35.0	37.0	35.0	37.0
9	37.828374536291584	39.0	38.0	39.0	35.0	39.0
10	37.77743181519347	39.0	37.0	39.0	35.0	39.0
11	37.86502932598729	39.0	38.0	39.0	35.0	39.0
12	37.8256424018641	39.0	37.0	39.0	35.0	39.0
13	37.868910357968886	39.0	38.0	39.0	35.0	39.0
14	39.19469933588117	40.0	39.0	41.0	36.0	41.0
15	39.205100846476675	40.0	39.0	41.0	36.0	41.0
16	39.18834375532146	40.0	39.0	41.0	36.0	41.0
17	39.19390825632702	40.0	39.0	41.0	36.0	41.0
18	39.164095119233146	40.0	39.0	41.0	36.0	41.0
19	39.20187725118177	40.0	39.0	41.0	36.0	41.0
20	39.171309678545796	40.0	39.0	41.0	36.0	41.0
21	39.14548211664436	40.0	39.0	41.0	36.0	41.0
22	39.118831142236964	40.0	39.0	41.0	36.0	41.0
23	39.07496287099368	40.0	39.0	41.0	36.0	41.0
24	39.03424921808145	40.0	39.0	41.0	36.0	41.0
25	38.96638558553898	40.0	38.0	41.0	35.0	41.0
26	38.862914750939275	40.0	38.0	41.0	35.0	41.0
27	38.81606322600949	40.0	38.0	41.0	35.0	41.0
28	38.73128838190821	40.0	38.0	41.0	35.0	41.0
29	38.65364187591475	40.0	38.0	41.0	35.0	41.0
30	38.59113258019111	40.0	38.0	41.0	35.0	41.0
31	38.5240923601546	40.0	38.0	41.0	34.0	41.0
32	38.43892628733648	40.0	38.0	41.0	34.0	41.0
33	38.39283243124398	40.0	38.0	41.0	34.0	41.0
34	38.35051183062706	40.0	38.0	41.0	34.0	41.0
35	38.33603205704395	40.0	38.0	41.0	34.0	41.0
36	38.26570249804386	40.0	38.0	41.0	34.0	41.0
37	38.257013556128925	40.0	38.0	41.0	34.0	41.0
38	38.22585536823999	40.0	38.0	41.0	34.0	41.0
39	38.17388553703955	40.0	38.0	41.0	34.0	41.0
40	38.12972519146496	40.0	38.0	41.0	34.0	41.0
41	38.07958324980654	40.0	38.0	41.0	33.0	41.0
42	38.05437755834481	40.0	38.0	41.0	33.0	41.0
43	36.915067802199935	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	2.0
14	0.0
15	1.0
16	0.0
17	7.0
18	9.0
19	28.0
20	30.0
21	125.0
22	211.0
23	384.0
24	619.0
25	1091.0
26	1618.0
27	2505.0
28	3711.0
29	5271.0
30	7421.0
31	10382.0
32	14164.0
33	19595.0
34	28364.0
35	40697.0
36	63194.0
37	107113.0
38	249738.0
39	371564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.890365427021294	16.05083171129692	11.577891158662105	28.480911703019686
2	19.698743110386854	19.197474580911056	33.29992261646868	27.803859692233406
3	20.851951724747426	20.58229490669788	28.69269253733917	29.873060831215525
4	16.33202061549007	13.961907471714055	31.727786723227773	37.9782851895681
5	17.229691134089062	34.11029416519551	32.58590326411926	16.07411143659616
6	39.04915255333321	31.111520661833968	13.990144916289987	15.84918186854284
7	31.671096281063882	28.544392065062524	19.551520403170354	20.232991250703243
8	29.162274774046555	32.90341285083947	18.547151143616507	19.38716123149747
9	29.042211746345835	12.970579169388024	16.64780577811404	41.3394033061521
10	17.492558032259666	25.332759962321333	32.0532717713931	25.121410234025905
11	39.39985730390604	19.63795716099439	19.10047572549755	21.861709809602026
12	23.267977660085833	22.47204816316501	28.43930997169789	25.82066420505127
13	33.66690161944978	17.710913233446067	21.809653757196777	26.812531389907377
14	24.461818017214064	18.13328936051888	23.005972973963353	34.3989196483037
15	28.120614843411513	25.09834606173869	20.101827242882976	26.679211851966812
16	26.668649754377345	23.943305246775864	22.54016291496649	26.847882083880297
17	26.634700154982617	23.34622340345273	23.002200796252826	27.016875645311828
18	26.677595204376587	23.719453443782694	23.393429513087302	26.209521838753414
19	28.025448188600265	23.259032210086588	23.267331001049744	25.448188600263403
20	27.10342017964188	22.505997762559733	23.193288541417434	27.19729351638095
21	26.581350784505187	23.651769798005272	23.763749587754866	26.00312982973468
22	27.688538830797356	22.615498692670982	22.44715179027554	27.248810686256125
23	26.810483636293093	23.023324991431767	23.267331001049744	26.898860371225396
24	26.42852369897591	22.71756304386719	23.393537289593315	27.460375967563582
25	27.08962478687196	22.242484205353044	22.846571521567157	27.82131948620784
26	26.588895139926237	22.83719496554385	23.49441609922336	27.079493795306547
27	27.537005063340253	23.07397994925882	22.842476014338587	26.54653897306234
28	26.624353610405176	23.910756741959332	22.227072164992897	27.237817482642594
29	26.693869456784853	23.67192400463008	23.33803238899559	26.29617414958948
30	27.768508998260486	22.79462324566792	22.624875248694288	26.8119925073773
31	26.787096134487836	22.96760453782201	23.458957628744425	26.78634169894573
32	26.26987668212182	23.103079605882872	23.387717358268507	27.239326353726806
33	26.34920019054886	22.668740286642393	23.344606755862504	27.63745276694624
34	26.823740146532938	22.932469396861116	24.181491325069032	26.062299131536914
35	26.725340196541236	22.499746725210866	23.724626716071416	27.050286362176486
36	26.193032033333118	22.351985135464293	24.40060096179754	27.054381869405052
37	25.558551742422775	21.98716166260349	25.072048594271035	27.382238000702703
38	26.066286862259467	21.218499621704463	24.897342878020705	27.817870638015364
39	24.82233042983426	21.62352373130886	26.407507280302983	27.1466385585539
40	26.01746410503467	20.58057048260164	26.55225112788114	26.849714284482555
41	24.947674506329715	20.959404901244387	26.942294303149446	27.150626289276452
42	24.05517726001944	20.91467765124816	27.689293266339458	27.340851822392942
43	23.507888162475236	20.474087294658812	27.468135875996662	28.54988866686929
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	10.0
2	15.0
3	28.0
4	41.0
5	41.0
6	59.0
7	77.0
8	65.0
9	53.0
10	79.5
11	106.0
12	106.0
13	170.5
14	235.0
15	354.5
16	474.0
17	463.0
18	452.0
19	452.0
20	548.5
21	645.0
22	740.0
23	835.0
24	1188.0
25	1541.0
26	1541.0
27	2117.0
28	2693.0
29	3504.0
30	4315.0
31	5853.0
32	7391.0
33	7391.0
34	9695.5
35	12000.0
36	14706.0
37	17412.0
38	20996.0
39	24580.0
40	24580.0
41	29338.5
42	34097.0
43	40580.5
44	47064.0
45	55043.0
46	63022.0
47	63022.0
48	73116.0
49	83210.0
50	91129.5
51	99049.0
52	101943.0
53	104837.0
54	104837.0
55	100625.0
56	96413.0
57	90876.0
58	85339.0
59	80347.5
60	75356.0
61	75356.0
62	66484.0
63	57612.0
64	48540.0
65	39468.0
66	32970.0
67	26472.0
68	26472.0
69	21800.5
70	17129.0
71	13945.0
72	10761.0
73	8671.0
74	6581.0
75	6581.0
76	5324.0
77	4067.0
78	3219.5
79	2372.0
80	1798.5
81	1225.0
82	1225.0
83	876.5
84	528.0
85	381.0
86	234.0
87	167.5
88	101.0
89	101.0
90	60.0
91	19.0
92	16.5
93	14.0
94	9.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	927846.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.7094621949884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.10475436791549	65.07549353453534
2	7.532450023110597	11.254905804744414
3	2.33914522924101	5.242688462177061
4	1.0416213074417948	3.1127587075926866
5	0.580107166704487	2.1669747219975353
6	0.351061866273184	1.573658593586311
7	0.23708773052775906	1.239888778853145
8	0.16446877859847028	0.9829899197566667
9	0.11880580738618995	0.798832617791727
>10	0.49874909305178017	6.051371647134882
>50	0.020928278189029214	1.0729414285146746
>100	0.010676080253380143	1.3727413500878742
>500	1.4427130675358468E-4	0.05475443322766083
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.077765060150068E-4	1.077765060150068E-4
2	0.0	0.0	0.0	2.155530120300136E-4	1.077765060150068E-4
3	0.0	0.0	0.0	3.233295180450204E-4	1.077765060150068E-4
4	0.0	0.0	0.0	4.311060240600272E-4	1.077765060150068E-4
5	0.0	0.0	0.0	5.38882530075034E-4	1.077765060150068E-4
6	0.0	0.0	0.0	5.38882530075034E-4	2.155530120300136E-4
7	0.0	0.0	0.0	6.466590360900408E-4	2.155530120300136E-4
8	1.077765060150068E-4	0.0	0.0	6.466590360900408E-4	2.155530120300136E-4
9	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	3.233295180450204E-4
10	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	3.233295180450204E-4
11	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	3.233295180450204E-4
12	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	3.233295180450204E-4
13	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	3.233295180450204E-4
14	1.077765060150068E-4	0.0	0.0	8.622120481200544E-4	4.311060240600272E-4
15	1.077765060150068E-4	0.0	0.0	9.699885541350612E-4	4.311060240600272E-4
16	1.077765060150068E-4	0.0	0.0	0.0012933180721800817	6.466590360900408E-4
17	1.077765060150068E-4	0.0	0.0	0.0015088710842100952	6.466590360900408E-4
18	1.077765060150068E-4	0.0	0.0	0.002047753614285129	6.466590360900408E-4
19	1.077765060150068E-4	0.0	0.0	0.0022633066263151426	6.466590360900408E-4
20	1.077765060150068E-4	0.0	0.0	0.0022633066263151426	6.466590360900408E-4
21	1.077765060150068E-4	0.0	0.0	0.002802189156390177	7.544355421050476E-4
22	1.077765060150068E-4	0.0	0.0	0.003772177710525238	7.544355421050476E-4
23	1.077765060150068E-4	0.0	0.0	0.004957719276690313	0.001077765060150068
24	1.077765060150068E-4	0.0	0.0	0.006789919878945429	0.001077765060150068
25	1.077765060150068E-4	0.0	0.0	0.00808323795112551	0.001077765060150068
26	1.077765060150068E-4	0.0	0.0	0.011100980119545701	0.001077765060150068
27	1.077765060150068E-4	0.0	0.0	0.030824080720291944	0.001077765060150068
28	1.077765060150068E-4	0.0	0.0	0.08676008734208047	0.001077765060150068
29	1.077765060150068E-4	0.0	0.0	0.17352017468416095	0.001077765060150068
30	1.077765060150068E-4	0.0	0.0	0.269010159013457	0.001077765060150068
31	1.077765060150068E-4	0.0	0.0	0.5985907144073478	0.001077765060150068
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	45	1.3229437E-4	24.666666	4
CTAGACG	40	0.0019309077	23.125002	4
TATTAGG	45	0.0038252608	20.555555	2
CTTATAC	565	0.0	19.318584	37
TACTTAC	220	0.0	18.5	31
TATATAA	60	9.234987E-4	18.5	2
GTATAAA	90	2.151306E-6	18.5	1
TCTTATA	1005	0.0	16.935322	37
TAAGAGT	110	7.80361E-7	16.818182	4
ATAGGAC	100	5.875332E-6	16.650002	3
TAATGCA	80	3.3816852E-4	16.1875	4
TATAGGA	70	0.0025919883	15.857143	2
GTACTTA	260	0.0	15.653845	30
GTTTAGG	110	1.4511888E-5	15.136364	1
GTATATA	75	0.004103613	14.8	1
TAAGACT	75	0.004103613	14.8	4
CTAATAC	100	1.0930028E-4	14.8	3
ACTTACA	225	1.8189894E-12	14.799998	32
CTCTTAT	1680	0.0	14.535715	37
GCCGTCA	90	8.2718953E-4	14.388888	8
>>END_MODULE