FastQCFastQC Report
Thu 9 Feb 2017
SRR2727535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753481
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15000.019.60999937
TATACCG1005.883785E-616.6500025
CATATAC2250.015.6222223
TCTTATA26550.015.60828637
CGTTACG6050.014.67768624
TTAGACT2700.014.3888894
TCTATAC2650.013.9622643
GTCTTAG3450.013.9420281
TACGGAT13600.013.7389727
TCGTTAC7300.013.68493223
GGACCGT1907.1377144E-913.6315796
GTTACGG10950.013.17808225
GTCTAGA2950.013.1694921
TATACTA2408.185452E-1113.1041665
TTAGACG850.009409090513.0588234
ACGGATG15200.012.65789528
TACTTAC7450.012.41610731
GTACTTA7600.012.41447430
TTACGGA15500.012.41290326
CTCGTTA8650.012.40462422
Produced by FastQC (version 0.11.5)