##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727535.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2753481
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19641174208211	33.0	31.0	34.0	30.0	34.0
2	32.38572919152157	34.0	31.0	34.0	31.0	34.0
3	32.47773491082742	34.0	31.0	34.0	31.0	34.0
4	36.050224061833006	37.0	35.0	37.0	35.0	37.0
5	35.80878458939793	37.0	35.0	37.0	35.0	37.0
6	35.8148612610728	37.0	35.0	37.0	35.0	37.0
7	35.7932885681797	37.0	35.0	37.0	35.0	37.0
8	35.769339973655164	37.0	35.0	37.0	35.0	37.0
9	37.43585446930631	39.0	37.0	39.0	35.0	39.0
10	37.35113552626657	39.0	37.0	39.0	34.0	39.0
11	37.44272795054696	39.0	37.0	39.0	35.0	39.0
12	37.40590692290958	39.0	37.0	39.0	35.0	39.0
13	37.45289072268884	39.0	37.0	39.0	35.0	39.0
14	38.70657869075545	40.0	38.0	41.0	35.0	41.0
15	38.70010506700427	40.0	38.0	41.0	35.0	41.0
16	38.68154964570302	40.0	38.0	41.0	35.0	41.0
17	38.690222304058025	40.0	38.0	41.0	35.0	41.0
18	38.65707190280231	40.0	38.0	41.0	35.0	41.0
19	38.7010693010048	40.0	38.0	41.0	35.0	41.0
20	38.67808385095085	40.0	38.0	41.0	35.0	41.0
21	38.65194493806204	40.0	38.0	41.0	35.0	41.0
22	38.624326806685794	40.0	38.0	41.0	35.0	41.0
23	38.57455962107601	40.0	38.0	41.0	34.0	41.0
24	38.525865985637815	40.0	38.0	41.0	34.0	41.0
25	38.458198186223186	40.0	38.0	41.0	34.0	41.0
26	38.335756084752354	40.0	38.0	41.0	34.0	41.0
27	38.27958464213118	40.0	38.0	41.0	34.0	41.0
28	38.20204679095298	40.0	38.0	41.0	34.0	41.0
29	38.112564059821004	40.0	38.0	41.0	34.0	41.0
30	38.05327619838307	40.0	38.0	41.0	34.0	41.0
31	37.97699021711063	40.0	38.0	41.0	33.0	41.0
32	37.87508975002915	40.0	37.0	41.0	33.0	41.0
33	37.821738374079935	40.0	37.0	41.0	33.0	41.0
34	37.77193341809876	40.0	37.0	41.0	33.0	41.0
35	37.75839382948348	40.0	37.0	41.0	33.0	41.0
36	37.69220706443952	40.0	37.0	41.0	33.0	41.0
37	37.66502401868762	40.0	37.0	41.0	33.0	41.0
38	37.62917848352685	40.0	37.0	41.0	33.0	41.0
39	37.574118361448654	40.0	37.0	41.0	33.0	41.0
40	37.514828320950826	39.0	37.0	41.0	33.0	41.0
41	37.46581545323901	39.0	37.0	41.0	32.0	41.0
42	37.421035046183356	39.0	37.0	41.0	32.0	41.0
43	36.32239663175449	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	1.0
13	5.0
14	5.0
15	8.0
16	13.0
17	30.0
18	64.0
19	142.0
20	250.0
21	531.0
22	986.0
23	1836.0
24	3101.0
25	4932.0
26	7581.0
27	11392.0
28	16391.0
29	23306.0
30	32162.0
31	43197.0
32	56975.0
33	76820.0
34	106985.0
35	152012.0
36	232513.0
37	405851.0
38	845134.0
39	731252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17135291654455	17.22859173533429	12.101445406741503	27.49860994137966
2	18.64737762853639	19.86754221292974	35.13447160158359	26.350608556950274
3	20.251964694871692	21.77204055520993	29.264156898122778	28.7118378517956
4	15.581186142196005	14.623162462352202	33.27696831755876	36.51868307789304
5	16.230110176899714	35.201405057815904	33.148912231462646	15.419572533821732
6	37.144472760117104	32.89861088563894	14.696124650941844	15.260791703302113
7	30.678148859570847	29.688020364041005	20.377369591437166	19.256461184950975
8	27.952035986447697	34.47468858510373	19.04520132879072	18.52807409965785
9	28.521496970561987	13.946637002398054	17.615556453812463	39.91630957322749
10	16.866831476229542	26.16393575986179	32.72759100208064	24.241641761828028
11	37.77106143096684	20.599524747038387	20.42465519101094	21.204758630983836
12	22.414899539891504	23.336496601937693	29.49063385583558	24.757970002335224
13	31.879755117249765	18.81770747646343	23.344304899870384	25.958232506416422
14	23.408877707890486	18.996281434300798	24.320777953434217	33.2740629043745
15	26.658146542503836	26.243289857456798	21.330744610186162	25.767818989853208
16	25.69943282702877	24.9999909205838	23.608370640654503	25.69220561173293
17	25.456612920154527	24.720054360280677	24.152954024378595	25.6703786951862
18	25.35851890752106	24.947838753926394	24.714643028225	24.978999310327545
19	26.64510850083948	24.482246291149277	24.45943879765286	24.413206410358377
20	25.7179185184136	23.940096190966997	24.524229511661783	25.81775577895762
21	25.293147110875285	24.647927477981508	25.176785312845812	24.88214009829739
22	26.14436053853286	23.764645552302703	23.911441553437268	26.17955235572717
23	25.494455926879468	24.105850013128833	24.655372599266165	25.744321460725534
24	25.1596433750587	24.026096421220995	24.689765427834804	26.124494775885506
25	25.905717163110985	23.650535449491024	24.21549304316972	26.22825434422827
26	25.39647086724041	24.071892996537837	24.75688047239113	25.774755663830618
27	26.122497304321328	24.508322374477977	24.073236750135553	25.295943571065138
28	25.527359731191172	24.995088035835366	23.57731903724776	25.900233195725704
29	25.508147686510274	24.90796195797247	24.514060565516886	25.069829790000362
30	26.54654235856358	24.11307722842467	23.849774158601424	25.490606254410324
31	25.808567409762407	24.097351679564884	24.612190895815154	25.481890014857555
32	25.310506954651224	24.39101631716362	24.453918512602772	25.844558215582385
33	25.31166911992492	23.875632335941305	24.50665176189703	26.30604678223674
34	25.78100230217677	24.07835754087281	25.154704172645463	24.98593598430496
35	25.744103554736714	23.842038495998338	24.623994136876195	25.789863812388752
36	25.342212276024423	23.75008216871662	25.13770750551756	25.7699980497414
37	24.780160095529986	23.581422933370522	25.698851744391916	25.939565226707572
38	25.47793138939401	22.939762431627457	25.409908403217603	26.172397775760935
39	24.415385470246573	23.428997694191462	26.406392490088	25.749224345473966
40	25.549404553726717	22.630626468822555	26.38812470469199	25.431844272758735
41	24.52477427663383	23.0693438596453	26.474814970577242	25.931066893143623
42	24.14431042015543	23.245557169270462	26.824917259280163	25.785215151293944
43	23.788978387720853	22.808074579051027	26.52972727975969	26.87321975346843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	126.5
2	172.0
3	379.0
4	586.0
5	586.0
6	741.0
7	896.0
8	846.0
9	796.0
10	1049.0
11	1302.0
12	1302.0
13	2233.0
14	3164.0
15	4246.0
16	5328.0
17	5094.5
18	4861.0
19	4861.0
20	5882.0
21	6903.0
22	7452.0
23	8001.0
24	10369.5
25	12738.0
26	12738.0
27	16360.5
28	19983.0
29	23947.5
30	27912.0
31	34937.0
32	41962.0
33	41962.0
34	50537.5
35	59113.0
36	69501.5
37	79890.0
38	93036.5
39	106183.0
40	106183.0
41	120033.0
42	133883.0
43	150739.0
44	167595.0
45	188269.0
46	208943.0
47	208943.0
48	230203.0
49	251463.0
50	266042.5
51	280622.0
52	281477.5
53	282333.0
54	282333.0
55	266685.5
56	251038.0
57	232890.5
58	214743.0
59	200185.5
60	185628.0
61	185628.0
62	162369.5
63	139111.0
64	116732.0
65	94353.0
66	78522.0
67	62691.0
68	62691.0
69	51412.5
70	40134.0
71	32734.0
72	25334.0
73	20331.5
74	15329.0
75	15329.0
76	12211.0
77	9093.0
78	7358.0
79	5623.0
80	4379.0
81	3135.0
82	3135.0
83	2309.0
84	1483.0
85	1099.0
86	715.0
87	470.5
88	226.0
89	226.0
90	159.0
91	92.0
92	60.0
93	28.0
94	19.5
95	11.0
96	11.0
97	6.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2753481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.082760837400414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15543725153674	48.76042727038738
2	9.753095455837439	11.719858033948352
3	3.4172531479759316	6.159540108320749
4	1.6113769563892	3.87263905158522
5	0.9769191738674705	2.9348000540475017
6	0.636460049311026	2.294416615518867
7	0.44070694525836596	1.8535222993937803
8	0.3241332099482697	1.557985450622463
9	0.24903283790714206	1.346632239657053
>10	1.360676311983254	14.314808250700858
>50	0.052449725740460175	2.1226277619760268
>100	0.020826809353653362	2.1726979276365546
>500	0.0012089814008280745	0.5020803557727047
>1k	4.2314349024037635E-4	0.3879645804324751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.631766480320729E-5
5	0.0	3.631766480320729E-5	0.0	0.0	3.631766480320729E-5
6	0.0	3.631766480320729E-5	0.0	0.0	3.631766480320729E-5
7	0.0	3.631766480320729E-5	0.0	0.0	7.263532960641458E-5
8	0.0	3.631766480320729E-5	0.0	0.0	7.263532960641458E-5
9	0.0	7.263532960641458E-5	0.0	7.263532960641458E-5	1.8158832401603644E-4
10	0.0	7.263532960641458E-5	0.0	7.263532960641458E-5	1.8158832401603644E-4
11	0.0	7.263532960641458E-5	0.0	7.263532960641458E-5	1.8158832401603644E-4
12	0.0	7.263532960641458E-5	0.0	1.0895299440962186E-4	1.8158832401603644E-4
13	0.0	7.263532960641458E-5	0.0	1.0895299440962186E-4	1.8158832401603644E-4
14	0.0	7.263532960641458E-5	0.0	1.0895299440962186E-4	2.1790598881924373E-4
15	0.0	7.263532960641458E-5	0.0	1.8158832401603644E-4	2.54223653622451E-4
16	3.631766480320729E-5	7.263532960641458E-5	0.0	2.1790598881924373E-4	2.54223653622451E-4
17	3.631766480320729E-5	7.263532960641458E-5	0.0	4.7212964244169474E-4	2.54223653622451E-4
18	3.631766480320729E-5	7.263532960641458E-5	0.0	5.810826368513166E-4	2.905413184256583E-4
19	7.263532960641458E-5	7.263532960641458E-5	0.0	7.62670960867353E-4	2.905413184256583E-4
20	7.263532960641458E-5	7.263532960641458E-5	0.0	0.0011621652737026332	3.268589832288656E-4
21	7.263532960641458E-5	7.263532960641458E-5	0.0	0.0017069302457507424	3.268589832288656E-4
22	7.263532960641458E-5	7.263532960641458E-5	0.0	0.002215377552995644	3.268589832288656E-4
23	7.263532960641458E-5	7.263532960641458E-5	0.0	0.0024696012066180956	3.631766480320729E-4
24	7.263532960641458E-5	7.263532960641458E-5	0.0	0.0035954488155175214	3.631766480320729E-4
25	7.263532960641458E-5	7.263532960641458E-5	0.0	0.004612343430007325	3.9949431283528016E-4
26	7.263532960641458E-5	7.263532960641458E-5	0.0	0.006900356312609384	3.9949431283528016E-4
27	7.263532960641458E-5	7.263532960641458E-5	0.0	0.017214573116720252	3.9949431283528016E-4
28	7.263532960641458E-5	7.263532960641458E-5	0.0	0.05171635467976717	3.9949431283528016E-4
29	7.263532960641458E-5	7.263532960641458E-5	0.0	0.0987114129351174	3.9949431283528016E-4
30	7.263532960641458E-5	7.263532960641458E-5	0.0	0.16339317394962957	3.9949431283528016E-4
31	7.263532960641458E-5	7.263532960641458E-5	0.0	0.4639218501961699	3.9949431283528016E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1500	0.0	19.609999	37
TATACCG	100	5.883785E-6	16.650002	5
CATATAC	225	0.0	15.622222	3
TCTTATA	2655	0.0	15.608286	37
CGTTACG	605	0.0	14.677686	24
TTAGACT	270	0.0	14.388889	4
TCTATAC	265	0.0	13.962264	3
GTCTTAG	345	0.0	13.942028	1
TACGGAT	1360	0.0	13.73897	27
TCGTTAC	730	0.0	13.684932	23
GGACCGT	190	7.1377144E-9	13.631579	6
GTTACGG	1095	0.0	13.178082	25
GTCTAGA	295	0.0	13.169492	1
TATACTA	240	8.185452E-11	13.104166	5
TTAGACG	85	0.0094090905	13.058823	4
ACGGATG	1520	0.0	12.657895	28
TACTTAC	745	0.0	12.416107	31
GTACTTA	760	0.0	12.414474	30
TTACGGA	1550	0.0	12.412903	26
CTCGTTA	865	0.0	12.404624	22
>>END_MODULE