FastQCFastQC Report
Thu 9 Feb 2017
SRR2727534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566095
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10200.021.76470637
TACTTAC5550.017.66666631
GTACTTA5700.017.20175630
TCTTATA17350.015.88760937
CCCTATA700.002593056315.8571432
TCGTTAC7600.015.57894823
GTATATG1557.214112E-915.51612951
CTCGTTA8600.015.27325522
TGTTATA1101.4524576E-515.1363642
CCTATAC1101.4524576E-515.1363643
CGTTACG5900.015.05084724
TACGGAT12600.014.82936627
ACGATAC750.004105288514.83
AGTGTAC7650.014.75163427
TACATAT3400.014.69117635
TCTCGTT9400.014.5638321
ACGGATG13050.014.4597728
CTATACT1304.446152E-614.2307694
ATAGCAC1051.6562354E-414.0952393
TTACATA3550.014.07042234
Produced by FastQC (version 0.11.5)