##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1566095
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08959418170673	33.0	31.0	34.0	30.0	34.0
2	32.283035831159665	34.0	31.0	34.0	30.0	34.0
3	32.372622989026844	34.0	31.0	34.0	30.0	34.0
4	35.976532713532706	37.0	35.0	37.0	35.0	37.0
5	35.69669272936827	37.0	35.0	37.0	33.0	37.0
6	35.70038854603329	37.0	35.0	37.0	33.0	37.0
7	35.67480197561451	37.0	35.0	37.0	33.0	37.0
8	35.64926584913431	37.0	35.0	37.0	33.0	37.0
9	37.311115864618685	39.0	37.0	39.0	34.0	39.0
10	37.20138433492221	39.0	37.0	39.0	34.0	39.0
11	37.30822459684757	39.0	37.0	39.0	34.0	39.0
12	37.26748185774171	39.0	37.0	39.0	34.0	39.0
13	37.32422809599673	39.0	37.0	39.0	34.0	39.0
14	38.531451157177564	40.0	38.0	41.0	35.0	41.0
15	38.5196536608571	40.0	38.0	41.0	34.0	41.0
16	38.493459847582685	40.0	38.0	41.0	34.0	41.0
17	38.50808220446397	40.0	38.0	41.0	34.0	41.0
18	38.461043550997864	40.0	38.0	41.0	34.0	41.0
19	38.51698587888985	40.0	38.0	41.0	34.0	41.0
20	38.48992302510384	40.0	38.0	41.0	34.0	41.0
21	38.4592690737152	40.0	38.0	41.0	34.0	41.0
22	38.43864133401869	40.0	38.0	41.0	34.0	41.0
23	38.38704676280813	40.0	38.0	41.0	34.0	41.0
24	38.334810468075055	40.0	38.0	41.0	34.0	41.0
25	38.25554707728458	40.0	38.0	41.0	34.0	41.0
26	38.12977245952512	40.0	38.0	41.0	34.0	41.0
27	38.06324392836961	40.0	38.0	41.0	34.0	41.0
28	37.964813756509024	40.0	37.0	41.0	33.0	41.0
29	37.86695251565199	40.0	37.0	41.0	33.0	41.0
30	37.805255109045106	40.0	37.0	41.0	33.0	41.0
31	37.719686225931376	40.0	37.0	41.0	33.0	41.0
32	37.612761039400546	39.0	37.0	41.0	33.0	41.0
33	37.561766687206074	39.0	37.0	41.0	33.0	41.0
34	37.50710078251958	39.0	37.0	40.0	32.0	41.0
35	37.49469732040521	39.0	37.0	40.0	32.0	41.0
36	37.41303177648866	39.0	37.0	40.0	32.0	41.0
37	37.40284848620294	39.0	37.0	40.0	32.0	41.0
38	37.3687732864226	39.0	37.0	40.0	32.0	41.0
39	37.29323316912448	39.0	36.0	40.0	32.0	41.0
40	37.24889805535424	39.0	36.0	40.0	32.0	41.0
41	37.19230570303845	39.0	36.0	40.0	31.0	41.0
42	37.157969982663886	39.0	36.0	40.0	31.0	41.0
43	36.01174832944361	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	1.0
13	0.0
14	2.0
15	4.0
16	9.0
17	25.0
18	36.0
19	77.0
20	157.0
21	317.0
22	663.0
23	1164.0
24	1979.0
25	3240.0
26	4849.0
27	7176.0
28	10331.0
29	14741.0
30	19963.0
31	27029.0
32	36010.0
33	48464.0
34	67916.0
35	96041.0
36	147838.0
37	253765.0
38	483015.0
39	341278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2707466660707	16.78467781328719	12.099712980374754	27.84486254026735
2	18.916413116701094	19.907540730287753	34.66833110379638	26.507715049214763
3	20.4786427387866	21.54601093803377	29.412838940166463	28.562507383013163
4	16.018823889993904	14.475622487780115	32.75369629556317	36.751857326662815
5	16.526392077108987	35.232856244352995	32.73977632263688	15.500975355901142
6	37.58545937506984	32.34280168189031	14.490436403921855	15.581302539117997
7	30.793214970994736	30.039620840370475	19.918268048873152	19.248896139761637
8	28.44814650452239	34.291278626136986	18.93046079580102	18.3301140735396
9	29.499232166631018	13.56482205741031	16.944629795765902	39.99131598019277
10	17.01978487895051	26.12146772705359	32.26905136661569	24.589696027380203
11	38.37091619601621	20.425069998946423	19.745673155204504	21.458340649832863
12	22.702198781044572	23.028488054683784	28.904951487617293	25.36436167665435
13	32.55715649433783	18.465227205246173	22.77773698275009	26.199879317665914
14	23.745813631995503	18.844195275510106	23.945609940648556	33.464381151845835
15	27.66415830457284	25.764082000134092	20.8753619671859	25.696397728107172
16	25.747416344474633	24.800475066965923	23.485740009386404	25.96636857917304
17	25.878379025538038	24.08972635759644	23.556680788841035	26.47521382802448
18	25.643718931482447	24.988394701470856	24.274325631586848	25.093560735459853
19	27.48377333431241	24.11437364910813	23.952442220938067	24.449410795641388
20	26.008703175733274	23.355352006104354	23.974343829716588	26.661600988445784
21	25.586378859520014	24.60412682500104	24.97243142976639	24.83706288571255
22	26.76919343973386	23.15236304311041	23.14227425539319	26.936169261762537
23	25.716447597367974	23.894974442802	24.394752553325308	25.993825406504712
24	25.22541735973871	23.39972990144276	24.5149240627165	26.859928676102022
25	26.279440263840954	22.856148573362407	23.80979442498699	27.05461673780965
26	25.62009328935984	23.542696962827925	24.598571606447887	26.23863814136435
27	26.9721824027278	23.98641206312516	23.557830144403756	25.48357538974328
28	25.64869947225424	25.110992628161128	22.69868686126959	26.541621038315043
29	25.894150737982052	24.709803683684576	24.134551224542573	25.2614943537908
30	27.38045903984113	23.622641027523873	23.127843457772357	25.869056474862635
31	26.105121336828223	23.845296741257716	24.367614991427722	25.68196693048634
32	25.698696439232616	23.917642288622336	23.94956883203126	26.434092440113787
33	25.591806371899533	23.28990259211606	24.05920458209751	27.0590864538869
34	26.127469917214473	23.79434197797707	25.049949077163262	25.028239027645192
35	26.18059568544692	23.272726111762058	24.203257145958577	26.34342105683244
36	25.54678994569295	23.17643565684074	25.085323687260352	26.19145071020596
37	24.879716747706876	23.195974701407003	25.721236578879314	26.203071972006807
38	26.146498137086194	22.197376276662656	24.996440190409906	26.659685395841247
39	24.197638074318608	23.220366580571422	26.508928257864305	26.073067087245665
40	26.165334797697458	21.76668720607626	26.290550700947264	25.777427295279022
41	24.70654717625687	22.600863932264645	26.400250304100325	26.29233858737816
42	24.342712287568762	22.57168307158889	26.972884786682798	26.11271985415955
43	23.736427228233282	22.178475762964574	26.35382910998375	27.731267898818402
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	40.0
2	56.0
3	127.5
4	199.0
5	199.0
6	247.0
7	295.0
8	295.0
9	295.0
10	395.5
11	496.0
12	496.0
13	806.5
14	1117.0
15	1581.5
16	2046.0
17	1981.5
18	1917.0
19	1917.0
20	2260.5
21	2604.0
22	2853.5
23	3103.0
24	4095.0
25	5087.0
26	5087.0
27	6596.0
28	8105.0
29	10028.0
30	11951.0
31	15319.0
32	18687.0
33	18687.0
34	23364.5
35	28042.0
36	33647.0
37	39252.0
38	46493.5
39	53735.0
40	53735.0
41	61741.0
42	69747.0
43	79642.0
44	89537.0
45	103277.5
46	117018.0
47	117018.0
48	133269.5
49	149521.0
50	160313.0
51	171105.0
52	172459.5
53	173814.0
54	173814.0
55	161830.0
56	149846.0
57	137685.0
58	125524.0
59	117566.0
60	109608.0
61	109608.0
62	95845.5
63	82083.0
64	68667.5
65	55252.0
66	46023.5
67	36795.0
68	36795.0
69	29939.5
70	23084.0
71	18914.5
72	14745.0
73	11956.0
74	9167.0
75	9167.0
76	7439.0
77	5711.0
78	4516.0
79	3321.0
80	2568.5
81	1816.0
82	1816.0
83	1312.0
84	808.0
85	591.0
86	374.0
87	255.0
88	136.0
89	136.0
90	90.5
91	45.0
92	30.5
93	16.0
94	12.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1566095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.76486531791149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.44350580092639	55.53415785970203
2	8.45050208663221	11.114922631921946
3	2.7247026678723167	5.375691119519312
4	1.3050412889061362	3.4330345839690266
5	0.7852086129137512	2.581956933736848
6	0.4889914335398622	1.9295073461016905
7	0.33115161346009075	1.5244698881308372
8	0.25899076735155596	1.3625994346766102
9	0.20625563648765766	1.2207936739203826
>10	0.9431653149450094	10.35122071341375
>50	0.03728530549149053	1.6875001912058756
>100	0.02296182295511413	2.8127026625776463
>500	0.0019457813203917148	0.8479537539577685
>1k	2.9186719805875724E-4	0.2234892071662631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	6.385308681784949E-5
8	0.0	0.0	0.0	0.0	6.385308681784949E-5
9	0.0	0.0	0.0	1.2770617363569897E-4	6.385308681784949E-5
10	0.0	0.0	0.0	1.2770617363569897E-4	6.385308681784949E-5
11	0.0	0.0	0.0	2.5541234727139794E-4	6.385308681784949E-5
12	0.0	0.0	0.0	2.5541234727139794E-4	6.385308681784949E-5
13	0.0	0.0	0.0	2.5541234727139794E-4	6.385308681784949E-5
14	0.0	0.0	0.0	3.1926543408924746E-4	1.2770617363569897E-4
15	0.0	0.0	0.0	3.8311852090709697E-4	1.2770617363569897E-4
16	0.0	0.0	0.0	5.108246945427959E-4	1.2770617363569897E-4
17	0.0	0.0	0.0	5.746777813606455E-4	1.2770617363569897E-4
18	0.0	0.0	0.0	7.662370418141939E-4	1.2770617363569897E-4
19	0.0	0.0	0.0	9.577963022677424E-4	1.2770617363569897E-4
20	0.0	0.0	0.0	0.0013409148231748394	1.2770617363569897E-4
21	0.0	0.0	0.0	0.002234858038624732	1.9155926045354849E-4
22	0.0	0.0	0.0	0.0028733889068032273	1.9155926045354849E-4
23	0.0	0.0	0.0	0.00376733212225312	2.5541234727139794E-4
24	0.0	0.0	0.0	0.0050443938586101095	2.5541234727139794E-4
25	0.0	0.0	0.0	0.0061937494213314005	3.1926543408924746E-4
26	0.0	0.0	0.0	0.008428607459956133	3.1926543408924746E-4
27	0.0	0.0	0.0	0.02004986926080474	3.1926543408924746E-4
28	0.0	0.0	0.0	0.0568931003547039	3.8311852090709697E-4
29	0.0	0.0	0.0	0.11634032418212177	3.8311852090709697E-4
30	6.385308681784949E-5	0.0	0.0	0.19692291974624784	3.8311852090709697E-4
31	6.385308681784949E-5	0.0	0.0	0.543900593514442	3.8311852090709697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1020	0.0	21.764706	37
TACTTAC	555	0.0	17.666666	31
GTACTTA	570	0.0	17.201756	30
TCTTATA	1735	0.0	15.887609	37
CCCTATA	70	0.0025930563	15.857143	2
TCGTTAC	760	0.0	15.578948	23
GTATATG	155	7.214112E-9	15.5161295	1
CTCGTTA	860	0.0	15.273255	22
TGTTATA	110	1.4524576E-5	15.136364	2
CCTATAC	110	1.4524576E-5	15.136364	3
CGTTACG	590	0.0	15.050847	24
TACGGAT	1260	0.0	14.829366	27
ACGATAC	75	0.0041052885	14.8	3
AGTGTAC	765	0.0	14.751634	27
TACATAT	340	0.0	14.691176	35
TCTCGTT	940	0.0	14.56383	21
ACGGATG	1305	0.0	14.45977	28
CTATACT	130	4.446152E-6	14.230769	4
ATAGCAC	105	1.6562354E-4	14.095239	3
TTACATA	355	0.0	14.070422	34
>>END_MODULE