FastQCFastQC Report
Thu 9 Feb 2017
SRR2727533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209734
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1059.822543E-1021.1428593
CTTATAC7050.020.20567337
CGTCGAA555.143309E-420.18181822
GTATAAT656.902512E-519.9230771
TACTTAC3300.018.531
TAAGGGT1552.0008883E-1117.9032254
TAATACA1156.405753E-817.6956524
TCTTATA13350.017.32209837
TTTATAC752.0673785E-417.2666683
TAGTACT752.0673785E-417.2666684
GATCGAG904.4462668E-516.4444457
CCTAATA803.38275E-416.18752
GGTACCG957.0601636E-515.5789485
GTACTTA3850.015.37662330
TCGTTAT855.364441E-415.23529423
TTATACA3400.015.23529437
TATTATA855.364441E-415.2352941
GTGTACT4750.014.41052628
TAATACC908.274457E-414.3888884
TAGACAG1551.2100645E-714.322585
Produced by FastQC (version 0.11.5)