##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727533.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1209734
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57374844387279	34.0	31.0	34.0	31.0	34.0
2	32.75145445197043	34.0	31.0	34.0	31.0	34.0
3	32.85091267997758	34.0	31.0	34.0	31.0	34.0
4	36.30112900852584	37.0	37.0	37.0	35.0	37.0
5	36.15764787961651	37.0	35.0	37.0	35.0	37.0
6	36.180509103654195	37.0	36.0	37.0	35.0	37.0
7	36.15499440372842	37.0	35.0	37.0	35.0	37.0
8	36.144204428411534	37.0	35.0	37.0	35.0	37.0
9	37.86923488965343	39.0	38.0	39.0	35.0	39.0
10	37.83817847559877	39.0	37.0	39.0	35.0	39.0
11	37.9188656349247	39.0	38.0	39.0	35.0	39.0
12	37.879647922601166	39.0	38.0	39.0	35.0	39.0
13	37.91656016942567	39.0	38.0	39.0	35.0	39.0
14	39.25519990344985	40.0	39.0	41.0	36.0	41.0
15	39.27708074667654	40.0	39.0	41.0	36.0	41.0
16	39.258659341640396	40.0	39.0	41.0	36.0	41.0
17	39.27311375889245	40.0	39.0	41.0	36.0	41.0
18	39.23971798759066	40.0	39.0	41.0	36.0	41.0
19	39.27885303711395	40.0	39.0	41.0	36.0	41.0
20	39.25025501473878	40.0	39.0	41.0	36.0	41.0
21	39.229042913566126	40.0	39.0	41.0	36.0	41.0
22	39.19402199161138	40.0	39.0	41.0	36.0	41.0
23	39.15683613091804	40.0	39.0	41.0	36.0	41.0
24	39.11139721624754	40.0	39.0	41.0	36.0	41.0
25	39.04778323168564	40.0	39.0	41.0	36.0	41.0
26	38.948598617547326	40.0	38.0	41.0	35.0	41.0
27	38.90546682163186	40.0	38.0	41.0	35.0	41.0
28	38.81807240269348	40.0	38.0	41.0	35.0	41.0
29	38.744473578489156	40.0	38.0	41.0	35.0	41.0
30	38.687009706266004	40.0	38.0	41.0	35.0	41.0
31	38.62064801022373	40.0	38.0	41.0	35.0	41.0
32	38.531823524840995	40.0	38.0	41.0	35.0	41.0
33	38.48250855146669	40.0	38.0	41.0	34.0	41.0
34	38.437964048294916	40.0	38.0	41.0	34.0	41.0
35	38.42278219840064	40.0	38.0	41.0	34.0	41.0
36	38.36390727217719	40.0	38.0	41.0	34.0	41.0
37	38.34631993479558	40.0	38.0	41.0	34.0	41.0
38	38.30786354686237	40.0	38.0	41.0	34.0	41.0
39	38.262766029556914	40.0	38.0	41.0	34.0	41.0
40	38.21231030953912	40.0	38.0	41.0	34.0	41.0
41	38.15950365948217	40.0	38.0	41.0	34.0	41.0
42	38.1247059270881	40.0	38.0	41.0	34.0	41.0
43	37.00017689839254	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	3.0
11	1.0
12	0.0
13	0.0
14	2.0
15	4.0
16	2.0
17	4.0
18	12.0
19	25.0
20	73.0
21	119.0
22	269.0
23	516.0
24	807.0
25	1372.0
26	2084.0
27	3174.0
28	4552.0
29	6638.0
30	9264.0
31	12880.0
32	17412.0
33	24166.0
34	34954.0
35	50846.0
36	78594.0
37	133819.0
38	314132.0
39	514008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.136810240928995	15.88373973121364	11.478721768587144	28.50072825927022
2	19.27241856474233	19.158426563194883	33.87579418285342	27.693360689209364
3	20.742163153222112	20.719100231951817	28.65580367254289	29.88293294228318
4	16.01608287441702	13.941329250893173	31.996868733126455	38.04571914156335
5	17.09160856849522	34.2889428585127	32.6791674864061	15.940281086585978
6	38.8358928491718	31.261996438886563	14.144266425511724	15.75784428642991
7	31.726148062301302	28.65117455572878	19.60993077817107	20.01274660379885
8	28.93603056539702	33.30881003592525	18.639138852012096	19.11602054666563
9	29.15690556767025	13.21323530627394	16.648949273145995	40.98090985290981
10	17.332239980028668	25.459315849599996	32.075563718966315	25.132880451405022
11	39.06073566585712	19.731031780540185	19.281098158768785	21.927134394833907
12	23.29817959981285	22.555123688348015	28.586366920331248	25.560329791507886
13	33.50703543092944	17.79928480145222	22.04691279239899	26.646766975219343
14	24.161427222844033	18.22483289714929	23.332484661917412	34.28125521808927
15	28.084769048402375	25.12370488057705	20.39035027534979	26.401175795670785
16	26.632052996774497	23.950802407802048	22.748719966538097	26.668424628885358
17	26.566501396174697	23.458793420702403	23.215930113562155	26.75877506956075
18	26.37604630439419	23.883597551197205	23.69372109901846	26.046635045390143
19	27.794291968317005	23.404070646935608	23.333889929521696	25.46774745522569
20	26.846232312227315	22.714084253232528	23.52492366090397	26.914759773636188
21	26.45457596463355	23.762331223227587	23.968409584255713	25.814683227883155
22	27.36692529101439	22.814106241537395	22.770625608604867	27.04834285884335
23	26.587332421838187	23.199728204712773	23.3657151076187	26.847224265830338
24	26.097803318746106	22.904539345013035	23.654208280498025	27.34344905574283
25	26.993289433875546	22.527100999062604	22.95628625797076	27.52332330909109
26	26.49111292234491	23.02497904498014	23.59684029712317	26.887067735551785
27	27.561430860007242	23.2748686901418	22.89660371618885	26.26709673366211
28	26.45457596463355	24.167544270062674	22.281344493913537	27.096535271390238
29	26.563608198165877	23.740177592760062	23.543688116561164	26.1525260925129
30	27.52745644910369	23.050687175858496	22.859736107276476	26.56212026776134
31	26.621802809543254	23.011752996939823	23.66503710733103	26.701407086185892
32	26.171042559769337	23.260154711696952	23.45441229228905	27.114390436244662
33	26.302724400570703	22.778313249028297	23.400433483724523	27.51852886667648
34	26.720171541843087	23.203613356324613	24.237311673475325	25.83890342835698
35	26.69652998097102	22.928346231485598	23.677436527368826	26.69768726017455
36	26.22560000793563	22.656798932657924	24.205238506977565	26.91236255242888
37	25.506268320143104	22.366239189772298	24.902581889903068	27.22491060018153
38	26.121527542418416	21.66368805043092	24.67509386360969	27.539690543540978
39	24.97350657251925	22.028148336741797	26.032665032147563	26.96568005859139
40	26.122188844820432	21.082733890260172	26.230146461949488	26.56493080296991
41	25.18495801556375	21.4832351574809	26.399605202466	26.93220162448935
42	24.339565557386997	21.471331714244617	27.124805949076407	27.064296779291976
43	23.970558817062262	21.096538577902248	26.736621439093224	28.196281165942267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	8.0
2	11.0
3	34.5
4	58.0
5	58.0
6	81.0
7	104.0
8	97.5
9	91.0
10	127.0
11	163.0
12	163.0
13	266.5
14	370.0
15	483.0
16	596.0
17	598.0
18	600.0
19	600.0
20	802.5
21	1005.0
22	1198.0
23	1391.0
24	1914.5
25	2438.0
26	2438.0
27	3300.5
28	4163.0
29	5392.0
30	6621.0
31	8690.5
32	10760.0
33	10760.0
34	13697.0
35	16634.0
36	20580.5
37	24527.0
38	29710.5
39	34894.0
40	34894.0
41	40911.0
42	46928.0
43	54993.0
44	63058.0
45	73774.5
46	84491.0
47	84491.0
48	96427.5
49	108364.0
50	118988.5
51	129613.0
52	132705.0
53	135797.0
54	135797.0
55	129524.0
56	123251.0
57	114735.5
58	106220.0
59	99689.0
60	93158.0
61	93158.0
62	82729.5
63	72301.0
64	61058.5
65	49816.0
66	41972.0
67	34128.0
68	34128.0
69	28303.0
70	22478.0
71	18520.5
72	14563.0
73	11811.0
74	9059.0
75	9059.0
76	7405.0
77	5751.0
78	4529.0
79	3307.0
80	2513.5
81	1720.0
82	1720.0
83	1227.5
84	735.0
85	552.5
86	370.0
87	250.5
88	131.0
89	131.0
90	89.0
91	47.0
92	29.5
93	12.0
94	7.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1209734.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.12356168035728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98368535664223	62.73573196004576
2	7.345662109636257	10.595906284948281
3	2.2838683801279807	4.941621659519342
4	1.0854610876646509	3.131492788312366
5	0.6172165129060053	2.225792661935566
6	0.3908584055314869	1.6914060191781777
7	0.2772033027374776	1.3995022652089655
8	0.19359701059179243	1.117032474763994
9	0.14227294883201222	0.9235108620478754
>10	0.6369719323679448	7.665895601915035
>50	0.028632274703447865	1.4352515038045763
>100	0.013767575984829791	1.740996345327964
>500	8.031022738355386E-4	0.39585957299202706
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.266280025195622E-5
2	0.0	0.0	0.0	8.266280025195622E-5	8.266280025195622E-5
3	0.0	0.0	0.0	8.266280025195622E-5	8.266280025195622E-5
4	0.0	0.0	0.0	1.6532560050391243E-4	8.266280025195622E-5
5	0.0	0.0	0.0	1.6532560050391243E-4	8.266280025195622E-5
6	0.0	0.0	0.0	1.6532560050391243E-4	8.266280025195622E-5
7	0.0	0.0	0.0	1.6532560050391243E-4	8.266280025195622E-5
8	0.0	0.0	0.0	1.6532560050391243E-4	8.266280025195622E-5
9	0.0	0.0	0.0	2.4798840075586863E-4	8.266280025195622E-5
10	0.0	0.0	0.0	2.4798840075586863E-4	8.266280025195622E-5
11	0.0	0.0	0.0	2.4798840075586863E-4	8.266280025195622E-5
12	0.0	0.0	0.0	2.4798840075586863E-4	8.266280025195622E-5
13	8.266280025195622E-5	0.0	0.0	3.3065120100782486E-4	8.266280025195622E-5
14	8.266280025195622E-5	0.0	0.0	4.133140012597811E-4	2.4798840075586863E-4
15	8.266280025195622E-5	0.0	0.0	4.133140012597811E-4	2.4798840075586863E-4
16	8.266280025195622E-5	0.0	0.0	5.786396017636935E-4	2.4798840075586863E-4
17	8.266280025195622E-5	0.0	0.0	7.43965202267606E-4	2.4798840075586863E-4
18	8.266280025195622E-5	0.0	0.0	8.266280025195622E-4	3.3065120100782486E-4
19	8.266280025195622E-5	0.0	0.0	9.919536030234745E-4	3.3065120100782486E-4
20	8.266280025195622E-5	0.0	0.0	0.0013226048040312994	3.3065120100782486E-4
21	8.266280025195622E-5	0.0	0.0	0.001901244405794993	3.3065120100782486E-4
22	8.266280025195622E-5	0.0	0.0	0.003471837610582161	3.3065120100782486E-4
23	8.266280025195622E-5	0.0	0.0	0.004463791213605636	3.3065120100782486E-4
24	8.266280025195622E-5	0.0	0.0	0.006199710018896716	3.3065120100782486E-4
25	8.266280025195622E-5	0.0	0.0	0.007935628824187796	3.3065120100782486E-4
26	8.266280025195622E-5	0.0	0.0	0.010663501232502351	3.3065120100782486E-4
27	8.266280025195622E-5	0.0	0.0	0.02314558407054774	3.3065120100782486E-4
28	8.266280025195622E-5	0.0	0.0	0.062327751389974984	3.3065120100782486E-4
29	8.266280025195622E-5	0.0	0.0	0.11886910676231303	3.3065120100782486E-4
30	8.266280025195622E-5	0.0	0.0	0.19830805780444297	3.3065120100782486E-4
31	8.266280025195622E-5	0.0	0.0	0.5018458603296262	3.3065120100782486E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATAC	105	9.822543E-10	21.142859	3
CTTATAC	705	0.0	20.205673	37
CGTCGAA	55	5.143309E-4	20.181818	22
GTATAAT	65	6.902512E-5	19.923077	1
TACTTAC	330	0.0	18.5	31
TAAGGGT	155	2.0008883E-11	17.903225	4
TAATACA	115	6.405753E-8	17.695652	4
TCTTATA	1335	0.0	17.322098	37
TTTATAC	75	2.0673785E-4	17.266668	3
TAGTACT	75	2.0673785E-4	17.266668	4
GATCGAG	90	4.4462668E-5	16.444445	7
CCTAATA	80	3.38275E-4	16.1875	2
GGTACCG	95	7.0601636E-5	15.578948	5
GTACTTA	385	0.0	15.376623	30
TCGTTAT	85	5.364441E-4	15.235294	23
TTATACA	340	0.0	15.235294	37
TATTATA	85	5.364441E-4	15.235294	1
GTGTACT	475	0.0	14.410526	28
TAATACC	90	8.274457E-4	14.388888	4
TAGACAG	155	1.2100645E-7	14.32258	5
>>END_MODULE