FastQCFastQC Report
Thu 9 Feb 2017
SRR2727532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340962
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7400.019.2537
GTACTAG801.6169985E-518.51
TAGATCG500.007035478518.55
TAGTACC1054.7987305E-717.619054
TATAGTA1059.34534E-615.8571442
TCTTATA12500.015.5437
TTAGAGT1201.9359886E-615.4166664
CTATACC1201.9359886E-615.4166664
ATAGTAC1455.3469194E-815.3103453
CTTACAC2301.8189894E-1214.4782613
TACTTAC2700.014.38888931
GTTCTAG1803.3305696E-914.3888881
TGTTACG2600.014.23076924
ATAGGAC1458.912375E-714.0344833
GTGTTAG1203.301166E-513.8749991
TCTATAC1356.568449E-613.7037043
GTTACGG5000.013.6925
GTTTAAG1102.456838E-413.4545451
TTACACA2202.8376235E-1013.4545454
TAGAGTG1409.560879E-613.2142855
Produced by FastQC (version 0.11.5)