##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340962
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.579892644236004	34.0	31.0	34.0	31.0	34.0
2	32.75242102311624	34.0	31.0	34.0	31.0	34.0
3	32.844298347007594	34.0	31.0	34.0	31.0	34.0
4	36.30428453602712	37.0	37.0	37.0	35.0	37.0
5	36.15979200007159	37.0	35.0	37.0	35.0	37.0
6	36.182163998681546	37.0	36.0	37.0	35.0	37.0
7	36.15640562521533	37.0	35.0	37.0	35.0	37.0
8	36.143102489108564	37.0	35.0	37.0	35.0	37.0
9	37.86997170687909	39.0	38.0	39.0	35.0	39.0
10	37.83436741682464	39.0	37.0	39.0	35.0	39.0
11	37.92064428373064	39.0	38.0	39.0	35.0	39.0
12	37.88436957945117	39.0	38.0	39.0	35.0	39.0
13	37.918026014159985	39.0	38.0	39.0	35.0	39.0
14	39.26794122428525	40.0	39.0	41.0	36.0	41.0
15	39.287433946674106	40.0	39.0	41.0	36.0	41.0
16	39.26609329720007	40.0	39.0	41.0	36.0	41.0
17	39.27430009202349	40.0	39.0	41.0	36.0	41.0
18	39.24883628320564	40.0	39.0	41.0	36.0	41.0
19	39.28922296082961	40.0	39.0	41.0	36.0	41.0
20	39.259612129202765	40.0	39.0	41.0	36.0	41.0
21	39.24098222022697	40.0	39.0	41.0	36.0	41.0
22	39.21534987568626	40.0	39.0	41.0	36.0	41.0
23	39.17457765395291	40.0	39.0	41.0	36.0	41.0
24	39.13536998065568	40.0	39.0	41.0	36.0	41.0
25	39.07053592868404	40.0	39.0	41.0	36.0	41.0
26	38.975730855907926	40.0	38.0	41.0	36.0	41.0
27	38.93118969814208	40.0	38.0	41.0	35.0	41.0
28	38.85441123611258	40.0	38.0	41.0	35.0	41.0
29	38.77365801566338	40.0	38.0	41.0	35.0	41.0
30	38.7188980746658	40.0	38.0	41.0	35.0	41.0
31	38.660461668563315	40.0	38.0	41.0	35.0	41.0
32	38.57157324368625	40.0	38.0	41.0	35.0	41.0
33	38.52660478074696	40.0	38.0	41.0	35.0	41.0
34	38.48421804644725	40.0	38.0	41.0	34.0	41.0
35	38.46862923781584	40.0	38.0	41.0	34.0	41.0
36	38.40221199407589	40.0	38.0	41.0	34.0	41.0
37	38.392026023108784	40.0	38.0	41.0	34.0	41.0
38	38.35516368099916	40.0	38.0	41.0	34.0	41.0
39	38.313797109836074	40.0	38.0	41.0	34.0	41.0
40	38.25992086278358	40.0	38.0	41.0	34.0	41.0
41	38.21151829805766	40.0	38.0	41.0	34.0	41.0
42	38.171512690143345	40.0	38.0	41.0	34.0	41.0
43	37.04897827082348	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	2.0
16	1.0
17	6.0
18	10.0
19	27.0
20	69.0
21	143.0
22	251.0
23	470.0
24	781.0
25	1342.0
26	2245.0
27	3274.0
28	4859.0
29	7058.0
30	10029.0
31	13865.0
32	19070.0
33	26158.0
34	38129.0
35	56342.0
36	85477.0
37	148950.0
38	351880.0
39	570521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96038068192835	16.197550713592182	11.567963894577177	28.274104709902293
2	19.062285135596685	19.113666159070878	34.061069590338874	27.762979114993563
3	20.585370800962295	20.688729434540278	28.721097242129158	30.00480252236827
4	15.93594747651313	13.905614029331181	32.041250982503605	38.117187511652084
5	16.951188773432804	34.25719744481947	32.73634897931485	16.05526480243288
6	38.7761920173726	31.500519776101036	14.058638499823262	15.664649706703099
7	31.65652717974111	28.67821757812675	19.74299047996886	19.922264762163284
8	28.813195303073464	33.44330413538937	18.60582179062494	19.137678770912224
9	28.814612196318762	13.38665823490897	16.808007982329105	40.990721586443165
10	17.401462532122462	25.420183420559272	31.983158359446428	25.19519568787184
11	38.99484101712055	19.733370520566577	19.481909256190704	21.789879206122173
12	23.251069008666914	22.553510092008572	28.588953303673037	25.606467595651477
13	33.19266317762919	17.83175063872056	22.365137863712768	26.61044831993748
14	24.219403681834383	18.172625324207548	23.276871380397058	34.33109961356101
15	27.786618860191414	25.169393316141697	20.39707314599519	26.646914677671703
16	26.696729661243197	24.022157227423296	22.67961359084001	26.601499520493498
17	26.40097183961962	23.663310369719646	23.243760822454327	26.69195696820641
18	26.39918207973082	23.82051094661892	23.790532468481583	25.98977450516868
19	27.739786809767914	23.508943579310973	23.353980202272698	25.397289408648415
20	26.68621482189652	22.90750968334673	23.39514467971501	27.01113081504174
21	26.28329512693126	23.784268308870796	23.94892621864005	25.983510345557892
22	27.238206600932763	22.860826779580627	22.753217466266754	27.147749153219852
23	26.502988153281002	23.126382403080772	23.555328189762275	26.81530125387595
24	26.129375776494783	22.982828745333574	23.574791828552936	27.313003649618707
25	26.95743801837785	22.475357243531136	23.16530968066209	27.40189505742892
26	26.456528969500997	22.99975689094844	23.694258301130084	26.84945583842048
27	27.259310852954822	23.401035972682298	23.020861142970496	26.31879203139239
28	26.514696165886882	23.944302672260662	22.57953618372482	26.96146497812764
29	26.46778954213468	23.89113188889767	23.556073923049272	26.08500464591838
30	27.37825531223107	23.083428165749666	22.974998545820092	26.563317976199173
31	26.586584854753525	23.112437190613903	23.62460681212443	26.67637114250814
32	26.13355188290198	23.245699729000524	23.513045112389463	27.107703275708033
33	26.247798222470138	22.919292269281307	23.51476029894956	27.318149209298998
34	26.736178952125417	23.196555905387324	24.190469230298845	25.876795912188417
35	26.61715991952046	22.967392066292707	23.72475879256832	26.69068922161851
36	26.130718096411382	22.78849065074178	24.226189854746067	26.85460139810077
37	25.51011885497128	22.37252062325405	24.925911397936705	27.191449123837963
38	26.109166404417127	21.829925083633988	24.724787130433228	27.33612138151566
39	24.994369713683163	22.09659930706463	25.926760042417307	26.9822709368349
40	26.172330013825896	21.221183001457163	26.114759404069616	26.49172758064733
41	25.236061871999354	21.50911062356726	26.286278060079255	26.968549444354128
42	24.471461532839854	21.612096390501744	26.93879468620289	26.977647390455513
43	23.993968509174756	21.143030152979726	26.713434086871963	28.149567250973558
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.5
2	19.0
3	46.5
4	74.0
5	74.0
6	90.5
7	107.0
8	104.0
9	101.0
10	146.0
11	191.0
12	191.0
13	326.5
14	462.0
15	600.5
16	739.0
17	740.0
18	741.0
19	741.0
20	937.5
21	1134.0
22	1316.5
23	1499.0
24	2111.5
25	2724.0
26	2724.0
27	3619.0
28	4514.0
29	5864.5
30	7215.0
31	9694.0
32	12173.0
33	12173.0
34	15361.5
35	18550.0
36	22958.0
37	27366.0
38	33435.0
39	39504.0
40	39504.0
41	46418.0
42	53332.0
43	62117.0
44	70902.0
45	82659.0
46	94416.0
47	94416.0
48	107277.0
49	120138.0
50	130778.0
51	141418.0
52	144963.5
53	148509.0
54	148509.0
55	142886.5
56	137264.0
57	128063.0
58	118862.0
59	112166.5
60	105471.0
61	105471.0
62	93107.0
63	80743.0
64	68194.5
65	55646.0
66	46460.0
67	37274.0
68	37274.0
69	30592.5
70	23911.0
71	19511.5
72	15112.0
73	12034.0
74	8956.0
75	8956.0
76	7263.5
77	5571.0
78	4386.5
79	3202.0
80	2496.5
81	1791.0
82	1791.0
83	1277.0
84	763.0
85	565.5
86	368.0
87	250.5
88	133.0
89	133.0
90	88.0
91	43.0
92	25.5
93	8.0
94	7.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1340962.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.12764444868506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26090789464031	57.40497173969455
2	8.735390200124195	11.90243115349178
3	2.8009823933432343	5.724729978021443
4	1.311869160274816	3.5749822285759083
5	0.7626803293675248	2.597980715357837
6	0.4800658108539783	1.9623451724297718
7	0.34821462590431373	1.6606129557808675
8	0.23016279204852255	1.2544359089598707
9	0.18919996280978124	1.1600773016408292
>10	0.8369869463593733	9.385481447152879
>50	0.029071115479459574	1.323685796839116
>100	0.01359189374341557	1.6327778545333331
>500	7.67265669708161E-4	0.3393946463849705
>1k	1.0960938138688014E-4	0.07609310113669636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.457332869984384E-5
2	0.0	0.0	0.0	7.457332869984384E-5	7.457332869984384E-5
3	0.0	0.0	0.0	7.457332869984384E-5	7.457332869984384E-5
4	0.0	0.0	0.0	1.4914665739968768E-4	2.2371998609953153E-4
5	0.0	0.0	0.0	2.9829331479937535E-4	2.2371998609953153E-4
6	0.0	0.0	0.0	2.9829331479937535E-4	2.2371998609953153E-4
7	0.0	0.0	0.0	2.9829331479937535E-4	2.2371998609953153E-4
8	0.0	0.0	0.0	2.9829331479937535E-4	2.9829331479937535E-4
9	0.0	0.0	0.0	4.4743997219906306E-4	3.728666434992192E-4
10	0.0	0.0	0.0	4.4743997219906306E-4	3.728666434992192E-4
11	0.0	0.0	0.0	5.220133008989069E-4	3.728666434992192E-4
12	0.0	0.0	0.0	5.220133008989069E-4	3.728666434992192E-4
13	0.0	0.0	0.0	5.220133008989069E-4	3.728666434992192E-4
14	0.0	0.0	0.0	5.220133008989069E-4	3.728666434992192E-4
15	0.0	0.0	0.0	5.965866295987507E-4	5.220133008989069E-4
16	0.0	0.0	0.0	5.965866295987507E-4	5.220133008989069E-4
17	0.0	0.0	0.0	7.457332869984384E-4	5.220133008989069E-4
18	0.0	0.0	0.0	9.694532730979699E-4	5.965866295987507E-4
19	0.0	0.0	0.0	0.0011185999304976577	5.965866295987507E-4
20	0.0	0.0	0.0	0.0011931732591975014	5.965866295987507E-4
21	0.0	0.0	0.0	0.0014914665739968768	5.965866295987507E-4
22	0.0	0.0	0.0	0.0023117731896951594	5.965866295987507E-4
23	7.457332869984384E-5	0.0	0.0	0.0029829331479937536	5.965866295987507E-4
24	7.457332869984384E-5	0.0	0.0	0.004101533078491411	6.711599582985946E-4
25	7.457332869984384E-5	0.0	0.0	0.005369279666388757	6.711599582985946E-4
26	7.457332869984384E-5	0.0	0.0	0.0075319061986842285	7.457332869984384E-4
27	7.457332869984384E-5	0.0	0.0	0.020731385378556587	7.457332869984384E-4
28	7.457332869984384E-5	0.0	0.0	0.06032982291817367	7.457332869984384E-4
29	7.457332869984384E-5	0.0	0.0	0.11998848587804875	7.457332869984384E-4
30	7.457332869984384E-5	0.0	0.0	0.19582956116578992	7.457332869984384E-4
31	7.457332869984384E-5	0.0	0.0	0.49658379581226014	7.457332869984384E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	740	0.0	19.25	37
GTACTAG	80	1.6169985E-5	18.5	1
TAGATCG	50	0.0070354785	18.5	5
TAGTACC	105	4.7987305E-7	17.61905	4
TATAGTA	105	9.34534E-6	15.857144	2
TCTTATA	1250	0.0	15.54	37
TTAGAGT	120	1.9359886E-6	15.416666	4
CTATACC	120	1.9359886E-6	15.416666	4
ATAGTAC	145	5.3469194E-8	15.310345	3
CTTACAC	230	1.8189894E-12	14.478261	3
TACTTAC	270	0.0	14.388889	31
GTTCTAG	180	3.3305696E-9	14.388888	1
TGTTACG	260	0.0	14.230769	24
ATAGGAC	145	8.912375E-7	14.034483	3
GTGTTAG	120	3.301166E-5	13.874999	1
TCTATAC	135	6.568449E-6	13.703704	3
GTTACGG	500	0.0	13.69	25
GTTTAAG	110	2.456838E-4	13.454545	1
TTACACA	220	2.8376235E-10	13.454545	4
TAGAGTG	140	9.560879E-6	13.214285	5
>>END_MODULE