FastQCFastQC Report
Thu 9 Feb 2017
SRR2727531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580860
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.0054969629.64
CATATAA2250.018.91111237
TACTTAC6850.018.63503631
CTTATAC11100.018.537
GTACTTA7700.017.5389630
ACTATAC752.0678845E-417.2666663
ACTTACA5800.017.22413832
CTTACAT4600.016.89130433
ACATATA2650.016.75471736
TACATAT3550.016.67605835
TTACATA3900.016.60256434
TCGTTAC7100.015.89436723
TCTTATA18050.015.78393437
AGTGTAC8600.015.70348827
GTTACGG9700.015.0670125
CGTTACG6150.015.0406524
CTCGTTA8250.015.02424322
TATACCA1001.0937762E-414.85
GAATAAG1252.9602616E-614.7999991
GTGTACT9400.014.76063928
Produced by FastQC (version 0.11.5)