FastQCFastQC Report
Thu 9 Feb 2017
SRR2727530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916298
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT29050.3170365972642088No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG26470.2888798185743066No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG17730.19349600239223483No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT15320.16719451532143473No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG14110.15398920438547284No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG13640.14885986873266122No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG13520.14755025111917738No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT11380.12419540367871588No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC9300.10149536504499629No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA9220.10062228663600707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC2350.023.6170231
GTACTTA2650.021.6415130
ACTTACA2500.019.24000232
TATAGTA801.6160871E-518.55
TATACCG500.007033820318.4999985
CTTATAC4900.018.1224537
ACATATA1156.40066E-817.69565236
ATAGGAC852.7221402E-517.4117643
TTAGACT852.7221402E-517.4117644
TACCGTC650.001579603317.0769237
CAATACT1201.0404983E-716.9583324
TACATAT1655.4569682E-1116.81818235
AGTGTAC3200.016.76562527
TCTTATA9300.016.51075437
CGTTACG2650.016.05660424
TAATCCC1504.665708E-916.0333335
ATAGTAT700.00259195515.8571436
TTACATA1659.731593E-1015.6969734
CGGCTAC957.0571754E-515.57894825
TAGGCAG1201.9343606E-615.4166665
Produced by FastQC (version 0.11.5)