FastQCFastQC Report
Thu 9 Feb 2017
SRR2727529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686288
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT30330.1798625145882554No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG25000.14825462791646504No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG19620.11635023198884177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.11557930792367614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC8650.021.60115837
TACTTAC3800.019.47368431
TATACCG1005.8810674E-616.655
TCTTATA16600.016.60542137
TATACTG2003.6379788E-1215.7249995
TTTATAC1902.7284841E-1115.5789483
GTACTTA5050.015.38613930
TCGTTAC3900.015.17948723
TAATACT1601.0973963E-815.031254
CGTATAC750.004105462714.83
GTTCTAG2059.276846E-1114.4390241
GTATTAG2059.276846E-1114.4390241
CTATACC1803.3323886E-914.3888884
ACGGATG8650.014.3294828
CGACTTA1956.730261E-1014.2307719
TACGGAT8600.014.19767427
GTCTTAG2353.6379788E-1214.1702141
CGTTACG3400.014.14705924
CTCGTTA4450.014.13483222
CATATAG1051.6563546E-414.0952383
Produced by FastQC (version 0.11.5)