FastQCFastQC Report
Thu 9 Feb 2017
SRR2727528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532697
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG603.7273305E-521.5833345
CTTATAC9000.017.88333337
TACTTAC4450.017.04494331
GTACTTA4800.016.187530
ATACCGT803.3834856E-416.18756
TCTTATA15450.015.80582637
GTACTAG1152.2106837E-514.4782611
GTCTTAT1458.914758E-714.0344841
CTCGTTA4900.013.96938922
GTATTAG1203.3017703E-513.8750011
CTATACC1203.3017703E-513.8750014
AATAGGT800.00629988413.8750017
TTTTACG800.00629988413.8750012
CTCTTAT24200.013.530992537
GTGTACT6150.013.23577228
CGTTACA1409.563008E-613.21428524
TATAGTA1551.8884857E-613.1290325
GTACTAC1000.001832964812.9500011
TCTATAC1000.001832964812.9500013
AGTGTAC6300.012.92063527
Produced by FastQC (version 0.11.5)