##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727528.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1532697
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8038066232269	31.0	31.0	34.0	30.0	34.0
2	31.999461080696314	33.0	31.0	34.0	30.0	34.0
3	32.095958300955765	33.0	31.0	34.0	30.0	34.0
4	35.79613061159512	37.0	35.0	37.0	35.0	37.0
5	35.35885566423109	37.0	35.0	37.0	33.0	37.0
6	35.33968227249091	37.0	35.0	37.0	32.0	37.0
7	35.311879647445	37.0	35.0	37.0	32.0	37.0
8	35.26416571572855	37.0	35.0	37.0	32.0	37.0
9	36.905261770591316	39.0	37.0	39.0	33.0	39.0
10	36.75449093982699	38.0	37.0	39.0	33.0	39.0
11	36.86829882227211	39.0	37.0	39.0	33.0	39.0
12	36.82254418192245	38.0	37.0	39.0	33.0	39.0
13	36.89611188643287	39.0	37.0	39.0	33.0	39.0
14	38.01292427661827	39.0	37.0	40.0	33.0	41.0
15	37.993726744425025	39.0	37.0	40.0	33.0	41.0
16	37.96202315265183	39.0	37.0	40.0	33.0	41.0
17	37.96664441830316	39.0	37.0	40.0	33.0	41.0
18	37.92416178801159	39.0	37.0	40.0	33.0	41.0
19	37.97837276382742	40.0	37.0	40.0	33.0	41.0
20	37.947846834697266	39.0	37.0	40.0	33.0	41.0
21	37.913377529935794	39.0	37.0	40.0	33.0	41.0
22	37.89775669946506	39.0	37.0	40.0	33.0	41.0
23	37.83357310675235	39.0	37.0	40.0	33.0	41.0
24	37.76785561660263	39.0	37.0	40.0	33.0	41.0
25	37.681820346748246	39.0	37.0	40.0	33.0	41.0
26	37.53081528834466	39.0	37.0	40.0	32.0	41.0
27	37.45236207808849	39.0	37.0	40.0	32.0	41.0
28	37.34857313611236	39.0	36.0	40.0	32.0	41.0
29	37.22374807284153	39.0	36.0	40.0	31.0	41.0
30	37.15026779591791	39.0	36.0	40.0	31.0	41.0
31	37.06660481491123	39.0	36.0	40.0	31.0	41.0
32	36.94040244092603	39.0	36.0	40.0	31.0	41.0
33	36.893952946994744	39.0	36.0	40.0	31.0	41.0
34	36.82827656085971	39.0	36.0	40.0	31.0	41.0
35	36.80820344790914	39.0	36.0	40.0	30.0	41.0
36	36.72841729317667	39.0	36.0	40.0	30.0	41.0
37	36.70478966162262	39.0	35.0	40.0	30.0	41.0
38	36.67381028344154	38.0	35.0	40.0	30.0	41.0
39	36.60289085187744	38.0	35.0	40.0	30.0	41.0
40	36.54769207481974	38.0	35.0	40.0	30.0	41.0
41	36.49572746602884	38.0	35.0	40.0	30.0	41.0
42	36.45319785972048	38.0	35.0	40.0	30.0	41.0
43	35.265600441574556	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	1.0
12	3.0
13	1.0
14	1.0
15	5.0
16	13.0
17	32.0
18	57.0
19	141.0
20	271.0
21	540.0
22	944.0
23	1680.0
24	2716.0
25	4411.0
26	6486.0
27	9458.0
28	13644.0
29	18969.0
30	26136.0
31	34363.0
32	46156.0
33	61457.0
34	84791.0
35	121992.0
36	185777.0
37	294655.0
38	418953.0
39	199035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.594394064841254	17.447218856695095	12.527785987706638	27.430601090757012
2	18.57705730486848	20.040882183497455	35.26776655790414	26.114293953729927
3	20.24118269951595	21.7802996939382	29.315383275363622	28.66313433118222
4	15.890290122574783	14.809841736494558	33.0757481746229	36.224119966307754
5	16.306158360067254	35.19580190996655	33.06576577105586	15.43227395891034
6	37.14080473831422	32.91459433925949	14.565174982400306	15.379425940025982
7	30.650480819105148	29.897820639043466	20.169935740723705	19.281762801127687
8	27.80588726930372	34.990151347591855	18.925658496101967	18.278302887002454
9	28.643300012983648	14.254219849063448	17.430646761884443	39.67183337606846
10	16.910713598317216	26.395106142962373	32.337572266403605	24.35660799231681
11	37.92974084244962	20.84632513797574	19.988947587161714	21.23498643241293
12	22.58345909204494	23.29729881379033	29.183067494749455	24.936174599415278
13	32.12924668085081	18.773443152821464	23.019618359010295	26.07769180731743
14	23.524153828186524	19.000624389556446	24.22044278810489	33.25477899415214
15	27.092960970106944	25.96775487914441	21.128703194434387	25.81058095631426
16	25.66671690490684	25.01127098180527	23.572630467731067	25.749381645556817
17	25.51182653844824	24.601274746411065	23.95137460306897	25.93552411207173
18	25.499886800848437	24.92932393030064	24.54901392773653	25.021775341114388
19	26.88704942986122	24.522198451487803	24.142214671262487	24.448537447388492
20	25.782395346242602	23.87210257474243	24.165767924123294	26.17973415489167
21	25.35602274944102	24.806990553253513	24.89317849516245	24.94380820214302
22	26.406001969078037	23.761317468488553	23.455386159169098	26.37729440326431
23	25.499169111703097	24.089888608120198	24.356868970187843	26.054073309988862
24	25.15278623237339	23.761969913166137	24.552667617931007	26.532576236529465
25	25.969451235306128	23.443120199230506	23.94374100034123	26.643687565122132
26	25.492840398330525	23.83641385087855	24.68035104133433	25.9903947094566
27	26.508566272394347	24.260111424502036	23.84561332083249	25.385708982271122
28	25.753883513832154	25.000701378028406	23.146062137526204	26.09935297061324
29	25.579615540449286	24.85559768173357	24.40436694271601	25.160419835101134
30	26.755255604989113	24.11520346161048	23.516324492055507	25.6132164413449
31	25.85566488353536	23.997632930709724	24.435619042772316	25.7110831429826
32	25.36228621834583	24.26539622639047	24.101045412106895	26.271272143156803
33	25.512348494190306	23.73678554861137	24.143650049553173	26.60721590764515
34	25.957576742174087	24.003113466001434	24.930302597317016	25.10900719450746
35	25.764583606544544	23.713036562347288	24.557038997270826	25.965340833837345
36	25.41539521510122	23.488530348790402	25.103657148151264	25.99241728795711
37	24.661756368023163	23.29410183487017	25.716694167209823	26.327447629896845
38	25.513327161206682	22.730259144501492	25.364961241523925	26.3914524527679
39	24.290515346477484	23.119116172341958	26.546147085823225	26.044221395357336
40	25.56284771223536	22.25762822005915	26.568330204861105	25.611193862844384
41	24.49068537356046	22.75700937628246	26.686161713632895	26.066143536524176
42	23.91855663578646	22.873992706973397	27.176669622241057	26.03078103499909
43	23.562321841825227	22.450947578027492	26.77887410231768	27.207856477829605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	30.5
2	36.0
3	79.0
4	122.0
5	122.0
6	186.0
7	250.0
8	242.0
9	234.0
10	323.0
11	412.0
12	412.0
13	713.0
14	1014.0
15	1520.5
16	2027.0
17	2026.0
18	2025.0
19	2025.0
20	2469.0
21	2913.0
22	3332.5
23	3752.0
24	5005.0
25	6258.0
26	6258.0
27	8001.5
28	9745.0
29	12015.0
30	14285.0
31	18044.0
32	21803.0
33	21803.0
34	26486.0
35	31169.0
36	37158.5
37	43148.0
38	50255.0
39	57362.0
40	57362.0
41	65706.0
42	74050.0
43	83231.5
44	92413.0
45	104761.0
46	117109.0
47	117109.0
48	130075.5
49	143042.0
50	151818.0
51	160594.0
52	162608.5
53	164623.0
54	164623.0
55	154407.0
56	144191.0
57	132262.0
58	120333.0
59	111831.5
60	103330.0
61	103330.0
62	90265.5
63	77201.0
64	64661.5
65	52122.0
66	43322.0
67	34522.0
68	34522.0
69	27984.5
70	21447.0
71	17317.0
72	13187.0
73	10494.5
74	7802.0
75	7802.0
76	6268.5
77	4735.0
78	3755.0
79	2775.0
80	2116.5
81	1458.0
82	1458.0
83	1067.0
84	676.0
85	503.5
86	331.0
87	224.5
88	118.0
89	118.0
90	78.5
91	39.0
92	27.0
93	15.0
94	9.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1532697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.36657979981487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41104118331305	56.86483141861118
2	8.499321459821441	11.451404347346804
3	2.7617111372609653	5.581411011369849
4	1.3579895757839788	3.659324524974726
5	0.7749150676979187	2.6101688873075397
6	0.49121088955207054	1.9854718553728568
7	0.3541413493362531	1.6700104029332368
8	0.25597560187202223	1.3795360648253783
9	0.18740577654740617	1.1362397580654402
>10	0.8592130481974771	9.502382059108932
>50	0.02923175255563514	1.3652604775236947
>100	0.01648563025356242	2.0637802520749267
>500	0.0011635952642592444	0.5698113166804191
>1k	1.9393254404320742E-4	0.1603676238049328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.52444677584676E-5
7	0.0	0.0	0.0	0.0	6.52444677584676E-5
8	0.0	0.0	0.0	0.0	6.52444677584676E-5
9	0.0	0.0	0.0	1.304889355169352E-4	6.52444677584676E-5
10	0.0	0.0	0.0	1.304889355169352E-4	6.52444677584676E-5
11	0.0	0.0	0.0	1.9573340327540278E-4	6.52444677584676E-5
12	0.0	0.0	0.0	1.9573340327540278E-4	6.52444677584676E-5
13	0.0	0.0	0.0	2.609778710338704E-4	6.52444677584676E-5
14	0.0	0.0	0.0	3.9146680655080556E-4	6.52444677584676E-5
15	0.0	0.0	0.0	3.9146680655080556E-4	1.9573340327540278E-4
16	0.0	0.0	0.0	3.9146680655080556E-4	2.609778710338704E-4
17	0.0	0.0	0.0	5.872002098262083E-4	2.609778710338704E-4
18	0.0	0.0	0.0	7.829336131016111E-4	3.2622233879233797E-4
19	0.0	0.0	0.0	9.134225486185463E-4	3.2622233879233797E-4
20	0.0	0.0	0.0	0.0011744004196524167	3.9146680655080556E-4
21	0.0	0.0	0.0	0.0015006227584447546	4.5671127430927316E-4
22	0.0	0.0	0.0	0.0019573340327540276	4.5671127430927316E-4
23	0.0	0.0	0.0	0.0023488008393048334	5.219557420677408E-4
24	0.0	0.0	0.0	0.0031317344524064445	5.219557420677408E-4
25	0.0	0.0	0.0	0.0037189346622326525	5.219557420677408E-4
26	0.0	0.0	0.0	0.0058067576305036155	5.872002098262083E-4
27	0.0	0.0	0.0	0.013766582697036662	5.872002098262083E-4
28	0.0	0.0	0.0	0.04332232659162248	5.872002098262083E-4
29	0.0	0.0	0.0	0.09303861102357479	5.872002098262083E-4
30	0.0	0.0	0.0	0.16200201344427503	5.872002098262083E-4
31	0.0	0.0	0.0	0.5049269359827807	5.872002098262083E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	60	3.7273305E-5	21.583334	5
CTTATAC	900	0.0	17.883333	37
TACTTAC	445	0.0	17.044943	31
GTACTTA	480	0.0	16.1875	30
ATACCGT	80	3.3834856E-4	16.1875	6
TCTTATA	1545	0.0	15.805826	37
GTACTAG	115	2.2106837E-5	14.478261	1
GTCTTAT	145	8.914758E-7	14.034484	1
CTCGTTA	490	0.0	13.969389	22
GTATTAG	120	3.3017703E-5	13.875001	1
CTATACC	120	3.3017703E-5	13.875001	4
AATAGGT	80	0.006299884	13.875001	7
TTTTACG	80	0.006299884	13.875001	2
CTCTTAT	2420	0.0	13.5309925	37
GTGTACT	615	0.0	13.235772	28
CGTTACA	140	9.563008E-6	13.214285	24
TATAGTA	155	1.8884857E-6	13.129032	5
GTACTAC	100	0.0018329648	12.950001	1
TCTATAC	100	0.0018329648	12.950001	3
AGTGTAC	630	0.0	12.920635	27
>>END_MODULE